\u9176\u7684\u7ec4\u6210<\/p>\n
\u7b80\u5355\u9176\uff1a\u4ec5\u7531\u6c28\u57fa\u9178\u6b8b\u57fa\u7ec4\u6210\u7684\u9176\u3002<\/p>\n
\u7ed3\u5408\u9176\uff1a\u7531\u9176\u86cb\u767d\u548c\u975e\u86cb\u767d\u8f85\u52a9\u56e0\u5b50\u7ec4\u6210<\/p>\n
\u9176\u86cb\u767d\uff1a\u51b3\u5b9a\u53cd\u5e94\u7684\u7279\u5f02\u6027\uff1b<\/p>\n
\u8f85\u52a9\u56e0\u5b50\uff1a\u51b3\u5b9a\u53cd\u5e94\u7684\u7c7b\u578b\u548c\u6027\u8d28\uff1b\u53ef\u4ee5\u662f\u91d1\u5c5e\u79bb\u5b50\u6216\u5c0f\u578b\u6709\u673a\u5316\u5408\u7269\u3002<\/p>\n
<\/p>\n
\u8f85\u52a9\u56e0\u5b50<\/p>\n
\u8f85\u52a9\u56e0\u5b50\uff1a\u4e0e\u9176\u86cb\u767d\u677e\u6563\u7ed3\u5408\uff0c\u53ef\u901a\u8fc7\u900f\u6790\u6216\u8d85\u6ee4\u53bb\u9664\u3002<\/p>\n
\u8f85\u52a9\u56e0\u5b50\uff1a\u4e0e\u9176\u86cb\u767d\u7d27\u5bc6\u7ed3\u5408\uff0c\u4e0d\u80fd\u901a\u8fc7\u900f\u6790\u6216\u8d85\u6ee4\u53bb\u9664\u3002<\/p>\n
\u9176\u86cb\u767d\u4e0e\u8f85\u56e0\u5b50\u7ed3\u5408\u5f62\u6210\u7684\u590d\u5408\u7269\u79f0\u4e3a\u5168\u9176\uff0c\u53ea\u6709\u5168\u9176\u624d\u5177\u6709\u50ac\u5316\u4f5c\u7528\u3002<\/p>\n
<\/p>\n
\u9176\u7684\u6d3b\u6027\u4e2d\u5fc3<\/p>\n
\u9176\u5fc5\u9700\u57fa\u56e2\uff1a\u9176\u53d1\u6325\u6d3b\u6027\u6240\u5fc5\u9700\u7684\u57fa\u56e2<\/p>\n
\u9176\u7684\u6d3b\u6027\u4e2d\u5fc3\uff1a\u5728\u4e00\u7ea7\u7ed3\u6784\u4e2d\u76f8\u8ddd\u5f88\u8fdc\uff0c\u4f46\u5728\u7a7a\u95f4\u7ed3\u6784\u4e2d\u4e00\u4e9bR\u57fa\u56e2\u76f8\u4e92\u9760\u8fd1\u5f62\u6210\u4e00\u4e2a\u7279\u6b8a\u533a\u57df\uff0c\u8be5\u533a\u57df\u80fd\u7279\u5f02\u6027\u5730\u7ed3\u5408\u5e95\u7269\u5e76\u50ac\u5316\u5e95\u7269\u53d1\u751f\u5316\u5b66\u53d8\u5316\u3002<\/p>\n
\u6d3b\u52a8\u4e2d\u5fc3\u5fc5\u987b\u5206\u4e3a\u4ee5\u4e0b\u7ec4\u522b<\/p>\n
\u7ed3\u5408\u57fa\u56e2\uff1a\u53c2\u4e0e\u9176\u4e0e\u5e95\u7269\u7684\u7ed3\u5408<\/p>\n
\u50ac\u5316\u7ec4\uff1a\u50ac\u5316\u5e95\u7269\u8f6c\u5316\u4e3a\u4ea7\u7269\u3002<\/p>\n
\u6d3b\u52a8\u4e2d\u5fc3\u5916\u7684\u5fc5\u9009\u7ec4\uff1a\u6d3b\u52a8\u4e2d\u5fc3\u5185\u5fc5\u987b\u6709\u4e00\u4e2a\u5fc5\u9009\u7ec4\uff0c\u4f46\u5fc5\u9009\u7ec4\u4e0d\u4e00\u5b9a\u603b\u662f\u5728\u6d3b\u52a8\u4e2d\u5fc3\u5185\u3002\u6d3b\u52a8\u4e2d\u5fc3\u5916\u7684\u5fc5\u9009\u7ec4\u8d77\u5230\u7a33\u5b9a\u6d3b\u52a8\u4e2d\u5fc3\u7684\u4f5c\u7528\u3002<\/p>\n
