{"id":4557,"date":"2021-04-29T10:10:14","date_gmt":"2021-04-29T10:10:14","guid":{"rendered":"https:\/\/longchangchemical.com\/?p=4557"},"modified":"2024-09-28T11:56:32","modified_gmt":"2024-09-28T11:56:32","slug":"immobilized-on-magnetic-silicon-based-materials","status":"publish","type":"post","link":"https:\/\/longchangchemical.com\/tr\/immobilized-on-magnetic-silicon-based-materials\/","title":{"rendered":"Manyetik silikon bazl\u0131 malzemeler \u00fczerine immobilize edilmi\u015f naringinaz\u0131n deneysel parametreleri"},"content":{"rendered":"

1. Naringinaz enzim aktivitesinin tespit y\u00f6ntemi<\/strong><\/h1>\r\n\r\n\r\n\r\n
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1-1 Spektrofotometre y\u00f6ntemi<\/h3>\r\n\r\n\r\n\r\n
\"\"<\/figure>\r\n\r\n\r\n\r\n

Para nitrofenol spektrofotometrik y\u00f6ntem, \u03b1-rhamnosidaz ve \u03b2-glukosidaz\u0131n enzim aktivitesini tespit etmek i\u00e7in substrat olarak p-nitrofenol- \u03b1-rhamnoside (pNP- \u03b1- Rha) ve p-nitrofenol-\u03b2-D-glukopiranoside (pNP- \u03b2-Glu) kullan\u0131r. Li Ping, enzimatik hidroliz i\u00e7in p-nitrofenol -\u03b2-glukozit (pNPG) \u00e7\u00f6zeltisine Aspergillus niger \u03b2-glukozidaz ekleme deneyinde p-nitrofenol spektrofotometre y\u00f6ntemini kulland\u0131 ve absorbans de\u011feri 400 nm ultraviyole dalga boyunda \u00f6l\u00e7\u00fcld\u00fc. Ve absorbans de\u011ferine dayanarak, \u03b2-glukozidaz\u0131n enzim aktivitesini hesaplarlar. Bu y\u00f6ntem g\u00fc\u00e7l\u00fc substrat \u00f6zg\u00fcll\u00fc\u011f\u00fcne sahiptir, ancak standart substrat pahal\u0131d\u0131r ve g\u0131dalardaki glikozitleri hidrolize etmek i\u00e7in naringinaz\u0131n enzim aktivitesi belirlenemez, bu nedenle bu y\u00f6ntem naringinaz\u0131n end\u00fcstriyel b\u00fcy\u00fck \u00f6l\u00e7ekli haz\u0131rlanmas\u0131nda enzim aktivitesinin tespiti i\u00e7in uygun de\u011fildir.<\/h4>\r\n\r\n\r\n\r\n
\"\"<\/figure>\r\n\r\n\r\n\r\n

Davis spektrofotometre y\u00f6nteminin prensibi, naringinin hafif alkali ko\u015fullar alt\u0131nda 90% dietilen glikol ile kromojenik bir reaksiyon reaksiyonu meydana getirebilmesi ve sar\u0131 naringin kalkon \u00fcretiminin 420 nm dalga boyuna sahip ultraviyole \u0131\u015f\u0131k alt\u0131nda tespit edilebilmesidir. \u00d6l\u00e7\u00fclen absorbans de\u011ferine g\u00f6re, kalan substrat naringin miktar\u0131 hesaplanabilir ve ard\u0131ndan naringinaz aktivitesi hesaplama yoluyla elde edilebilir. Wang Weina ve arkada\u015flar\u0131 geli\u015ftirilmi\u015f bir Davis y\u00f6ntemi kullanm\u0131\u015f, substrat olarak naringini alm\u0131\u015f ve enzimatik hidroliz i\u00e7in Aspergillus niger FFCC 848'in s\u0131v\u0131 fermantasyonu ile \u00fcretilen naringinaz\u0131 eklemi\u015ftir. Absorbans 420 nm ultraviyole \u0131\u015f\u0131k alt\u0131nda \u00f6l\u00e7\u00fclm\u00fc\u015f ve ard\u0131ndan enzim aktivitesi hesaplanm\u0131\u015ft\u0131r. Enzim aktivitesini \u00f6l\u00e7mek i\u00e7in Davis spektrofotometre y\u00f6nteminin kullan\u0131m\u0131 basittir, h\u0131zl\u0131d\u0131r, bu nedenle bu y\u00f6ntem, fermantasyon yoluyla naringinaz \u00fcretiminin ve immobilize naringinaz aktivitesinin eeal zaman tespitinde yayg\u0131n olarak kullan\u0131lmaktad\u0131r.<\/h4>\r\n\r\n\r\n\r\n
\"\"<\/figure>\r\n\r\n\r\n\r\n

1-2 Y\u00fcksek performansl\u0131 s\u0131v\u0131 kromatografisi<\/h3>\r\n\r\n\r\n\r\n
\"\"<\/figure>\r\n\r\n\r\n\r\n

