{"id":4434,"date":"2021-03-10T13:45:50","date_gmt":"2021-03-10T13:45:50","guid":{"rendered":"https:\/\/longchangchemical.com\/?p=4434"},"modified":"2024-09-28T11:54:34","modified_gmt":"2024-09-28T11:54:34","slug":"summary-of-different-glycoside-hydrolase-reactions","status":"publish","type":"post","link":"https:\/\/longchangchemical.com\/pt\/summary-of-different-glycoside-hydrolase-reactions\/","title":{"rendered":"Resumo das diferentes rea\u00e7\u00f5es da hidrolase de glicos\u00eddeo"},"content":{"rendered":"
Atualmente, as hidrolases de glicos\u00eddeos s\u00e3o usadas no estudo da prepara\u00e7\u00e3o de uma variedade de glicos\u00eddeos e agliconas ativos. Entre eles, a prepara\u00e7\u00e3o enzim\u00e1tica de glicos\u00eddeos de saponina e flavonoides em oxiglicos\u00eddeos \u00e9 a mais extensa. Ap\u00f3s anos de esfor\u00e7os de pesquisadores cient\u00edficos, a hidrolase de glicos\u00eddeo alcan\u00e7ou muitos resultados gratificantes na prepara\u00e7\u00e3o de glicos\u00eddeos e agliconas ativos.<\/h5>\r\n\r\n\r\n\r\n

1. Biotransforma\u00e7\u00e3o de glicos\u00eddeos flavonoides<\/h4>\r\n\r\n\r\n\r\n

Os flavonoides s\u00e3o polifen\u00f3is amplamente distribu\u00eddos nas plantas, principalmente na forma de glicos\u00eddeos. Estudos descobriram que os flavonoides com atividade biol\u00f3gica s\u00e3o os ingredientes ativos mais importantes das plantas comest\u00edveis e t\u00eam v\u00e1rias atividades farmacol\u00f3gicas, como prote\u00e7\u00e3o hep\u00e1tica, antioxida\u00e7\u00e3o, antitumoral e antiv\u00edrus, e sua atividade est\u00e1 intimamente relacionada \u00e0 estrutura. Como a maioria dos glicos\u00eddeos flavonoides \u00e9 dif\u00edcil de entrar no sangue atrav\u00e9s da parede do intestino delgado e sua biodisponibilidade \u00e9 baixa, a modifica\u00e7\u00e3o estrutural dos flavonoides naturais se tornou um ponto importante nas pesquisas atuais. O uso da hidrolase de glicos\u00eddeo para hidrolisar os grupos glicosil dos glicos\u00eddeos flavonoides tornou-se uma maneira eficaz de melhorar a atividade dos flavonoides (Tabela 1). Os glicos\u00eddeos flavonoides comuns incluem rutina, hesperidina e naringina, e suas por\u00e7\u00f5es de a\u00e7\u00facar s\u00e3o geralmente rutina (\u03b1-1,6 ramnose e glicose ligadas) e nova hesperose (\u03b1-1,2 ramnose e glicose ligadas), de modo que a hidrolase de glicos\u00eddeo hidrolisa a modifica\u00e7\u00e3o dela, incluindo principalmente dois tipos de exo- e endo-excis\u00e3o. A \u03b1-ramnosidase de Aspergillus niger e Aspergillus nidulans, que pode hidrolisar liga\u00e7\u00f5es \u03b1-1,2 e \u03b1-1,6 ramnos\u00eddeo, foi isolada e purificada. Essa enzima pode hidrolisar a rutina, a naringina e a hesperidina, produzindo, respectivamente, isoquercetina, plumos\u00eddeo e glicos\u00eddeo de hesperetina. A \u03b1-ramnosidase recombinante clonada de Aspergillus aculeatus e Clostridium stercorarium tamb\u00e9m tem a atividade de hidrolisar a ramnose em glicos\u00eddeos flavonoides. Para a