<\/p>\n
\u9176\u4e0e\u666e\u901a\u50ac\u5316\u5242\u7684\u533a\u522b<\/p>\n
\u9ad8\u6548\u7387\uff1a\u9176\u7684\u50ac\u5316\u4f5c\u7528\u53ef\u4f7f\u53cd\u5e94\u901f\u7387\u63d0\u9ad8 10^6 \u5230 10^12 \u500d\uff0c\u53cd\u5e94\u524d\u540e\u9176\u672c\u8eab\u6ca1\u6709\u53d8\u5316\uff0c\u9ad8\u6548\u7387\u5c31\u662f\u964d\u4f4e\u53cd\u5e94\u7684\u6d3b\u5316\u80fd<\/p>\n
\u7279\u5f02\u6027\uff08\u5bf9\u5e95\u7269\u7684\u9009\u62e9\u6027\uff09<\/p>\n
\u7edd\u5bf9\u7279\u5f02\u6027\uff1a\u9176\u5bf9\u5e95\u7269\u7684\u8981\u6c42\u975e\u5e38\u4e25\u683c\uff0c\u53ea\u5bf9\u7279\u5b9a\u5e95\u7269\u8d77\u4f5c\u7528\uff1b<\/p>\n
\u76f8\u5bf9\u7279\u5f02\u6027\uff1a\u4f5c\u7528\u5bf9\u8c61\u4e0d\u662f\u5e95\u7269\uff0c\u800c\u662f\u4e00\u7c7b\u5316\u5408\u7269\u6216\u5316\u5b66\u952e\uff1b<\/p>\n
\u2162 \u7acb\u4f53\u5f02\u6784\u4f53\u7279\u5f02\u6027\uff1aD-\u3001L-\u3001\u987a\u53cd\u5f0f<\/p>\n
\u9176\u6d3b\u6027\u4e0d\u7a33\u5b9a\uff1a\u86cb\u767d\u8d28\u5bb9\u6613\u53d8\u6027\u548c\u5931\u6d3b<\/p>\n
\u9176\u6d3b\u6027\u53ef\u8c03\u8282\u548c\u63a7\u5236\uff1a\u2160\u5f02\u4f4d\u8c03\u8282\uff1b\u2161\u53cd\u9988\u8c03\u8282\uff1b\u2162\u4ef7\u4f9d\u8d56\u4fee\u9970\u8c03\u8282\uff1b\u2163\u9176\u539f\u6fc0\u6d3b\u548c\u6fc0\u7d20\u63a7\u5236<\/p>\n
<\/p>\n
\u8bf1\u5bfc\u9002\u5e94\u7406\u8bba<\/p>\n
\u9176\u8868\u9762\u6ca1\u6709\u4e0e\u5e95\u7269\u4e92\u8865\u7684\u56fa\u5b9a\u5f62\u72b6\uff0c\u53ea\u662f\u5728\u5e95\u7269\u7684\u8bf1\u5bfc\u4e0b\u5f62\u6210\u4e92\u8865\u5f62\u72b6\u3002<\/p>\n
<\/p>\n
\u5f71\u54cd\u9176\u4fc3\u53cd\u5e94\u7684\u56e0\u7d20<\/p>\n
(1) \u5e95\u7269\u6d53\u5ea6\uff1b(2) \u6291\u5236\u5242\uff1b(3) \u9176\u6d53\u5ea6\uff1b(4) \u6e29\u5ea6\uff1b(5) pH \u503c\uff1b(6) \u6fc0\u6d3b\u5242\u3002<\/p>\n
<\/p>\n
\u5e95\u7269\u6d53\u5ea6\u5bf9\u9176\u53cd\u5e94\u901f\u7387\u7684\u5f71\u54cd\uff1a<\/p>\n
\u4e2d\u95f4\u4ea7\u7269\u5b66\u8bf4\uff1a\u9176\u50ac\u5316\u65f6\uff0c\u9176\u7684\u6d3b\u6027\u4e2d\u5fc3\u9996\u5148\u4e0e\u9176\u5e95\u7269\u7ed3\u5408\uff0c\u5f62\u6210\u9176\u548c\u5e95\u7269\u7684\u590d\u5408\u7269\uff0c\u7136\u540e\u5206\u89e3\u91ca\u653e\u9176\uff0c\u91ca\u653e\u4ea7\u7269<\/p>\n
\u7c73\u6c0f\u65b9\u7a0bV=Vmax\u00d7[S]\/(Km+[S])<\/p>\n