Y\u00fcksek performansl\u0131 s\u0131v\u0131 kromatografisinin (HPLC) prensibi, HPLC'nin t\u00fcm hidroliz i\u015flemi s\u0131ras\u0131nda substrat naringin, ara \u00fcr\u00fcn purunin ve nihai \u00fcr\u00fcn naringenin'i do\u011fru bir \u015fekilde belirleyip kantitatif olarak analiz edebilmesi ve ard\u0131ndan enzim aktivitesini hesaplayabilmesidir. Chen Yuelong ve di\u011ferleri, naringin, arbutin, mirisetin ve \u00fcr\u00fcnlerinin al\u0131konma s\u00fcresini belirlemek i\u00e7in HPLC'yi kulland\u0131lar ve 1.5'ten daha y\u00fcksek bir \u00e7\u00f6z\u00fcn\u00fcrl\u00fckle tam ay\u0131rma elde ettiler ve hidroliz s\u00fcrecinde naringinaz\u0131n \u00e7oklu glikozitler \u00fczerindeki etkisini etkili bir \u015fekilde izlediler, ayn\u0131 zamanda enzim aktivitesi do\u011fru bir \u015fekilde belirlendi. HPLC y\u00f6ntemi y\u00fcksek do\u011fruluk avantajlar\u0131na sahiptir ve safs\u0131zl\u0131klar\u0131n giri\u015fimini \u00f6nleyebilir, ancak Davis y\u00f6ntemiyle kar\u015f\u0131la\u015ft\u0131r\u0131ld\u0131\u011f\u0131nda, bu y\u00f6ntem daha uzun bir tespit s\u00fcresine ve daha karma\u015f\u0131k bir i\u015fleme sahiptir.<\/h4>\r\n\r\n\r\n\r\n
\"\"<\/figure>\r\n\r\n\r\n\r\n

1-3 Naringinaz enzim aktivitesi i\u00e7in geli\u015ftirilmi\u015f tayin y\u00f6ntemi<\/h3>\r\n\r\n\r\n\r\n
\"\"<\/figure>\r\n\r\n\r\n\r\n

Geli\u015ftirilmi\u015f Davis y\u00f6ntemini kullanarak: 0,8 mL 0,8 g-L-1<\/sup>\u00a0pH 4,5 tamponu ile haz\u0131rlanan naringin \u00e7\u00f6zeltisi, 40 mg immobilize naringinaz (veya 0,2 mL serbest naringinaz) i\u00e7eren reaksiyon sistemine eklenmi\u015ftir. 30 dakika boyunca 50 \u2103 sabit s\u0131cakl\u0131ktaki su banyosuna yerle\u015ftirin. Reaksiyon enzimatik hidroliz \u00e7\u00f6zeltisinden 0,1 mL al\u0131n ve 5 mL dietilen glikol (90% hacim fraksiyonu) ve 0,1 mL NaOH \u00e7\u00f6zeltisi (4 mol-L-1<\/sup>) t\u00fcplere koyun ve e\u015fit \u015fekilde kar\u0131\u015ft\u0131rd\u0131ktan sonra 10 dakika bekletin. Kar\u0131\u015f\u0131k \u00e7\u00f6zeltinin absorbans\u0131n\u0131 420 nm'de \u00f6l\u00e7\u00fcn.<\/h4>\r\n\r\n\r\n\r\n
\"\"<\/figure>\r\n\r\n\r\n\r\n

Naringinaz enziminin aktivitesinin tan\u0131m\u0131 (U): pH 4.5, 50 \u2103 ko\u015fulunda, 1 enzim aktivitesi birimi, dakikada 1 \u03bcg naringini par\u00e7alamak i\u00e7in gereken enzim miktar\u0131d\u0131r. Enzim aktivite oran\u0131 ( U-g-1<\/sup>\u00a0veya U-mL-1<\/sup>) \u015fu \u015fekilde tan\u0131mlan\u0131r: pH 4.5, 50 \u2103 ko\u015fulunda, 1 g immobilize naringinaz (veya 1 mL serbest naringinaz) taraf\u0131ndan sergilenen enzim aktivitesi. Ba\u011f\u0131l enzim aktivitesi a\u015fa\u011f\u0131daki form\u00fcle g\u00f6re hesaplan\u0131r:<\/h4>\r\n\r\n\r\n\r\n
\"\"<\/figure>\r\n\r\n\r\n\r\n

Ba\u011f\u0131l enzim aktivitesi \/% = enzim aktivitesi\/maksimum enzim aktivitesi\u00d7100<\/h4>\r\n\r\n\r\n\r\n
\"\"<\/figure>\r\n\r\n\r\n\r\n

