{"id":4557,"date":"2021-04-29T10:10:14","date_gmt":"2021-04-29T10:10:14","guid":{"rendered":"https:\/\/longchangchemical.com\/?p=4557"},"modified":"2024-09-28T11:56:32","modified_gmt":"2024-09-28T11:56:32","slug":"immobilized-on-magnetic-silicon-based-materials","status":"publish","type":"post","link":"https:\/\/longchangchemical.com\/hu\/immobilized-on-magnetic-silicon-based-materials\/","title":{"rendered":"M\u00e1gneses szil\u00edcium alap\u00fa anyagokon immobiliz\u00e1lt naringin\u00e1z k\u00eds\u00e9rleti param\u00e9terei"},"content":{"rendered":"

1. A naringin\u00e1z enzimaktivit\u00e1s kimutat\u00e1si m\u00f3dszere<\/strong><\/h1>\r\n\r\n\r\n\r\n
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1-1 Spektrofotom\u00e9teres m\u00f3dszer<\/h3>\r\n\r\n\r\n\r\n
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A para-nitrofenol spektrofotometri\u00e1s m\u00f3dszer p-nitrofenol- \u03b1-ramnosidot ( pNP- \u03b1- Rha ) \u00e9s p-nitrofenol-\u03b2-D-gl\u00fckopiranozidot ( pNP- \u03b2-Glu ) haszn\u00e1l szubsztr\u00e1tk\u00e9nt az \u03b1-ramnosid\u00e1z \u00e9s \u03b2-gl\u00fckozid\u00e1z enzimaktivit\u00e1s\u00e1nak kimutat\u00e1s\u00e1ra. Li Ping a p-nitrofenol spektrofotom\u00e9teres m\u00f3dszert alkalmazta, a k\u00eds\u00e9rletben Aspergillus niger \u03b2-gl\u00fckozid\u00e1zt adtak a p-nitrofenol -\u03b2-gl\u00fckozid ( pNPG ) oldat\u00e1hoz enzimatikus hidrol\u00edzis c\u00e9lj\u00e1b\u00f3l, \u00e9s az abszorbancia \u00e9rt\u00e9k\u00e9t 400 nm ultraibolya hull\u00e1mhosszon m\u00e9rt\u00e9k. Az abszorbancia \u00e9rt\u00e9ke alapj\u00e1n pedig kisz\u00e1m\u00edtott\u00e1k a \u03b2 -gl\u00fckozid\u00e1z enzimaktivit\u00e1s\u00e1t. Ez a m\u00f3dszer er\u0151s szubsztr\u00e1tspecifit\u00e1ssal rendelkezik, de a standard szubsztr\u00e1t dr\u00e1ga, \u00e9s a naringin\u00e1z enzimaktivit\u00e1sa az \u00e9lelmiszerekben l\u00e9v\u0151 glikozidok hidroliz\u00e1l\u00e1s\u00e1ra nem hat\u00e1rozhat\u00f3 meg, ez\u00e9rt ez a m\u00f3dszer nem alkalmas az enzimaktivit\u00e1s kimutat\u00e1s\u00e1ra a naringin\u00e1z ipari nagy\u00fczemi el\u0151\u00e1ll\u00edt\u00e1s\u00e1ban.<\/h4>\r\n\r\n\r\n\r\n
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A Davis spektrofotom\u00e9teres m\u00f3dszer elve az, hogy a naringin enyh\u00e9n l\u00fagos k\u00f6r\u00fclm\u00e9nyek k\u00f6z\u00f6tt kromog\u00e9n reakci\u00f3ba l\u00e9phet a 90% dietil\u00e9nglikollal, \u00e9s a s\u00e1rga naringin-kalkon termel\u0151d\u00e9se 420 nm hull\u00e1mhossz\u00fa ultraibolya f\u00e9nyben kimutathat\u00f3. A m\u00e9rt abszorbancia\u00e9rt\u00e9k alapj\u00e1n kisz\u00e1m\u00edthat\u00f3 a szubsztr\u00e1t naringin marad\u00e9k mennyis\u00e9ge, majd sz\u00e1m\u00edt\u00e1ssal megkaphat\u00f3 a naringin\u00e1z aktivit\u00e1s. Wang Weina \u00e9s munkat\u00e1rsai egy tov\u00e1bbfejlesztett Davis-m\u00f3dszert alkalmaztak, szubsztr\u00e1tk\u00e9nt naringint haszn\u00e1ltak, \u00e9s az Aspergillus niger FFCC 848 foly\u00e9kony ferment\u00e1ci\u00f3val el\u0151\u00e1ll\u00edtott naringin\u00e1zt adtak hozz\u00e1 az enzimatikus hidrol\u00edzishez. Az abszorbanci\u00e1t 