Compound Glucoamylase CAS 9032-08-0

Compound Glucoamylase Quick Details

Product Name: Compound Glucoamylase

CAS No: 9032-08-0

Appearance: Brown Liquid

Enzyme activity: 150,000 u/ml Or customize other levels

Description

Compound Glucoamylase Characteristics

Declared Activity

150,000 u/ml

Production Organism

Aspergillus niger

Physical Form

Liquid

Color

Brown
Color may vary from batch to batch. Color intensity is not an indication of enzyme activity.

Odour

Normal microbial fermentation odour

Production Method

Produced by submerged fermentation, separation and extraction technology.

Definition of Unit

1 unit of Compound Glucoamylase equals to the amount of enzyme which hydrolyzes soluble starch to get 1mg glucose at 40℃ and pH4.6 in 1h.

 

Compound Glucoamylase Product Specification

Lower Limit

Upper Limit

Enzyme activity

150,000 u/ml

Lead

5 mg/kg

Arsenic

3 mg/kg

Total viable count

50,000 CFU/ml

Coliform Bacteria

30 CFU/ml

Escherichia coli

10 CFU/ml
3 MPN/ml

Salmonella

Not Detected/25ml

 

Compound Glucoamylase Reaction Parameters

Activity Temperature

30℃-65℃

Optimum Temperature

55℃-60℃

Activity pH

3.5-6.0

Optimum pH

4.0-5.0

 

What is Compound Glucoamylase?

 

1. The compound saccharification enzyme is a compound saccharification enzyme composed of glucoamylase and pullulanone from which transsidase is removed. Among them, glucoamylase is refined by submerged fermentation and refining of Aspergillus niger, and pullulanase is refined by submerged fermentation and refining of Bacillus licheniformis. It is widely used in beer, monosodium glutamate, starch sugar, antibiotics and other industries that require maximum conversion of starch into glucose.

2. Compound Glucoamylase, also known as glucoamylase, can hydrolyze the α-1,4 glucosidic bond of starch from the non-reducing end to produce glucose, and it can also slowly hydrolyze the α-1,6 glycosidic bond to convert it into linear polysaccharides. Pullulanase is a thermostable debranching enzyme (starch a-1.6-glucosidase) used at low pH. It can hydrolyze the a-1,6-D-glycosidic bond in liquefied starch to produce a protein containing 1, The linear polysaccharide with 4-α-D-glucosidic bond can act on amylopectin quickly and effectively, and efficiently cooperate with glucoamylase to accelerate the reaction speed and increase the glucose content.

 

Product characteristics of Compound Glucoamylase

 

1. Temperature range: The effective temperature range is 40-65℃, the most suitable temperature is 55-60℃.

2. pH range: the effective pH range is 4.0-5.5, and the optimum pH range is 4.2-4.6.

3. The effect of metal ions on enzyme activity: Mg can activate the enzyme; Cu\M²Fe’ has an inhibitory effect; Zn promotes the activity of pullulanase, but inhibits the activity of glucoamylase.

 

Product properties of Compound Glucoamylase

 

1. Product specifications: As a composite enzyme, the performance of this enzyme preparation is shown in the mutual enhancement effect of all the main and secondary enzyme activities, and cannot be evaluated based on the marked activity alone. Taking starch as the substrate as the standard, the specification is 10000u/mL.

Liquid enzyme pH (25°C): 3.0-5.0, bulk density: ≤1.20g/mL.

2. Definition of enzyme activity: 1mL enzyme solution or 1g enzyme powder hydrolyzes soluble starch at 40℃ and pH 4.6 to produce 1mg glucose, which is an enzyme activity unit, the symbol is: u/mL (or u/g ).

3. Product standard: implement Chinese national standard GB 8276-2006.

 

Compound Glucoamylase application

 

1. When producing glucose, after the raw material is liquefied, adjust the pH to 4.2-4.5, cool to 58-60℃, add compound glucoamylase, generally add 0.4-0.9L/t dry starch, saccharification time is 30-60 hour.

2. In industries with moderate starch conversion such as beer, the recommended dosage is 0.15-0.35L/t dry starch.

Compound Glucoamylase Benefits

• Maximizes conversion of starch substrates to fermentable sugars
• Produces highly attenuated beers in a cost-effective manner
• Maintain consistent fermentability, regardless of varying raw material qualities

 

Compound Glucoamylase Dosage

The recommended dosage is 0.4-1.0L of the enzyme preparation per ton of total raw materials, added at mashing-in. However, the optimal dosage depends on the specific process parameters and should be determined by testing different dosages at the brewery.

Compound Glucoamylase Recommended Storage

Best before

When stored as recommended, the product is best used within 12 months from date of delivery.

Storage at customer’s warehouse

0-15℃

Storage Conditions

This product should be stored in a cool and dry place in sealed container, avoiding insolation, high temperature and damp. The product has been formulated for optimal stability. Extended storage or adverse conditions such as higher temperature or higher humidity may lead to a higher dosage requirement.

 

Compound Glucoamylase Packaging

25kgs/drum; 1,125kgs/drum.

 

Compound Glucoamylase Safety and Handling Precautions

1. Enzyme preparations are proteins, which may induce sensitization and cause allergic type reactions in sensitized individuals. Prolonged contact may cause minor irritation for skin, eyes or nasal mucosa, so any direct contiguity with human body should be avoided. If irritation or allergic response for skin or eyes develops, consult a doctor.

2. This product is a biologically active substance, high temperature, strong acid, strong alkali will cause enzyme inactivation. Therefore, during transportation and storage, liquid enzymes should be protected from sunlight, and solid enzymes should be protected from sunlight and rain. The warehouse should be kept clean, cool and dry.

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