1. 🧪 Clinical Diagnosis and Testing
Catalyzes the reaction of cholesterol to produce a detectable signal (such as hydrogen peroxide) for quantitative analysis.

• Lipid testing: Measurement of total cholesterol and free cholesterol in serum, a core reagent in clinical biochemical analysis.
• Reagent kit development: Used as a key component in commercial in vitro diagnostic reagent kits.

2. 🌿 Biopesticides
Damages cholesterol in the cell membranes of insect intestines, leading to insect death.
• Insect-resistant genes: Introducing cholesterol oxidase genes into crops (such as cotton) to cultivate insect-resistant transgenic varieties.
• Biological insecticides: Effective against specific pests such as boll weevils and tobacco budworms, and can complement traditional Bt proteins.

3. 🍗 Food Industry
Degrades cholesterol in food, reducing its content.
Used in the production of low-cholesterol health foods, such as processed meat and dairy products, to optimize dietary structure.

4. 🧬 Basic Research
Used as a specific catalytic or labeling tool.
• Biocatalysis: Used to produce specific steroid derivatives.
• Cell membrane research: Used as a molecular probe to study the structure and cholesterol distribution of cell membranes.