(1) \u5f53\u5e95\u7269\u6d53\u5ea6\u975e\u5e38\u5927\u65f6\uff08[S]\u226510\u00d7Km\uff09\uff0c\u9176\u4e0e\u5e95\u7269\u7684\u53cd\u5e94\u8fbe\u5230\u9971\u548c\uff0c\u53cd\u5e94\u901f\u7387\u8fbe\u5230\u6700\u5927\u503c\u3002<\/p>\n
(2) \u5f53\u53cd\u5e94\u901f\u5ea6 V=1\/2Vmax \u65f6\uff0cKm=[S]\u3002<\/p>\n
\u7c73\u6c0f\u65b9\u7a0b\u4e2d\u52a8\u529b\u5b66\u53c2\u6570 Km \u7684\u610f\u4e49\u2605......<\/p>\n
\u5728\u6570\u503c\u4e0a\uff0c\u2460Km \u7b49\u4e8e\u6700\u5927\u53cd\u5e94\u901f\u7387\u4e00\u534a\u6240\u5bf9\u5e94\u7684\u5e95\u7269\u6d53\u5ea6\uff0c\u5373\u5f53 V=1\/2Vmax \u65f6\uff0cKm=[S]\u3002<\/p>\n
\u2461Km \u5355\u4f4d\uff1a\u6469\u5c14\/\u5347<\/p>\n
\u4e0d\u540c\u7684\u9176\u6709\u4e0d\u540c\u7684 Km \u503c\uff0c\u8fd9\u662f\u9176\u7684\u4e00\u4e2a\u91cd\u8981\u7279\u5f81\u7269\u7406\u5e38\u6570\u3002<\/p>\n
\u540c\u4e00\u79cd\u9176\u5bf9\u4e0d\u540c\u5e95\u7269\u7684 Km \u503c\u4e0d\u540c\uff0cKm \u503c\u6700\u5c0f\u7684\u5e95\u7269\u88ab\u79f0\u4e3a\u6700\u9002\u5408\u7684\u5e95\u7269\u3002<\/p>\n
Km \u503c\u8d8a\u5927\uff0c\u4eb2\u548c\u529b\u8d8a\u5c0f\uff0c\u50ac\u5316\u6d3b\u6027\u8d8a\u4f4e\uff1bKm \u503c\u8d8a\u5c0f\uff0c\u4eb2\u548c\u529b\u8d8a\u5927\uff0c\u50ac\u5316\u6d3b\u6027\u8d8a\u9ad8\u3002<\/p>\n
<\/p>\n
\u6291\u5236\u5242\u5bf9\u9176\u53cd\u5e94\u901f\u7387\u7684\u5f71\u54cd<\/p>\n
(1) \u4e0d\u53ef\u9006\u7684\u6291\u5236\u4f5c\u7528<\/p>\n
\u6291\u5236\u5242\u4e0e\u9176\u6d3b\u6027\u4e2d\u5fc3\u7684\u6d3b\u6027\u57fa\u56e2\u6216\u5176\u4f4d\u70b9\u4e2d\u7684\u67d0\u4e9b\u57fa\u56e2\u4ee5\u5171\u4ef7\u5f62\u5f0f\u7ed3\u5408\uff0c\u9020\u6210\u9176\u5931\u6d3b\uff0c\u7269\u7406\u65b9\u6cd5\u65e0\u6cd5\u6d88\u9664<\/p>\n
<\/p>\n
(2) \u53ef\u9006\u6291\u5236<\/p>\n
\u2160 \u7ade\u4e89\u6027\u6291\u5236<\/p>\n
a.\u6291\u5236\u5242\u7684\u5316\u5b66\u7ed3\u6784\u4e0e\u5e95\u7269\u76f8\u4f3c\uff0c\u53ef\u4e0e\u5e95\u7269\u7ade\u4e89\u6027\u5730\u7ed3\u5408\u5230\u9176\u7684\u6d3b\u6027\u4e2d\u5fc3\uff1b<\/p>\n
b.\u5f53\u6291\u5236\u5242\u4e0e\u6d3b\u6027\u4e2d\u5fc3\u7ed3\u5408\u65f6\uff0c\u5e95\u7269\u88ab\u6392\u9664\u5728\u53cd\u5e94\u4e2d\u5fc3\u4e4b\u5916\uff0c\u7ed3\u679c\u9176\u4fc3\u53cd\u5e94\u53d7\u5230\u6291\u5236\uff1b<\/p>\n