hidr\u00f3lise dos tr\u00eas glicos\u00eddeos flavonoides acima, al\u00e9m das exoglicosidases, as endoglicosidases tamb\u00e9m t\u00eam um grande n\u00famero de relat\u00f3rios de pesquisa. A diglicosidase isolada e purificada de Penicillium rugulosum, Penicillium decumben[ e Fagopyri herba, bem como a rutinase recombinante clonada de Aspergillus niger, podem hidrolisar a rutina para produzir quercetina com melhor atividade antioxidante. A naringinase pode ser isolada e purificada do Aspergillus niger BCC 25166, que pode hidrolisar a naringina para produzir naringina. A naringinase do Aspergillus niger 1344 pode hidrolisar a naringina e a rutina ao mesmo tempo para produzir naringina, respectivamente. Yuan e quercetina, mas a hesperidina n\u00e3o pode ser hidrolisada. A diglicosidase em Acremonium sp. DSM24697 e Actinoplanes missouriensis pode hidrolisar a nova hesperidina na hesperidina para produzir produtos de hesperetina altamente ativos.<\/h5>\r\n\r\n\r\n\r\n

Tabela 1. Biotransforma\u00e7\u00e3o de glicos\u00eddeos flavonoides por glicosidase<\/h4>\r\n\r\n\r\n\r\n
\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n
Produto<\/td>\r\nSubstrato<\/td>\r\nRea\u00e7\u00e3o<\/td>\r\nOrganismo<\/td>\r\n<\/tr>\r\n
Isoquercitrina, Prunina,<\/td>\r\nRutina, Naringina,<\/td>\r\n\u03b1-Rhamnosidase<\/td>\r\nAspergillus niger<\/em><\/td>\r\n<\/tr>\r\n
Glucos\u00eddeo de hesperetina<\/td>\r\nHesperidina<\/td>\r\n\u03b1-Rhamnosidase<\/td>\r\nAspergillus nidulans<\/em><\/td>\r\n<\/tr>\r\n
\u00a0<\/td>\r\n\u00a0<\/td>\r\n\u03b1-Rhamnosidase<\/td>\r\nAspergillus aculeatus<\/em><\/td>\r\n<\/tr>\r\n
\u00a0<\/td>\r\n\u00a0<\/td>\r\n\u03b1-Rhamnosidase<\/td>\r\nClostridium stercorarium<\/em><\/td>\r\n<\/tr>\r\n
Quercetina<\/td>\r\nRutina<\/td>\r\n\u03b2-Rutinosidase<\/td>\r\nPenicillium rugulosum<\/em><\/td>\r\n<\/tr>\r\n
Quercetina<\/td>\r\nRutina<\/td>\r\n\u03b2-Glicosidase<\/td>\r\nPenicillium decumbens<\/em><\/td>\r\n<\/tr>\r\n
Quercetina<\/td>\r\nRutina<\/td>\r\n\u03b2-Heterodissacaridase<\/td>\r\nFagopyri herba<\/em><\/td>\r\n<\/tr>\r\n
Quercetina<\/td>\r\nRutina<\/td>\r\n\u03b2-Rutinosidase<\/td>\r\nAspergillus niger<\/em><\/td>\r\n<\/tr>\r\n
Naringenina<\/td>\r\nNaringina<\/td>\r\nNaringinase<\/td>\r\nAspergillus niger<\/em><\/td>\r\n<\/tr>\r\n
Naringenina, Quercetina<\/td>\r\nNaringina, Rutina<\/td>\r\nNaringinase<\/td>\r\nAspergillus niger<\/em><\/td>\r\n<\/tr>\r\n
Hesperetina<\/td>\r\nHesperidina<\/td>\r\nDiglicosidase<\/td>\r\nAcrem\u00f4nio<\/em><\/td>\r\n<\/tr>\r\n
Hesperetina<\/td>\r\nHesperidina<\/td>\r\nDiglicosidase<\/td>\r\nActinoplanes missouriensis<\/em><\/td>\r\n<\/tr>\r\n
Daidze\u00edna<\/td>\r\nDaidzin<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nMicr\u00f3bios n\u00e3o cultiv\u00e1veis<\/em><\/td>\r\n<\/tr>\r\n
Daidze\u00edna<\/td>\r\nDaidzin<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nSulfolobus solfataricus<\/em><\/td>\r\n<\/tr>\r\n
Daidze\u00edna<\/td>\r\nDaidzin<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nAspergillus oryzae<\/em><\/td>\r\n<\/tr>\r\n
