Description

Longzyme® β-Amylase CAS 9000-91-3

Principle

β-Amylase is an exonuclease that starts from the non-reducing end of liquefied starch and hydrolyzes it sequentially according to maltose units, while Walden transposition reaction occurs, so that the product is transformed from α-type to B-type maltose. It cannot hydrolyze the a-1,6-glycosidic bond at the branch of starch, and the decomposition of starch stops at 2~3 glucose residues before the 1,6 bond, so the maltose produced is basically glucose-free.

Product Characteristics
1、Temperature range:Stable below 65°C,the most suitable temperature is between 55°C-60°C.
2, PH value range: effective pH range 3.0-6.5, optimal PH value range 5.0-5.6.
3、Effects of common metal ions on enzyme activity: lower concentration of CaMg has activating effect, Hg, Fe and oxidizing agents have inhibiting effect on enzyme activity.

Product properties

1, Product specification: brown liquid, enzyme activity 700000u/mL.
Liquid enzyme pH(25℃℃):4.0-6.0, capacity:≤1.25g/mL.
2、Definition of enzyme activity:1mL of enzyme liquid (or 19 enzyme powder) at
Under the condition of pH5.5 and 60℃, the number of maltose mg formed by hydrolyzing 1.10% starch per hour is one enzyme activity unit, which is expressed by u/mL(u/g).
3、Product standard:Implement our standard LDZJ03.04-2010.

Application method

The best saccharification conditions depend on saccharification time, pH, temperature, substrate concentration and enzyme dosage. Each application manufacturer should decide the optimal process conditions according to their own conditions, and the general recommended methods are as follows.
1, in maltose syrup (syrup), beer brewing, 0.1-0.3L/t dry substrate optimal conditions for the former 12-30 hours, the latter saccharification 0.5-1 hours.
2, high maltose syrup (>70%): need to be synergized with Pullulanase saccharification. Suggested usage: B-amylase 0.5-1.0L/t kilobase, Pullulanase 0.5-1.0L/t kilobase.
0.5-1.0L/t dry base (enzyme activity is counted as 1k unit), saccharification for 24 hours.
3、Ultra high maltose syrup (>90%): need to be synergized with Pullulanase and maltotriose enzyme. Suggested usage: β-amylase 1.5-2.0L/t dry base, Pullulanase 1.5-2.8L/t kilobase (enzyme activity in 1,000 units), saccharification for 24 hours, add maltotriose enzyme 1.5-2.0L/t kilobase, and continue saccharification for 24 hours.
Packing and storage
Packed in liquid plastic drum, 28kg/drum. This product is a biologically active substance, strong light, high temperature, strong acid, strong alkali will cause enzyme inactivation, should be prevented from direct sunlight should be placed in a low-temperature dry place.

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