c.\u63d0\u9ad8\u5e95\u7269\u7684\u6d53\u5ea6\u4f1a\u589e\u5f3a\u5e95\u7269\u7684\u7ade\u4e89\u80fd\u529b\uff08\u5373\u53ef\u4ee5\u91ca\u653e\u6291\u5236\u4f5c\u7528\uff09\uff1b<\/p>\n
D. Km \u503c\u5347\u9ad8\uff0cVmax \u4fdd\u6301\u4e0d\u53d8<\/p>\n
<\/p>\n
II \u975e\u7ade\u4e89\u6027\u6291\u5236<\/p>\n
\u4e0e\u6d3b\u52a8\u4e2d\u5fc3\u4ee5\u5916\u7684\u5f3a\u5236\u7ec4\u7ed1\u5b9a<\/p>\n
Km \u503c\u4fdd\u6301\u4e0d\u53d8\uff0cVmax \u4e0b\u964d<\/p>\n
<\/p>\n
\u53cd\u7ade\u4e89\u6291\u5236<\/p>\n
\u4e0e\u9176-\u5e95\u7269\u590d\u5408\u7269\u7ed3\u5408<\/p>\n
Km \u503c\u4e0b\u964d\uff0cVmax \u4e0b\u964d<\/p>\n
<\/p>\n
\u6fc0\u6d3b\u9176\u539f<\/p>\n
\u9176\u539f\uff1a\u975e\u6d3b\u6027\u9176\u524d\u4f53<\/p>\n
\u6fc0\u6d3b\uff1a\u4e00\u7ea7\u7ed3\u6784\u53d8\u5316\uff0c\u5f15\u8d77\u6784\u8c61\u53d8\u5316\uff0c\u5f62\u6210\u6216\u66b4\u9732\u6d3b\u6027\u4e2d\u5fc3<\/p>\n
<\/p>\n
\u9176\u6d3b\u6027\u8c03\u8282<\/p>\n
\u9176\u7684\u5171\u4ef7\u4fee\u9970\uff08\u5316\u5b66\u4fee\u9970\u8c03\u8282\uff09\uff1a\u4e00\u79cd\u9176\u88ab\u53e6\u4e00\u79cd\u9176\u4fee\u9970\uff0c\u5171\u4ef7\u8fde\u63a5\u4e00\u4e2a\u5316\u5b66\u57fa\u56e2\uff0c\u6216\u5171\u4ef7\u952e\u65ad\u88c2\uff0c\u53bb\u9664\u4e00\u4e2a\u5316\u5b66\u57fa\u56e2\uff0c\u4ece\u800c\u8c03\u8282\u9176\u7684\u6d3b\u6027<\/p>\n
\u5f02\u6784\u8c03\u8282\uff1a\u67d0\u4e9b\u7269\u8d28\u53ef\u9006\u5730\u4e0e\u76f8\u5e94\u9176\u5206\u5b50\u7684\u6d3b\u6027\u4e2d\u5fc3\u6216\u6d3b\u6027\u4e2d\u5fc3\u4ee5\u5916\u7684\u7279\u5b9a\u90e8\u5206\u7ed3\u5408\uff0c\u4f7f\u9176\u7684\u6d3b\u6027\u4e2d\u5fc3\u6784\u8c61\u53d1\u751f\u53d8\u5316\uff0c\u4ece\u800c\u5bfc\u81f4\u529f\u80fd\u53d8\u5316<\/p>\n
<\/p>\n
\u540c\u5de5\u9176<\/p>\n
\u6307\u50ac\u5316\u5316\u5b66\u53cd\u5e94\u76f8\u540c\uff0c\u9176\u86cb\u767d\u7684\u5206\u5b50\u7ed3\u6784\u3001\u7406\u5316\u6027\u8d28\u548c\u514d\u75ab\u5b66\u6027\u8d28\u4e0d\u540c\u7684\u4e00\u7ec4\u9176 \u2461\u8fd9\u7c7b\u9176\u5b58\u5728\u4e8e\u540c\u79cd\u751f\u7269\u6216\u540c\u4e00\u673a\u4f53\u7684\u4e0d\u540c\u7ec4\u7ec7\u751a\u81f3\u540c\u4e00\u7ec4\u7ec7\u6216\u7ec6\u80de\u4e2d<\/p>\n
<\/p>\n
\u4ee5\u80f0\u86cb\u767d\u9176\u4e3a\u4f8b\u8bf4\u660e\u86cb\u767d\u8d28\u7ed3\u6784\u4e0e\u529f\u80fd\u4e4b\u95f4\u7684\u5173\u7cfb<\/p>\n
