Description

Longzyme® Compound Glucoamylase CAS 9032-08-0

Compound Glucoamylase Product Specification

Compound Glucoamylase Reaction Parameters

What is Compound Glucoamylase?

1. The compound saccharification enzyme is a compound saccharification enzyme composed of glucoamylase and pullulanone from which transsidase is removed. Among them, glucoamylase is refined by submerged fermentation and refining of Aspergillus niger, and pullulanase is refined by submerged fermentation and refining of Bacillus licheniformis. It is widely used in beer, monosodium glutamate, starch sugar, antibiotics and other industries that require maximum conversion of starch into glucose.

2. Compound Glucoamylase, also known as glucoamylase, can hydrolyze the α-1,4 glucosidic bond of starch from the non-reducing end to produce glucose, and it can also slowly hydrolyze the α-1,6 glycosidic bond to convert it into linear polysaccharides. Pullulanase is a thermostable debranching enzyme (starch a-1.6-glucosidase) used at low pH. It can hydrolyze the a-1,6-D-glycosidic bond in liquefied starch to produce a protein containing 1, The linear polysaccharide with 4-α-D-glucosidic bond can act on amylopectin quickly and effectively, and efficiently cooperate with glucoamylase to accelerate the reaction speed and increase the glucose content.

Product characteristics of Compound Glucoamylase

1. Temperature range: The effective temperature range is 40-65℃, the most suitable temperature is 55-60℃.

2. pH range: the effective pH range is 4.0-5.5, and the optimum pH range is 4.2-4.6.

3. The effect of metal ions on enzyme activity: Mg can activate the enzyme; Cu\M²Fe’ has an inhibitory effect; Zn promotes the activity of pullulanase, but inhibits the activity of glucoamylase.

Product properties of Compound Glucoamylase

1. Product specifications: As a composite enzyme, the performance of this enzyme preparation is shown in the mutual enhancement effect of all the main and secondary enzyme activities, and cannot be evaluated based on the marked activity alone. Taking starch as the substrate as the standard, the specification is 10000u/mL.

Liquid enzyme pH (25°C): 3.0-5.0, bulk density: ≤1.20g/mL.

2. Definition of enzyme activity: 1mL enzyme solution or 1g enzyme powder hydrolyzes soluble starch at 40℃ and pH 4.6 to produce 1mg glucose, which is an enzyme activity unit, the symbol is: u/mL (or u/g ).

3. Product standard: implement Chinese national standard GB 8276-2006.

Compound Glucoamylase application

1. When producing glucose, after the raw material is liquefied, adjust the pH to 4.2-4.5, cool to 58-60℃, add compound glucoamylase, generally add 0.4-0.9L/t dry starch, saccharification time is 30-60 hour.

2. In industries with moderate starch conversion such as beer, the recommended dosage is 0.15-0.35L/t dry starch.

Compound Glucoamylase Benefits

• Maximizes conversion of starch substrates to fermentable sugars
• Produces highly attenuated beers in a cost-effective manner
• Maintain consistent fermentability, regardless of varying raw material qualities

Compound Glucoamylase Dosage

The recommended dosage is 0.4-1.0L of the enzyme preparation per ton of total raw materials, added at mashing-in. However, the optimal dosage depends on the specific process parameters and should be determined by testing different dosages at the brewery.

Compound Glucoamylase Recommended Storage

Compound Glucoamylase Packaging

25kgs/drum; 1,125kgs/drum.

Compound Glucoamylase Safety and Handling Precautions

1. Enzyme preparations are proteins, which may induce sensitization and cause allergic type reactions in sensitized individuals. Prolonged contact may cause minor irritation for skin, eyes or nasal mucosa, so any direct contiguity with human body should be avoided. If irritation or allergic response for skin or eyes develops, consult a doctor.

2. This product is a biologically active substance, high temperature, strong acid, strong alkali will cause enzyme inactivation. Therefore, during transportation and storage, liquid enzymes should be protected from sunlight, and solid enzymes should be protected from sunlight and rain. The warehouse should be kept clean, cool and dry.

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