Standart e\u011frinin belirlenmesi: Standart glikozit naringin \u00e7\u00f6zeltisinin k\u00fctle konsantrasyonu 0, 0.4, 0.8, 1.2, 1.6 g - L olarak ayarlanm\u0131\u015ft\u0131r.-1<\/sup>daha sonra 0,1 mL \u00e7\u00f6zelti al\u0131narak 5 mL dietilen glikol (90% , v\/v) ve 0,1 mL sodyum hidroksit \u00e7\u00f6zeltisi ( 4 mol-L-1<\/sup>). Kar\u0131\u015f\u0131m e\u015fit \u015fekilde kar\u0131\u015ft\u0131r\u0131ld\u0131ktan ve 10 dakika bekletildikten sonra, kar\u0131\u015f\u0131k \u00e7\u00f6zeltinin \u0131\u015f\u0131k absorpsiyon de\u011feri 420 nm'de \u00f6l\u00e7\u00fclm\u00fc\u015f ve buna g\u00f6re standart e\u011fri \u00e7izilmi\u015ftir.<\/h4>\r\n\r\n\r\n\r\n
\"\"<\/figure>\r\n\r\n\r\n\r\n

2. Naringinaz enzim \u00e7\u00f6zeltisinin haz\u0131rlanmas\u0131<\/strong><\/h2>\r\n\r\n\r\n\r\n
\"\"<\/figure>\r\n\r\n\r\n\r\n

4\u00b0C'de saklanan Aspergillus niger FFCC uv-11 e\u011fimli besiyerine aktar\u0131lm\u0131\u015f ve olgun sporlar 96 saat boyunca 30\u00b0C'de sabit s\u0131cakl\u0131k k\u00fclt\u00fcr\u00fc ile elde edilmi\u015ftir. Daha sonra, sporlar 0.9% k\u00fctle fraksiyonu ile steril normal salin ile y\u0131kanm\u0131\u015ft\u0131r. spor s\u00fcspansiyonunun absorbans de\u011feri 600 nm'de \u00f6l\u00e7\u00fclm\u00fc\u015ft\u00fcr (OD600 de\u011feri), steril salin ile OD600 de\u011feri 0.200'e ayarlanm\u0131\u015ft\u0131r. Daha sonra 10% hacim fraksiyonuna sahip spor s\u00fcspansiyonu 100 mL fermantasyon ortam\u0131na (250 mL konik \u015fi\u015fe) inok\u00fcle edilmi\u015ftir, Elde edilen fermantasyon suyu 4 \u2103 ve 6000 r-dk'da santrif\u00fcj edilmi\u015ftir.-1<\/sup>\u00a0so\u011futmal\u0131 santrif\u00fcjde 10 dakika boyunca. Daha sonra emme filtresinden ge\u00e7irerek s\u00fcpernatant s\u0131v\u0131 elde etmek i\u00e7in gerekli naringin enzim enzim s\u0131v\u0131s\u0131n\u0131 elde edin. Daha sonra kullanmak \u00fczere 4 \u2103'de so\u011futun.<\/h4>\r\n\r\n\r\n\r\n
\"\"<\/figure>\r\n\r\n\r\n\r\n

3. Kitosan \u0130mmobilize Naringinaz Enziminin Haz\u0131rlanmas\u0131<\/strong><\/h2>\r\n\r\n\r\n\r\n
\"\"<\/figure>\r\n\r\n\r\n\r\n

3.0 g kitosan, 100 mL hacim fraksiyonu 2% asetik asit \u00e7\u00f6zeltisi ile \u00e7\u00f6z\u00fclerek 0.3 g - L-1<\/sup>\u00a0Kitosan kolloidal \u00e7\u00f6zeltisi, hava kabarc\u0131klar\u0131n\u0131 gidermek i\u00e7in 20 dakika boyunca sonikasyona tabi tutulmu\u015f ve tamamen \u00e7\u00f6z\u00fcnmesine izin verilmi\u015ftir. Kitosan kolloidal \u00e7\u00f6zeltisi bir i\u011fne ile damla damla 0,5 g - L-1<\/sup>\u00a0D\u00fczg\u00fcn bir pelet olu\u015fturmak i\u00e7in NaOH kondensat\u0131 (1000 mL). Oda s\u0131cakl\u0131\u011f\u0131nda 3 saat bekletildi ve peletler n\u00f6tr hale gelene kadar tekrar tekrar dam\u0131t\u0131lm\u0131\u015f su ile y\u0131kand\u0131 ve kitosan mikrok\u00fcreleri elde etmek i\u00e7in su bir filtre ka\u011f\u0131d\u0131 taraf\u0131ndan emildi. 0,4 g kitosan mikrok\u00fcreler tart\u0131lm\u0131\u015f, 6% hacim fraksiyonlu 0,4 ml glutaraldehit eklenmi\u015f ve mikrok\u00fcreler, sabit s\u0131cakl\u0131k ve 25 \u2103 150 r-min titre\u015fimden sonra mikrok\u00fcrelerin y\u00fczeyinde glutaraldehit kalmay\u0131ncaya kadar distile su ile tekrar tekrar y\u0131kanm\u0131\u015ft\u0131r.-1<\/sup>\u00a0\u00c7apraz ba\u011fl\u0131 kitosan mikrok\u00fcreler filtre ka\u011f\u0131d\u0131 ile kurutularak elde edilmi\u015ftir.<\/h4>\r\n\r\n\r\n\r\n
\"\"<\/figure>\r\n\r\n\r\n\r\n