420 nm-es ultraibolya f\u00e9nyben m\u00e9rt\u00e9k, majd kisz\u00e1m\u00edtott\u00e1k az enzimaktivit\u00e1st. Az enzimaktivit\u00e1s m\u00e9r\u00e9s\u00e9re szolg\u00e1l\u00f3 Davis spektrofotom\u00e9teres m\u00f3dszer egyszer\u0171 a m\u0171k\u00f6d\u00e9shez, gyors sebess\u00e9ggel rendelkezik, \u00edgy ezt a m\u00f3dszert sz\u00e9les k\u00f6rben haszn\u00e1lj\u00e1k a ferment\u00e1ci\u00f3val termelt naringin\u00e1z \u00e9s az immobiliz\u00e1lt naringin\u00e1z aktivit\u00e1s\u00e1nak eeal time kimutat\u00e1s\u00e1ra.<\/h4>\r\n\r\n\r\n\r\n
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1-2 Nagy teljes\u00edtm\u00e9ny\u0171 folyad\u00e9kkromatogr\u00e1fia<\/h3>\r\n\r\n\r\n\r\n
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A nagy teljes\u00edtm\u00e9ny\u0171 folyad\u00e9kkromatogr\u00e1fia ( HPLC) elve az, hogy a HPLC k\u00e9pes pontosan meghat\u00e1rozni \u00e9s mennyis\u00e9gileg elemezni a szubsztr\u00e1t naringint, a k\u00f6ztes term\u00e9k purunint \u00e9s a v\u00e9gterm\u00e9k naringenint a teljes hidrol\u00edzisfolyamat sor\u00e1n, majd kisz\u00e1m\u00edtani az enzimaktivit\u00e1st. Chen Yuelong \u00e9s m\u00e1sok HPLC-t haszn\u00e1ltak a naringin, az arbutin, a miricetin \u00e9s term\u00e9keik retenci\u00f3s idej\u00e9nek meghat\u00e1roz\u00e1s\u00e1ra, \u00e9s teljes elv\u00e1laszt\u00e1st \u00e9rtek el 1,5-n\u00e9l nagyobb felbont\u00e1ssal, \u00e9s hat\u00e9konyan nyomon k\u00f6vett\u00e9k a naringin\u00e1z hat\u00e1s\u00e1t t\u00f6bb glikozidra a hidrol\u00edzisfolyamatban, ugyanakkor az enzimaktivit\u00e1st pontosan meghat\u00e1rozt\u00e1k. A HPLC-m\u00f3dszer el\u0151nye a nagy pontoss\u00e1g \u00e9s a szennyez\u0151d\u00e9sek interferenci\u00e1j\u00e1nak elker\u00fcl\u00e9se, de a Davis-m\u00f3dszerrel \u00f6sszehasonl\u00edtva ez a m\u00f3dszer hosszabb detekt\u00e1l\u00e1si id\u0151vel \u00e9s bonyolultabb m\u0171velettel rendelkezik.<\/h4>\r\n\r\n\r\n\r\n
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1-3 Jav\u00edtott m\u00f3dszer a naringin\u00e1z enzimaktivit\u00e1s meghat\u00e1roz\u00e1s\u00e1ra<\/h3>\r\n\r\n\r\n\r\n
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A tov\u00e1bbfejlesztett Davis-m\u00f3dszer alkalmaz\u00e1s\u00e1val: 0,8 ml 0,8 g-L-1<\/sup>\u00a0A 40 mg immobiliz\u00e1lt naringin\u00e1zt (vagy 0,2 ml szabad naringin\u00e1zt) tartalmaz\u00f3 reakci\u00f3rendszerbe pH 4,5 pufferrel k\u00e9sz\u00edtett naringinin oldatot adtunk. Helyezz\u00fck 50 \u2103-os \u00e1lland\u00f3 h\u0151m\u00e9rs\u00e9klet\u0171 v\u00edzf\u00fcrd\u0151be 30 percre. Vegy\u00fcnk 0,1 mL-t a reakci\u00f3 enzimes hidrol\u00edzis oldat\u00e1b\u00f3l, \u00e9s adjunk hozz\u00e1 5 mL dietil\u00e9nglikolt (90% t\u00e9rfogatfrakci\u00f3) \u00e9s 0,1 mL NaOH oldatot (4 mol-L-1<\/sup>) a cs\u00f6vekbe, \u00e9s 10 percig \u00e1llni hagyjuk, miut\u00e1n egyenletesen \u00f6sszekevert\u00fck \u0151ket. M\u00e9rj\u00fck meg a kevert oldat abszorbanci\u00e1j\u00e1t 420 nm-en.<\/h4>\r\n\r\n\r\n\r\n