\u7531\u4e8e\u80f0\u86cb\u767d\u9176\u7684\u4e00\u7ea7\u7ed3\u6784\u76f8\u8ddd\u8f83\u8fdc\uff0c\u80a0\u6fc0\u9176\u5207\u65adN\u7aef6\u80bd\uff0c\u4f7f\u5176\u4e00\u7ea7\u6784\u8c61\u53d1\u751f\u53d8\u5316\uff0c\u5f62\u6210\u4e00\u4e2a\u7279\u6b8a\u533a\u57df\uff0c\u5373\u9176\u7684\u6d3b\u6027\u4e2d\u5fc3\uff0c\u8be5\u533a\u57df\u80fd\u7279\u5f02\u6027\u5730\u7ed3\u5408\u5e95\u7269\uff0c\u5e76\u50ac\u5316\u5e95\u7269\u53d1\u751f\u5316\u5b66\u53d8\u5316\uff0c\u8d77\u5230\u7ed3\u5408\u548c\u50ac\u5316\u7684\u4f5c\u7528\uff0c\u8bf4\u660e\u4e00\u7ea7\u7ed3\u6784\u7684\u6539\u53d8\uff0c\u5f15\u8d77\u6784\u8c61\u7684\u6539\u53d8\uff0c\u5f62\u6210\u6d3b\u6027\u4e2d\u5fc3\uff0c\u4f7f\u80f0\u86cb\u767d\u7531\u65e0\u6d3b\u6027\u53d8\u4e3a\u6709\u6d3b\u6027\u3002<\/p>\n
<\/p>\n
In enzyme and food-processing projects, the most useful decision frame is usually application fit plus process stability: which ingredient performs under the intended pH, temperature, time, and substrate conditions without creating a downstream quality or compliance problem.<\/p>\n
Why is a high-activity enzyme not automatically the best commercial choice?<\/strong> Should food and biotech ingredients be selected from data sheets alone?<\/strong> <\/p>\n <\/p>\n Quick answer:<\/strong> Enzyme and food-processing ingredients are usually selected by substrate fit, pH and temperature window, dosage, and whether the end-use specification is acceptable for the target process. The strongest commercial choice is the one that performs consistently under real processing conditions.<\/p>\n <\/p>\n Composition of enzymes Simple enzymes: enzymes composed only of amino acid residues. Bound enzymes: composed of enzyme proteins and non-protein cofactors Enzyme protein: determines the specificity of the reaction; Cofactors:<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[108],"tags":[],"class_list":["post-7323","post","type-post","status-publish","format-standard","hentry","category-enzyme-news"],"yoast_head":"\n
Because the best enzyme is the one that performs reliably under the actual process conditions and gives the desired downstream result without creating new issues.<\/p>\n