\u00a00,4 g kitosan mikrok\u00fcresi, 0,4 mL \u00fcye say\u0131s\u0131 6% glutaraldehit, 150 r - dk'da-1<\/sup>\u00a025 \u2103 s\u0131cakl\u0131k sal\u0131n\u0131m\u0131nda 2 saat sonra, mikro k\u00fcre y\u00fczeyine pentil Dialdehit olmayan dam\u0131t\u0131lm\u0131\u015f su ile tekrar tekrar y\u0131kan\u0131r ve \u00e7apraz ba\u011fl\u0131 kitosan mikrok\u00fcreleri elde etmek i\u00e7in filtre ka\u011f\u0131d\u0131 kurutulur. 4 mL naringinaz enzim \u00e7\u00f6zeltisi al\u0131n\u0131r, 0,4 g \u00e7apraz ba\u011fl\u0131 kitosan mikrok\u00fcrelere eklenir, 25 \u2103 sabit s\u0131cakl\u0131kta, 150 r-min'de \u00e7alkalan\u0131r-1<\/sup>\u00a0Ta\u015f\u0131y\u0131c\u0131n\u0131n y\u00fczeyinde kalan enzim \u00e7\u00f6zeltisini pH 4.5 tamponu ile durulay\u0131n, immobilize naringinaz elde etmek i\u00e7in filtre ka\u011f\u0131d\u0131ndaki suyu emdirin ve daha sonra kullanmak \u00fczere 4 \u2103'de buzdolab\u0131nda saklay\u0131n.<\/h4>\r\n\r\n\r\n\r\n
\"\"<\/figure>\r\n\r\n\r\n\r\n

4. Manyetik silikon bazl\u0131 kitosan (MSC) mikrok\u00fcrelerin haz\u0131rlanmas\u0131<\/strong><\/h2>\r\n\r\n\r\n\r\n
\"\"<\/figure>\r\n\r\n\r\n\r\n

Manyetik silikon bazl\u0131 nanopartik\u00fcllerin (MS) haz\u0131rlanma s\u00fcreci \u015eekil 1'de g\u00f6sterilmektedir.<\/h4>\r\n\r\n\r\n\r\n
\"\"<\/figure>\r\n\r\n\r\n\r\n

\u015eekil 1 Manyetik silikon bazl\u0131 nanopartik\u00fcllerin haz\u0131rlanmas\u0131n\u0131n \u015fematik diyagram\u0131<\/h4>\r\n\r\n\r\n\r\n
\"\"<\/figure>\r\n\r\n\r\n\r\n

( 1) \u0130lk olarak, manyetik Fe3<\/sub>O4<\/sub>\u00a0Sitrik asit ile modifiye edilmi\u015f nanopartik\u00fcller kimyasal birlikte \u00e7\u00f6kt\u00fcrme y\u00f6ntemi ile haz\u0131rlanm\u0131\u015ft\u0131r ve deneysel prosed\u00fcr \u015fu \u015fekildedir: 2.25 g FeCl3<\/sub>-6H2<\/sub>O ve 1,61 g FeSO4<\/sub>-7H2<\/sub>O, 100 mL deiyonize su ile doldurulmu\u015f \u00fc\u00e7 boyunlu balona eklenir, sistemin s\u0131cakl\u0131\u011f\u0131 azot gaz\u0131 ko\u015fullar\u0131nda 85 \u2103'ye \u0131s\u0131t\u0131l\u0131r. 1000 r-dk alt\u0131nda kar\u0131\u015ft\u0131r\u0131n-1<\/sup>\u00a0ve h\u0131zla 10 mL konsantre amonyakl\u0131 su d\u00f6k\u00fcn. \u00c7\u00f6zelti siyaha d\u00f6nd\u00fckten sonra 153 mg sodyum sitrat ekleyin. 30 dakikal\u0131k reaksiyondan sonra, \u00fcretimi bir m\u0131knat\u0131sla ay\u0131r\u0131n ve kat\u0131 faz\u0131 birka\u00e7 kez deiyonize su ve etanol ile y\u0131kay\u0131n. Fe3<\/sub>O4<\/sub>\u00a0Elde edilen nanopartik\u00fcller daha sonra kullan\u0131lmak \u00fczere kurutulur.<\/h4>\r\n\r\n\r\n\r\n
\"\"<\/figure>\r\n\r\n\r\n\r\n