Daidze\u00edna<\/td>\r\nDaidzin<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nPyrococcus furiosus<\/em><\/td>\r\n<\/tr>\r\n
Daidze\u00edna, Geniste\u00edna<\/td>\r\nDaidzin, Genistin<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nBacillus subtilis<\/em><\/td>\r\n<\/tr>\r\n
\u00a0<\/td>\r\n\u00a0<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nThermotoga maritima<\/em><\/td>\r\n<\/tr>\r\n
Daidze\u00edna, Geniste\u00edna,<\/td>\r\nDaidzin, Genistin,<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nDalbergia<\/em><\/td>\r\n<\/tr>\r\n
Glicite\u00edna<\/td>\r\nGlicitina<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nBacteroides thetaiotaomicron<\/em><\/td>\r\n<\/tr>\r\n
Baicale\u00edna<\/td>\r\nBaicalina<\/td>\r\n\u03b2-Glucuronidase<\/td>\r\nScutellaria viscidula<\/em><\/td>\r\n<\/tr>\r\n
Tilianin<\/td>\r\nLinarina<\/td>\r\nNaringinase<\/td>\r\nPenicillium decumbens<\/em><\/td>\r\n<\/tr>\r\n
Butin<\/td>\r\nButrin<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nAm\u00eandoa<\/em><\/td>\r\n<\/tr>\r\n
Cloretina<\/td>\r\nPhlorizin<\/td>\r\n\u03b2-Glicosidase<\/td>\r\nIntestino delgado de ovinos<\/em><\/td>\r\n<\/tr>\r\n<\/tbody>\r\n<\/table>\r\n<\/figure>\r\n\r\n\r\n\r\n
As isoflavonas s\u00e3o um tipo de flavon\u00f3ides, encontrados principalmente em leguminosas, que contribuem para a preven\u00e7\u00e3o de doen\u00e7as e para a sa\u00fade humana. Os principais componentes das isoflavonas da soja s\u00e3o daidze\u00edna, daidze\u00edna, genistina, geniste\u00edna, glicite\u00edna e glicite\u00edna aglicona, entre as quais a aglicona desglicosilada tem melhor atividade biol\u00f3gica. A \u03b2-glucosidase recombinante capaz de hidrolisar a daidze\u00edna para produzir daidze\u00edna foi clonada da biblioteca de genes do solo de mangue, Sulfolobus solfataricus, Aspergillus oryzae e Pyrococcus furiosus; a \u03b2-glucosidase recombinante clonada das enzimas Thermotoga maritima e Bacillus subtilis pode hidrolisar a daidze\u00edna e a geniste\u00edna para produzir daidze\u00edna e geniste\u00edna; a glicosidase isolada e purificada de Dalbergia e clonada e recombinada de Bacteroides thetaiotaomicron pode hidrolisar daidze\u00edna, genistina e daidze\u00edna para produzir daidze\u00edna, geniste\u00edna e glicite\u00edna aglicona.<\/h5>\r\n\r\n\r\n\r\n
A hidrolase de glicos\u00eddeo tamb\u00e9m foi relatada e aplicada na hidr\u00f3lise de outros glicos\u00eddeos flavonoides (Tabela 1). Estudos demonstraram que a baicalina tem efeitos antitumorais e anti-infecciosos. A \u03b2-glucosidase isolada e purificada da Scutellaria viscidula Bge pode hidrolisar a baicalina para produzir baicale\u00edna. O produto desglicosilado, a baicale\u00edna, tem melhor atividade farmacol\u00f3gica. A serimarina tamb\u00e9m \u00e9 um glicos\u00eddeo flavonoide raro com atividades anti-hipertensivas e sedativas, mas \u00e9 dif\u00edcil de obter por extra\u00e7\u00e3o direta e s\u00edntese qu\u00edmica. Cui