Bromocresol green Application scenarios
1. 🟢 Acid-Base Indicators
• Acid-Base Titration: Used to indicate the titration endpoint, especially in reactions within the pH range of 3.8-5.4.
• Preparation of Mixed Indicators: For example, mixed with methyl red for standardizing hydrochloric acid solutions, with a color change point at pH 5.1-5.2 (dark red → green).
• pH Measurement: Its sodium salt aqueous solution can be used as a colorimetric reagent for pH determination in spectrophotometry.
2. 🧪 Biological and Chemical Analysis
• Clinical Biochemical Testing: Most commonly used to determine serum albumin concentration, an important diagnostic indicator for assessing liver and kidney function.
• Chromatography and Photometric Analysis: Used as a reagent in thin-layer chromatography for the determination of aliphatic hydroxy acids and alkaloids, or as an extraction and separation agent in photometric determination of quaternary ammonium cations.
• Detection of Specific Substances: For example, used in the acid dye colorimetric method to determine the content of atropine sulfate tablets.
3. 🔬 Other Professional and Industrial Uses
• Cell Staining: Used as a biological staining agent. • Environmental monitoring: Participated in the preparation of mixed indicator test strips for determining ammonia concentration in the air.
• Industrial analysis: Used to test the content of free amines in substances such as NMP (N-methylpyrrolidone).





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