(2) Ard\u0131ndan, \u015eekil 1'de g\u00f6sterilen sol-jel y\u00f6ntemiyle manyetik silikon bazl\u0131 nanopartik\u00fcller (MS) haz\u0131rlanm\u0131\u015ft\u0131r. Deneysel ad\u0131mlar \u015funlar\u0131 i\u00e7erir: 0.1 g Fe3<\/sub>O4<\/sub>\u00a0nanopartik\u00fcller, 40 mL susuz etanol, 10 mL dam\u0131t\u0131lm\u0131\u015f su ve 1,2 mL konsantre amonyakl\u0131 su i\u00e7eren kar\u0131\u015f\u0131k bir \u00e7\u00f6zelti i\u00e7ine homojen bir \u015fekilde da\u011f\u0131t\u0131lm\u0131\u015ft\u0131r. Kar\u0131\u015ft\u0131rma ko\u015fulu alt\u0131nda 300 r-min-1<\/sup>, Fe i\u00e7eren \u00e7\u00f6zelti damlac\u0131klar\u01313<\/sub>O4<\/sub>\u00a0nanopartik\u00fcller 30 mL susuz etanol i\u00e7inde da\u011f\u0131lm\u0131\u015f TEOS'a (0,4 mL) eklenmi\u015f ve silikon bazl\u0131 nanopartik\u00fcllerin reaksiyonu oda s\u0131cakl\u0131\u011f\u0131nda 12 saat boyunca ger\u00e7ekle\u015ftirilmi\u015ftir. \u00dcr\u00fcnler susuz etanol ve dam\u0131t\u0131lm\u0131\u015f su ile birka\u00e7 kez y\u0131kanarak manyetik silika bazl\u0131 nanopartik\u00fcller (MS) elde edilmi\u015f ve bunlar daha sonra kullan\u0131lmak \u00fczere kurutulmu\u015ftur.<\/h4>\r\n\r\n\r\n\r\n
\"\"<\/figure>\r\n\r\n\r\n\r\n

MSC mikrok\u00fcreleri \u015eekil 2'de g\u00f6sterildi\u011fi gibi em\u00fclsiyon \u00e7apraz ba\u011flama y\u00f6ntemiyle haz\u0131rlanm\u0131\u015ft\u0131r. Deneysel ad\u0131mlar \u015funlar\u0131 i\u00e7ermektedir: 0,125 g manyetik silika nanopartik\u00fcl (MS), hacim oran\u0131 5,0% olan asetik asit \u00e7\u00f6zeltisinde \u00e7\u00f6z\u00fcnd\u00fcr\u00fclen kitosan ile haz\u0131rlanan k\u00fctle oran\u0131 2,5% olan 10 mL kitosan \u00e7\u00f6zeltisine e\u015fit olarak da\u011f\u0131t\u0131lm\u0131\u015f ve ard\u0131ndan kitosan \u00e7\u00f6zeltisi 60 mL s\u0131v\u0131 parafin mumu ve 1,8 mL twain 80 i\u00e7eren bir em\u00fclsiyona damla damla eklenmi\u015ftir. 40 \u2103 su banyosu ve 800 dev\/dak ko\u015fullar\u0131nda 30 dakika kar\u0131\u015ft\u0131r\u0131ld\u0131ktan sonra-1<\/sup>2,0 mL glutaraldehit ekleyin ve 1 saat reaksiyona sokun. Daha sonra sistemin pH'\u0131 1 mol-L ile 9,0'a ayarland\u0131.-1<\/sup>\u00a0\u00dcr\u00fcnler petrol eteri, etanol ve dam\u0131t\u0131lm\u0131\u015f su ile birka\u00e7 kez y\u0131kanarak MSC mikrok\u00fcreleri elde edilmi\u015f ve bunlar daha sonra kullan\u0131lmak \u00fczere vakumda kurutulmu\u015ftur.<\/h4>\r\n\r\n\r\n\r\n
\"\"<\/figure>\r\n\r\n\r\n\r\n

\u015eekil 2 Manyetik silikon bazl\u0131 kitosan mikrok\u00fcrelerin haz\u0131rlanmas\u0131n\u0131n \u015fematik diyagram\u0131<\/h4>\r\n\r\n\r\n\r\n
\"\"<\/figure>\r\n\r\n\r\n\r\n

5. Epoksi bazl\u0131 manyetik silikon bazl\u0131 kitosan mikrok\u00fcrelerin (MSCE) haz\u0131rlanmas\u0131<\/strong><\/h2>\r\n\r\n\r\n\r\n
\"\"<\/figure>\r\n\r\n\r\n\r\n

4 y\u00f6ntemiyle haz\u0131rlanan kurutulmu\u015f MSC mikrok\u00fcrelerinden 0,5 g tart\u0131lm\u0131\u015f ve 50 mL'lik bir konik balona eklenmi\u015ftir. Ard\u0131ndan 2.0 mL NaOH \u00e7\u00f6zeltisi, epikrolohidrin \u00e7\u00f6zeltisi ve NaBH4<\/sub>\u00a0\u00e7\u00f6zeltisi s\u0131ras\u0131yla belirli bir konsantrasyonda \u015fi\u015feye eklenmi\u015ftir. Ve reaksiyon belirli bir s\u00fcre boyunca sabit s\u0131cakl\u0131k sal\u0131n\u0131m\u0131 ile ger\u00e7ekle\u015ftirilmi\u015ftir. MSCE'nin haz\u0131rlanmas\u0131na ili\u015fkin reaksiyon mekanizmas\u0131 \u015eekil 3'te g\u00f6sterilmektedir.<\/h4>\r\n\r\n\r\n\r\n
\"\"<\/figure>\r\n\r\n\r\n\r\n