et al. usaram a naringinase para hidrolisar a ramnose no montanos\u00eddeo para produzir a serimarina. Al\u00e9m disso, Jassbi et al. usaram a \u03b2-glucosidase para hidrolisar a butrina e produzir butrina. Os resultados dos experimentos antioxidantes mostraram que a butrina desglicosilada tinha melhor atividade do que a butrina. Day e outras glicosidases isoladas e purificadas do intestino delgado de ovelhas podem hidrolisar a clorizina para produzir floretina.<\/h5>\r\n\r\n\r\n\r\n

2. Biotransforma\u00e7\u00e3o de outros oxiglicos\u00eddeos<\/h4>\r\n\r\n\r\n\r\n

Al\u00e9m das saponinas e dos glicos\u00eddeos flavonoides, as hidrolases de glicos\u00eddeos tamb\u00e9m foram usadas para hidrolisar e modificar outros glicos\u00eddeos de oxig\u00eanio (Tabela 2). A fruta gard\u00eania \u00e9 um medicamento tradicional chin\u00eas usado para tratar doen\u00e7as cardiovasculares, cerebrovasculares, do f\u00edgado e da ves\u00edcula biliar. H\u00e1 uma grande quantidade de genipos\u00eddeo nas frutas de gard\u00eania, mas o ingrediente eficaz \u00e9 o genipos\u00eddeo, que \u00e9 o produto desglicosilado do genipos\u00eddeo, e o conte\u00fado \u00e9 inferior a 0,01%. A \u03b2-glucosidase isolada e purificada de Penicillium nigricans e Aspergillus niger pode ser transformada em genipos\u00eddeo para preparar genipin para atender \u00e0 demanda por grandes quantidades de genipin. A Arctium lappa tem o efeito de prevenir ou tratar a insufici\u00eancia renal cr\u00f4nica, e seus ingredientes eficazes s\u00e3o a arctiina e a arctigenina. A \u03b2-glucosidase em Grifola frondosa e Rhizoctonia solani pode transformar a bardana para produzir arctigenina. Liu et al. usaram a \u03b2-glucosidase comercial para hidrolisar completamente o fruto da bardana e obter o produto aglicona da bardana. A convers\u00e3o da arctiina em arctigenina pode melhorar efetivamente a biodisponibilidade. O resveratrol tem a fun\u00e7\u00e3o de prevenir tumores e aterosclerose. A \u03b2-glucosidase isolada e purificada de Aspergillus oryzae sp. 100 e Lactobacillus kimchi e a \u03b2-glucosidase recombinante clonada a partir da metagen\u00f4mica do solo de mangue por Mai et al. A glucosidase pode converter a polidatina para produzir resveratrol. O paclitaxel \u00e9 um metab\u00f3lito secund\u00e1rio da planta Taxus chinensis e tem um bom efeito terap\u00eautico no c\u00e2ncer de ov\u00e1rio e no c\u00e2ncer de mama. O peso seco do paclitaxel no Taxus chinensis \u00e9 de apenas 0,02%, e o conte\u00fado de 7-xilose-10-desacetilpaclitaxel descartado como res\u00edduo \u00e9 mais de 10 vezes maior que o do paclitaxel. Dou et al. usaram a xilosidase extracelular secretada pela cepa F16 do Cellulosimicrobium cellulans para converter o 7-xilose-10-desacetilpaclitaxel em 10-desacetilpaclitaxel e, em seguida, por meio de uma rea\u00e7\u00e3o de acila\u00e7\u00e3o em uma etapa para gerar o paclitaxel.<\/h5>\r\n\r\n\r\n\r\n

Tabela 2. Biotransforma\u00e7\u00e3o de outros O-glicos\u00eddeos pela glicosidase<\/h4>\r\n\r\n\r\n\r\n
