1. Gel Electrophoresis Tracking Dye
In agarose gel or polyacrylamide gel electrophoresis, its migration rate is similar to that of specific sizes of nucleic acids or proteins, and it is used to visually monitor the electrophoresis process.

2. Acid-Base Indicator
It exhibits a significant color change in the pH range of 3.0 (yellow) to 4.6 (bluish-purple), and is commonly used for determining the pH of solutions.

3. Protein Blotting
As a key component of the loading buffer, it makes the sample visible and easy to load during electrophoresis.

4. Specific Protein Staining
It can be used for staining specific tissues such as muscle striations, or for protein quantitative analysis studies.

5. Blood-Brain Barrier Staining
It is used as a well-known blood-brain barrier detection stain.

6. Environmental Tracing and Pollutant Studies
It is used as a tracer in wastewater treatment research; at the same time, as a dye pollutant itself, it is a common model pollutant in photocatalytic degradation technology research.