\u015eekil 3 Epoksi bazl\u0131 manyetik silikon bazl\u0131 kitosan mikrok\u00fcrelerin haz\u0131rlanma prensibi<\/h4>\r\n\r\n\r\n\r\n
\"\"<\/figure>\r\n\r\n\r\n\r\n

Aktivasyondan sonra, epoksi bazl\u0131 manyetik silikon bazl\u0131 kitosan mikrok\u00fcreler (MSCE) elde etmek i\u00e7in y\u00fczeyde serbest OH- ve epoksi gruplar\u0131 kalmay\u0131ncaya kadar aktifle\u015ftirilmi\u015f mikrok\u00fcreler distile su ile tekrar tekrar y\u0131kan\u0131r. Tespit y\u00f6ntemi \u015fu \u015fekildedir: y\u0131kama s\u00fcpernatant\u0131ndan 3.0 mL al\u0131n\u0131r ve 1~2 damla fenolftalein eklenir. \u00c7alkalad\u0131ktan sonra, k\u0131rm\u0131z\u0131ya d\u00f6nmediyse, MSCE ile serbest OH- olmad\u0131\u011f\u0131 anlam\u0131na gelir; y\u0131kama s\u00fcpernatant\u0131ndan 3.0 mL al\u0131n ve 3.0 mL 1.3 mol-L-1<\/sup>\u00a0sodyum tiyos\u00fclfat ve 1 ~ 2 damla fenolftalein, \u00e7\u00f6zelti \u00e7alkaland\u0131ktan sonra k\u0131rm\u0131z\u0131ya d\u00f6nmediyse, MSCE ile serbest epoksi gruplar\u0131 olmad\u0131\u011f\u0131 anlam\u0131na gelir. Epoksi grubunun tespitine ili\u015fkin reaksiyon denklemi a\u015fa\u011f\u0131daki gibidir:<\/h4>\r\n\r\n\r\n\r\n
\"\"<\/figure>\r\n\r\n\r\n\r\n

6 MSCE immobilize naringinaz enziminin haz\u0131rlanmas\u0131<\/strong><\/h4>\r\n\r\n\r\n\r\n
\"\"<\/figure>\r\n\r\n\r\n\r\n

Y\u00f6ntem 5 ile haz\u0131rlanan 5.0 g MSCE, belirli bir pH de\u011ferine sahip 50 mL naringinaz enzim \u00e7\u00f6zeltisine eklenmi\u015f ve reaksiyon \u015eekil 4'te g\u00f6sterildi\u011fi gibi sabit s\u0131cakl\u0131ktaki bir su banyosu alt\u0131nda sal\u0131nm\u0131\u015ft\u0131r. Reaksiyondan sonra mikrok\u00fcreler, mikrok\u00fcrelerin y\u00fczeyinde serbest naringinaz bulunmayana kadar birka\u00e7 kez tampon \u00e7\u00f6zeltisi ile y\u0131kand\u0131. Mikrok\u00fcreler, daha sonra kullan\u0131lmak \u00fczere 4 \u00b0C'de so\u011futulan MSCE immobilize naringinaz enzimini elde etmek i\u00e7in bir Brinella hunisi ile bo\u015falt\u0131ld\u0131.\u00a0<\/h4>\r\n\r\n\r\n\r\n
\"\"<\/figure>\r\n\r\n\r\n\r\n

\u015eekil 4 MSCE immobilize naringinaz enziminin haz\u0131rlanma prensibi<\/h4>\r\n\r\n\r\n\r\n