\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n
Produto<\/td>\r\nSubstrato<\/td>\r\nRea\u00e7\u00e3o<\/td>\r\nOrganismo<\/td>\r\n<\/tr>\r\n
Genipin<\/td>\r\nGenipos\u00eddeo<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nPenicillium<\/em>\u00a0<\/em>nigricans<\/em><\/td>\r\n<\/tr>\r\n
Genipin<\/td>\r\nGenipos\u00eddeo<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nAspergillus<\/em>\u00a0<\/em>N\u00edger<\/em><\/td>\r\n<\/tr>\r\n
Arctigenina<\/td>\r\nArctiin<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nGrifola<\/em>\u00a0<\/em>frondosa<\/em><\/td>\r\n<\/tr>\r\n
Arctigenina<\/td>\r\nArctiin<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nRhizoctonia<\/em>\u00a0<\/em>solani<\/em><\/td>\r\n<\/tr>\r\n
Arctigenina<\/td>\r\nArctiin<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nComercial<\/td>\r\n<\/tr>\r\n
Resveratrol<\/td>\r\nPolidatina<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nAspergillus<\/em>\u00a0<\/em>oryzae<\/em><\/td>\r\n<\/tr>\r\n
Resveratrol<\/td>\r\nPolidatina<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nLactobacilos<\/em>\u00a0<\/em>kimchi<\/em><\/td>\r\n<\/tr>\r\n
Resveratrol<\/td>\r\nPolidatina<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nMicr\u00f3bios n\u00e3o cultiv\u00e1veis<\/td>\r\n<\/tr>\r\n
10-Deacetilpaclitaxel<\/td>\r\n7-Xilosil-10-deacetilpaclitaxel<\/td>\r\n\u03b2-xilosidase<\/td>\r\nCellulosimicrobium<\/em>\u00a0<\/em>celulano<\/em>s<\/td>\r\n<\/tr>\r\n<\/tbody>\r\n<\/table>\r\n<\/figure>\r\n\r\n\r\n\r\n

3. Biotransforma\u00e7\u00e3o de glicos\u00eddeos de carbono e tioglicos\u00eddeos<\/h4>\r\n\r\n\r\n\r\n

Al\u00e9m dos oxiglicos\u00eddeos, as hidrolases de glicos\u00eddeos tamb\u00e9m s\u00e3o usadas no estudo da modifica\u00e7\u00e3o da hidr\u00f3lise de glicos\u00eddeos de carbono e tioglicos\u00eddeos (Tabela 3). Os glicos\u00eddeos de carbono s\u00e3o formados pela condensa\u00e7\u00e3o da desidrata\u00e7\u00e3o do hidrog\u00eanio da posi\u00e7\u00e3o orto ou para ativada pelo grupo hidroxila fen\u00f3lico do aglicona com grupos de a\u00e7\u00facar. Os flavonoides carbos\u00eddeos t\u00eam muitas atividades, como anti-inflamat\u00f3ria, antibacteriana, antitumoral, redu\u00e7\u00e3o do a\u00e7\u00facar no sangue e aumento da imunidade. Em compara\u00e7\u00e3o com os flavonoides oxos\u00eddeos, os flavonoides carbos\u00eddeos t\u00eam maior estabilidade e podem ser completamente absorvidos e se tornar mol\u00e9culas de medicamentos em potencial. Como as liga\u00e7\u00f5es glicos\u00eddicas de carbono s\u00e3o dif\u00edceis de serem hidrolisadas, h\u00e1 poucos relatos sobre a hidr\u00f3lise de flavonoides de glicos\u00eddeo de carbono. Sanugul et al. isolaram uma bact\u00e9ria de uma mistura de bact\u00e9rias fecais humanas, que secretou uma glicosidase sob a indu\u00e7\u00e3o de mangiferina, que pode hidrolisar as liga\u00e7\u00f5es glicos\u00eddicas de carbono na mangiferina para produzir mangiferina com melhor