It is usually safer to pair the specification review with a pilot or application test because real substrates and process windows can change the result a lot.<\/p>\n\u7acb\u5373\u8054\u7cfb\u6211\u4eec\uff01<\/b><\/strong><\/h2>\n
\u5982\u679c\u60a8\u9700\u8981\u4ef7\u683c\uff0c\u8bf7\u5728\u4e0b\u8868\u4e2d\u586b\u5199\u60a8\u7684\u8054\u7cfb\u4fe1\u606f\uff0c\u6211\u4eec\u901a\u5e38\u4f1a\u5728 24 \u5c0f\u65f6\u5185\u4e0e\u60a8\u8054\u7cfb\u3002\u60a8\u4e5f\u53ef\u4ee5\u7ed9\u6211\u53d1\u7535\u5b50\u90ae\u4ef6\u00a0info@longchangchemical.com<\/a><\/span>\u00a0\u8bf7\u5728\u5de5\u4f5c\u65f6\u95f4\uff08UTC+8 \u5468\u4e00\u81f3\u5468\u516d\uff0c\u4e0a\u5348 8:30 \u81f3\u4e0b\u5348 6:00\uff09\u6216\u4f7f\u7528\u7f51\u7ad9\u5373\u65f6\u804a\u5929\u5de5\u5177\u83b7\u5f97\u53ca\u65f6\u56de\u590d\u3002<\/b><\/strong><\/h4>\n
\n\n
\n \u590d\u5408\u8461\u8404\u7cd6\u6dc0\u7c89\u9176<\/span><\/a><\/td>\n 9032-08-0<\/td>\n<\/tr>\n \n \u666e\u9c81\u5170\u9176<\/span><\/a><\/td>\n 9075-68-7<\/td>\n<\/tr>\n \n \u6728\u805a\u7cd6\u9176<\/span><\/a><\/td>\n 37278-89-0<\/td>\n<\/tr>\n \n \u7ea4\u7ef4\u7d20\u9176<\/span><\/a><\/td>\n 9012-54-8<\/td>\n<\/tr>\n \n \u67da\u76ae\u82f7\u9176<\/span><\/a><\/td>\n 9068-31-9<\/td>\n<\/tr>\n \n \u03b2-\u6dc0\u7c89\u9176<\/span><\/a><\/td>\n 9000-91-3<\/td>\n<\/tr>\n \n \u8461\u8404\u7cd6\u6c27\u5316\u9176<\/span><\/a><\/td>\n 9001-37-0<\/td>\n<\/tr>\n \n \u03b1-\u6dc0\u7c89\u9176<\/td>\n 9000-90-2<\/td>\n<\/tr>\n \n \u679c\u80f6\u9176<\/span><\/a><\/td>\n 9032-75-1<\/td>\n<\/tr>\n \n \u8fc7\u6c27\u5316\u7269\u9176<\/td>\n 9003-99-0<\/td>\n<\/tr>\n \n \u8102\u80aa\u9176<\/span><\/a><\/td>\n 9001-62-1<\/td>\n<\/tr>\n \n \u8fc7\u6c27\u5316\u6c22\u9176<\/span><\/a><\/td>\n 9001-05-2<\/td>\n<\/tr>\n \n TANNASE<\/span><\/a><\/td>\n 9025-71-2<\/td>\n<\/tr>\n \n \u5f39\u6027\u86cb\u767d\u9176<\/span><\/a><\/td>\n 39445-21-1<\/td>\n<\/tr>\n \n \u5c3f\u7d20\u9176<\/span><\/a><\/td>\n 9002-13-5<\/td>\n<\/tr>\n \n DEXTRANASE<\/span><\/a><\/td>\n 9025-70-1<\/td>\n<\/tr>\n \n L \u4e73\u9178\u8131\u6c22\u9176<\/span><\/a><\/td>\n 9001-60-9<\/td>\n<\/tr>\n \n \u82f9\u679c\u9178\u8131\u6c22\u9176<\/span><\/a><\/td>\n 9001-64-3<\/td>\n<\/tr>\n \n \u80c6\u56fa\u9187\u6c27\u5316\u9176<\/span><\/a><\/td>\n 9028-76-6<\/td>\n<\/tr>\n<\/tbody>\n<\/table>","protected":false},"excerpt":{"rendered":"