\u015eimdi Bize Ula\u015f\u0131n!<\/b><\/strong><\/h2>\r\n

Fiyata ihtiyac\u0131n\u0131z varsa, l\u00fctfen a\u015fa\u011f\u0131daki forma ileti\u015fim bilgilerinizi doldurun, genellikle 24 saat i\u00e7inde sizinle ileti\u015fime ge\u00e7ece\u011fiz. Bana e-posta da g\u00f6nderebilirsiniz\u00a0info@longchangchemical.com<\/a><\/span>\u00a0\u00c7al\u0131\u015fma saatleri i\u00e7inde (8:30 - 6:00 UTC+8 Pzt.~Sat.) veya h\u0131zl\u0131 yan\u0131t almak i\u00e7in web sitesi canl\u0131 sohbetini kullan\u0131n.<\/b><\/strong><\/h4>\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n
Bile\u015fik Glukoamilaz<\/span><\/a><\/td>\r\n9032-08-0<\/td>\r\n<\/tr>\r\n
Pullulanase<\/span><\/a><\/td>\r\n9075-68-7<\/td>\r\n<\/tr>\r\n
Ksilanaz<\/span><\/a><\/td>\r\n37278-89-0<\/td>\r\n<\/tr>\r\n
Sel\u00fclaz<\/span><\/a><\/td>\r\n9012-54-8<\/td>\r\n<\/tr>\r\n
Naringinaz<\/span><\/a><\/td>\r\n9068-31-9<\/td>\r\n<\/tr>\r\n
\u03b2-Amilaz<\/span><\/a><\/td>\r\n9000-91-3<\/td>\r\n<\/tr>\r\n
Glikoz oksidaz<\/span><\/a><\/td>\r\n9001-37-0<\/td>\r\n<\/tr>\r\n
Alfa-Amilaz<\/td>\r\n9000-90-2<\/td>\r\n<\/tr>\r\n
Pektinaz<\/span><\/a><\/td>\r\n9032-75-1<\/td>\r\n<\/tr>\r\n
Peroksidaz<\/td>\r\n9003-99-0<\/td>\r\n<\/tr>\r\n
Lipaz<\/span><\/a><\/td>\r\n9001-62-1<\/td>\r\n<\/tr>\r\n
Katalaz<\/span><\/a><\/td>\r\n9001-05-2<\/td>\r\n<\/tr>\r\n
TANNASE<\/span><\/a><\/td>\r\n9025-71-2<\/td>\r\n<\/tr>\r\n
Elastaz<\/span><\/a><\/td>\r\n39445-21-1<\/td>\r\n<\/tr>\r\n
Urease<\/span><\/a><\/td>\r\n9002-13-5<\/td>\r\n<\/tr>\r\n
DEXTRANASE<\/span><\/a><\/td>\r\n9025-70-1<\/td>\r\n<\/tr>\r\n
L-Laktik dehidrojenaz<\/span><\/a><\/td>\r\n9001-60-9<\/td>\r\n<\/tr>\r\n
Dehidrojenaz malat<\/span><\/a><\/td>\r\n9001-64-3<\/td>\r\n<\/tr>\r\n
Kolesterol oksidaz<\/span><\/a><\/td>\r\n9028-76-6<\/td>\r\n<\/tr>\r\n<\/tbody>\r\n<\/table>","protected":false},"excerpt":{"rendered":"