atividade. Nakamura et al. isolaram a cepa PUE da bact\u00e9ria intestinal humana, que pode isolar e purificar uma carboglicosidase que hidrolisa a puerarina para produzir aglicona. No estudo de genes codificadores de carboglicosidase, Braune et al. descobriram que os genes codificadores de prote\u00edna dfgA, dfgB, dfgC, dfgD e dfgE em Eubacterium cellulosolvens co-expressam carboglicosidases que podem hidrolisar a isoorientina para produzir os agliconas correspondentes.<\/h5>\r\n\r\n\r\n\r\n
Os glucosinolatos s\u00e3o uma importante classe de compostos glucosinolatos, amplamente encontrados em vegetais cruc\u00edferos, como mostarda, br\u00f3colis, alho e outros. Estudos demonstraram que o consumo de vegetais cruc\u00edferos pode prevenir com efic\u00e1cia o c\u00e2ncer de mama, o c\u00e2ncer de pulm\u00e3o, o c\u00e2ncer de c\u00f3lon e outros tipos de c\u00e2ncer. O principal ingrediente ativo \u00e9 o isotiocianato produzido ap\u00f3s a degrada\u00e7\u00e3o do glucosinolato. A glucosidase, tamb\u00e9m conhecida como mirosinase, \u00e9 encontrada principalmente em vegetais cruc\u00edferos, mas \u00e9 distribu\u00edda em uma posi\u00e7\u00e3o diferente dos glucosinolatos. Somente quando as c\u00e9lulas forem rompidas, elas se misturar\u00e3o e reagir\u00e3o. Devido ao baixo teor de mirosinase end\u00f3gena, \u00e9 dif\u00edcil hidrolisar efetivamente os glucosinolatos para produzir produtos ativos. O sulforafano \u00e9 um isotiocianato com atividade farmacol\u00f3gica. Shen et al. usaram a mirosinase ex\u00f3gena para converter com sucesso a glucorafanina em sulforafano. No momento, h\u00e1 poucos estudos sobre a modifica\u00e7\u00e3o hidrol\u00edtica de glicos\u00eddeos de carbono e tioglicos\u00eddeos. No futuro, o desenvolvimento e a modifica\u00e7\u00e3o estrutural de mais glicos\u00eddeos de carbono e tioglicos\u00eddeos fornecer\u00e3o mais mol\u00e9culas candidatas ao desenvolvimento de medicamentos.<\/h5>\r\n\r\n\r\n\r\n

Tabela 3. Biotransforma\u00e7\u00e3o de C-glicos\u00eddeos e S-glicos\u00eddeos pela glicosidase.<\/h4>\r\n\r\n\r\n\r\n
\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n
Produto<\/td>\r\nSubstrato<\/td>\r\nRea\u00e7\u00e3o<\/td>\r\nOrganismo<\/td>\r\n<\/tr>\r\n
Noratriol<\/td>\r\nMangiferina<\/td>\r\nEnzima de quebra de C-glucosil<\/td>\r\nBacteroides<\/em><\/td>\r\n<\/tr>\r\n
Daidze\u00edna<\/td>\r\nPuerarina<\/td>\r\nEnzima de quebra de C-glucosil<\/td>\r\nHumanos<\/em>\u00a0<\/em>intestinal<\/em>\u00a0<\/em>bact\u00e9ria<\/em><\/td>\r\n<\/tr>\r\n
Luteolina<\/td>\r\nHomoorienta\u00e7\u00e3o<\/td>\r\nEnzima de quebra de C-glucosil<\/td>\r\nEubact\u00e9ria<\/em>\u00a0<\/em>cellulosolvens<\/em><\/td>\r\n<\/tr>\r\n
Sulforafano<\/td>\r\nGlucorafanina<\/td>\r\nMirosinase<\/td>\r\nSementes de br\u00f3colis<\/em><\/td>\r\n<\/tr>\r\n<\/tbody>\r\n<\/table>\r\n<\/figure>\r\n\r\n\r\n\r\n

Entre em contato conosco agora!<\/b><\/strong><\/h2>\r\n