1. Naringinaz enzim aktivitesinin tespit y\u00f6ntemi 1-1 Spektrofotometre y\u00f6ntemi Para nitrofenol spektrofotometrik y\u00f6ntemi, \u03b1-ramnosidaz ve \u03b2-glukozidaz\u0131n enzim aktivitesini tespit etmek i\u00e7in substrat olarak p-nitrofenol- \u03b1-ramnosid (pNP- \u03b1- Rha) ve p-nitrofenol-\u03b2-D-glukopiranosid (pNP- \u03b2-Glu) kullan\u0131r. Li Ping, enzimatik hidroliz i\u00e7in p-nitrofenol -\u03b2-glukozit (pNPG) \u00e7\u00f6zeltisine Aspergillus niger \u03b2-glukozidaz ekleme deneyinde p-nitrofenol spektrofotometre y\u00f6ntemini kulland\u0131 ve absorbans de\u011feri [...]'de \u00f6l\u00e7\u00fcld\u00fc.<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[108],"tags":[],"class_list":["post-4557","post","type-post","status-publish","format-standard","hentry","category-enzyme-news"],"yoast_head":"\nExperimental parameters of\u00a0naringinase immobilized on magnetic silicon based materials - Longchang Chemical<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/longchangchemical.com\/tr\/immobilized-on-magnetic-silicon-based-materials\/\" \/>\n<meta property=\"og:locale\" content=\"tr_TR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Experimental parameters of\u00a0naringinase immobilized on magnetic silicon based materials - Longchang Chemical\" \/>\n<meta property=\"og:description\" content=\"1.\u00a0Detection method of naringinase enzyme activity 1-1\u00a0Spectrophotometer method The para nitrophenol spectrophotometric method\u00a0uses p-nitrophenol-\u00a0\u03b1-rhamnoside (\u00a0pNP-\u00a0\u03b1-\u00a0Rha\u00a0) and p-nitrophenol-\u03b2-D-glucopyranoside (\u00a0pNP-\u00a0\u03b2-Glu) as substrate, for detecting the enzyme activity of\u00a0the \u03b1-rhamnosidase\u00a0and \u03b2-glucosidase.\u00a0Li Ping\u00a0used the p-nitrophenol spectrophotometer method, in the experiment of\u00a0adding\u00a0Aspergillus niger \u03b2-glucosidase into the solution of\u00a0p-nitrophenol\u00a0-\u03b2-glucoside (\u00a0pNPG\u00a0) for enzymatic hydrolysis, and the value of absorbance was measured at […]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/longchangchemical.com\/tr\/immobilized-on-magnetic-silicon-based-materials\/\" \/>\n<meta property=\"og:site_name\" content=\"Longchang Chemical\" \/>\n<meta property=\"article:published_time\" content=\"2021-04-29T10:10:14+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2024-09-28T11:56:32+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/longchangchemical.com\/wp-content\/uploads\/2021\/04\/image-45.png\" \/>\n<meta name=\"author\" content=\"Longchang Chemical\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\/\/longchangchemical.com\/immobilized-on-magnetic-silicon-based-materials\/#article\",\"isPartOf\":{\"@id\":\"https:\/\/longchangchemical.com\/immobilized-on-magnetic-silicon-based-materials\/\"},\"author\":{\"name\":\"Longchang Chemical\",\"@id\":\"https:\/\/longchangchemical.com\/#\/schema\/person\/ecb1ad846136c6f1ca534b62978c6a8e\"},\"headline\":\"Experimental parameters of\u00a0naringinase immobilized on magnetic silicon based materials\",\"datePublished\":\"2021-04-29T10:10:14+00:00\",\"dateModified\":\"2024-09-28T11:56:32+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\/\/longchangchemical.com\/immobilized-on-magnetic-silicon-based-materials\/\"},\"wordCount\":1840,\"commentCount\":0,\"publisher\":{\"@id\":\"https:\/\/longchangchemical.com\/#organization\"},\"image\":{\"@id\":\"https:\/\/longchangchemical.com\/immobilized-on-magnetic-silicon-based-materials\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/longchangchemical.com\/wp-content\/uploads\/2021\/04\/image-45.png\",\"articleSection\":[\"Enzyme News\"],\"inLanguage\":\"tr\",\"potentialAction\":[{\"@type\":\"CommentAction\",\"name\":\"Comment\",\"target\":[\"https:\/\/longchangchemical.com\/immobilized-on-magnetic-silicon-based-materials\/#respond\"]}]},{\"@type\":\"WebPage\",\"@id\":\"https:\/\/longchangchemical.com\/immobilized-on-magnetic-silicon-based-materials\/\",\"url\":\"https:\/\/longchangchemical.com\/immobilized-on-magnetic-silicon-based-materials\/\",\"name\":\"Experimental parameters of\u00a0naringinase immobilized on magnetic silicon based materials - 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Longchang Chemical","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/longchangchemical.com\/tr\/immobilized-on-magnetic-silicon-based-materials\/","og_locale":"tr_TR","og_type":"article","og_title":"Experimental parameters of\u00a0naringinase immobilized on magnetic silicon based materials - Longchang Chemical","og_description":"1.\u00a0Detection method of naringinase enzyme activity 1-1\u00a0Spectrophotometer method The para nitrophenol spectrophotometric method\u00a0uses p-nitrophenol-\u00a0\u03b1-rhamnoside (\u00a0pNP-\u00a0\u03b1-\u00a0Rha\u00a0) and p-nitrophenol-\u03b2-D-glucopyranoside (\u00a0pNP-\u00a0\u03b2-Glu) as substrate, for detecting the enzyme activity of\u00a0the \u03b1-rhamnosidase\u00a0and \u03b2-glucosidase.\u00a0Li Ping\u00a0used the p-nitrophenol spectrophotometer method, in the experiment of\u00a0adding\u00a0Aspergillus niger \u03b2-glucosidase into the solution of\u00a0p-nitrophenol\u00a0-\u03b2-glucoside (\u00a0pNPG\u00a0) for enzymatic hydrolysis, and the value of absorbance was measured at […]","og_url":"https:\/\/longchangchemical.com\/tr\/immobilized-on-magnetic-silicon-based-materials\/","og_site_name":"Longchang Chemical","article_published_time":"2021-04-29T10:10:14+00:00","article_modified_time":"2024-09-28T11:56:32+00:00","og_image":[{"url":"https:\/\/longchangchemical.com\/wp-content\/uploads\/2021\/04\/image-45.png","type":"","width":"","height":""}],"author":"Longchang Chemical","twitter_card":"summary_large_image","schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"Article","@id":"https:\/\/longchangchemical.com\/immobilized-on-magnetic-silicon-based-materials\/#article","isPartOf":{"@id":"https:\/\/longchangchemical.com\/immobilized-on-magnetic-silicon-based-materials\/"},"author":{"name":"Longchang Chemical","@id":"https:\/\/longchangchemical.com\/#\/schema\/person\/ecb1ad846136c6f1ca534b62978c6a8e"},"headline":"Experimental parameters of\u00a0naringinase immobilized on magnetic silicon based materials","datePublished":"2021-04-29T10:10:14+00:00","dateModified":"2024-09-28T11:56:32+00:00","mainEntityOfPage":{"@id":"https:\/\/longchangchemical.com\/immobilized-on-magnetic-silicon-based-materials\/"},"wordCount":1840,"commentCount":0,"publisher":{"@id":"https:\/\/longchangchemical.com\/#organization"},"image":{"@id":"https:\/\/longchangchemical.com\/immobilized-on-magnetic-silicon-based-materials\/#primaryimage"},"thumbnailUrl":"https:\/\/longchangchemical.com\/wp-content\/uploads\/2021\/04\/image-45.png","articleSection":["Enzyme News"],"inLanguage":"tr","potentialAction":[{"@type":"CommentAction","name":"Comment","target":["https:\/\/longchangchemical.com\/immobilized-on-magnetic-silicon-based-materials\/#respond"]}]},{"@type":"WebPage","@id":"https:\/\/longchangchemical.com\/immobilized-on-magnetic-silicon-based-materials\/","url":"https:\/\/longchangchemical.com\/immobilized-on-magnetic-silicon-based-materials\/","name":"Manyetik silikon bazl\u0131 malzemeler \u00fczerine immobilize edilmi\u015f naringinaz\u0131n deneysel parametreleri - 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