Se precisar do Price, preencha suas informa\u00e7\u00f5es de contato no formul\u00e1rio abaixo. Normalmente, entraremos em contato dentro de 24 horas. Voc\u00ea tamb\u00e9m pode me enviar um e-mail\u00a0info@longchangchemical.com<\/a><\/span>\u00a0durante o hor\u00e1rio comercial (das 8h30 \u00e0s 18h UTC+8 de segunda a s\u00e1bado) ou use o bate-papo ao vivo do site para obter uma resposta imediata.<\/b><\/strong><\/h4>\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n
Composto Glucoamilase<\/span><\/a><\/td>\r\n9032-08-0<\/td>\r\n<\/tr>\r\n
Pullulanase<\/span><\/a><\/td>\r\n9075-68-7<\/td>\r\n<\/tr>\r\n
Xilanase<\/span><\/a><\/td>\r\n37278-89-0<\/td>\r\n<\/tr>\r\n
Celulase<\/span><\/a><\/td>\r\n9012-54-8<\/td>\r\n<\/tr>\r\n
Naringinase<\/span><\/a><\/td>\r\n9068-31-9<\/td>\r\n<\/tr>\r\n
\u03b2-Amilase<\/span><\/a><\/td>\r\n9000-91-3<\/td>\r\n<\/tr>\r\n
Glucose oxidase<\/span><\/a><\/td>\r\n9001-37-0<\/td>\r\n<\/tr>\r\n
alfa-Amilase<\/td>\r\n9000-90-2<\/td>\r\n<\/tr>\r\n
Pectinase<\/span><\/a><\/td>\r\n9032-75-1<\/td>\r\n<\/tr>\r\n
Peroxidase<\/td>\r\n9003-99-0<\/td>\r\n<\/tr>\r\n
Lipase<\/span><\/a><\/td>\r\n9001-62-1<\/td>\r\n<\/tr>\r\n
Catalase<\/span><\/a><\/td>\r\n9001-05-2<\/td>\r\n<\/tr>\r\n
TANNASE<\/span><\/a><\/td>\r\n9025-71-2<\/td>\r\n<\/tr>\r\n
Elastase<\/span><\/a><\/td>\r\n39445-21-1<\/td>\r\n<\/tr>\r\n
Urease<\/span><\/a><\/td>\r\n9002-13-5<\/td>\r\n<\/tr>\r\n
DEXTRANASE<\/span><\/a><\/td>\r\n9025-70-1<\/td>\r\n<\/tr>\r\n
L-L\u00e1ctico desidrogenase<\/span><\/a><\/td>\r\n9001-60-9<\/td>\r\n<\/tr>\r\n
Malato desidrogenase<\/span><\/a><\/td>\r\n9001-64-3<\/td>\r\n<\/tr>\r\n
Colesterol oxidase<\/span><\/a><\/td>\r\n9028-76-6<\/td>\r\n<\/tr>\r\n<\/tbody>\r\n<\/table>","protected":false},"excerpt":{"rendered":"

Atualmente, as hidrolases de glicos\u00eddeos s\u00e3o usadas no estudo da prepara\u00e7\u00e3o de uma variedade de glicos\u00eddeos e agliconas ativos. Entre eles, a prepara\u00e7\u00e3o enzim\u00e1tica de glicos\u00eddeos de saponina e flavonoides em oxiglicos\u00eddeos \u00e9 a mais extensa. Ap\u00f3s anos de esfor\u00e7os de pesquisadores cient\u00edficos, a hidrolase de glicos\u00eddeo alcan\u00e7ou muitos resultados gratificantes na prepara\u00e7\u00e3o de glicos\u00eddeos ativos [...]<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[108],"tags":[],"class_list":["post-4434","post","type-post","status-publish","format-standard","hentry","category-enzyme-news"],"yoast_head":"\nSummary of different glycoside hydrolase reactions - Longchang Chemical Co., Ltd.<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/longchangchemical.com\/pt\/summary-of-different-glycoside-hydrolase-reactions\/\" \/>\n<meta property=\"og:locale\" content=\"pt_BR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Summary of different glycoside hydrolase reactions - Longchang Chemical Co., Ltd.\" \/>\n<meta property=\"og:description\" content=\"At present, glycoside hydrolases are used in the study of the preparation of a variety of active glycosides and aglycons. 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