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A naringin\u00e1z enzim aktivit\u00e1s\u00e1nak meghat\u00e1roz\u00e1sa (U) : pH 4,5, 50 \u2103 mellett 1 enzimaktivit\u00e1si egys\u00e9g az az enzimmennyis\u00e9g, amely 1 \u03bcg naringin percenk\u00e9nti lebont\u00e1s\u00e1hoz sz\u00fcks\u00e9ges. Az enzimaktivit\u00e1s ar\u00e1nya ( U-g-1<\/sup>\u00a0vagy U-mL-1<\/sup>) meghat\u00e1roz\u00e1sa: pH 4,5, 50 \u2103, 1 g immobiliz\u00e1lt naringin\u00e1z (vagy 1 ml szabad naringin\u00e1z) \u00e1ltal mutatott enzimaktivit\u00e1s. A relat\u00edv enzimaktivit\u00e1s kisz\u00e1m\u00edt\u00e1sa a k\u00f6vetkez\u0151 k\u00e9plet szerint t\u00f6rt\u00e9nik :<\/h4>\r\n\r\n\r\n\r\n
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Relat\u00edv enzimaktivit\u00e1s \/% = enzimaktivit\u00e1s\/maxim\u00e1lis enzimaktivit\u00e1s \u00d7 100<\/h4>\r\n\r\n\r\n\r\n
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A standard g\u00f6rbe meghat\u00e1roz\u00e1sa: a standard glikozid naringin oldat t\u00f6megkoncentr\u00e1ci\u00f3ja 0, 0,4, 0,8, 1,2, 1,6 g - L-1<\/sup>, majd 0,1 mL oldatot vett\u00fcnk 5 mL dietil\u00e9nglikollal (90% , v\/v) \u00e9s 0,1 mL n\u00e1trium-hidroxid-oldattal ( 4 mol-L-1<\/sup>). Miut\u00e1n az elegyet egyenletesen \u00f6sszekevert\u00fck \u00e9s 10 percig \u00e1llni hagytuk, a kevert oldat f\u00e9nyelnyel\u00e9si \u00e9rt\u00e9k\u00e9t 420 nm-en megm\u00e9rt\u00fck, \u00e9s ennek megfelel\u0151en megrajzoltuk a standard g\u00f6rb\u00e9t.<\/h4>\r\n\r\n\r\n\r\n
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2. A naringin\u00e1z enzimoldat elk\u00e9sz\u00edt\u00e9se<\/strong><\/h2>\r\n\r\n\r\n\r\n
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A 4 \u2103-on t\u00e1rolt Aspergillus niger FFCC uv-11-et \u00e1tvitt\u00fck a ferde t\u00e1ptalajra, \u00e9s az \u00e9rett sp\u00f3r\u00e1kat \u00e1lland\u00f3 h\u0151m\u00e9rs\u00e9kleten, 30 \u2103-on 96 \u00f3r\u00e1n \u00e1t t\u00f6rt\u00e9n\u0151 teny\u00e9szt\u00e9ssel nyert\u00fck. Ezut\u00e1n a sp\u00f3r\u00e1kat steril norm\u00e1l s\u00f3oldattal mostuk 0,9% t\u00f6megfrakci\u00f3val. 600 nm-en m\u00e9rt\u00fck a sp\u00f3raszuszpenzi\u00f3 abszorbancia \u00e9rt\u00e9k\u00e9t (OD600 \u00e9rt\u00e9k), steril s\u00f3oldattal az OD600 \u00e9rt\u00e9k\u00e9t 0,200-ra \u00e1ll\u00edtottuk be. \u00c9s ezut\u00e1n a sp\u00f3raszuszpenzi\u00f3 t\u00e9rfogatfrakci\u00f3ja 10% beolt\u00e1si mennyis\u00e9g 100 ml ferment\u00e1ci\u00f3s k\u00f6zeghez (250 ml-es k\u00fapos lombik), A kapott ferment\u00e1ci\u00f3s levet 4 \u2103 \u00e9s 6000 r-min centrifug\u00e1ltuk.-1<\/sup>\u00a010 percig h\u0171t\u00f6tt centrifug\u00e1ban. Ezut\u00e1n a sz\u00edv\u00f3sz\u0171r\u0151n kereszt\u00fcl kapjuk a fel\u00fcl\u00fasz\u00f3 folyad\u00e9k sz\u00fcks\u00e9ges naringin enzim enzim folyad\u00e9kot. H\u0171ts\u00fck le 4 \u2103-on a k\u00e9s\u0151bbi felhaszn\u00e1l\u00e1sig.<\/h4>\r\n\r\n\r\n\r\n
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3. A kitoz\u00e1n immobiliz\u00e1lt naringin\u00e1z enzim el\u0151\u00e1ll\u00edt\u00e1sa<\/strong><\/h2>\r\n\r\n\r\n\r\n
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Vegy\u00fcnk 3,0 g kitoz\u00e1nt, 100 ml 2% ecetsavas oldat t\u00e9rfogatfrakci\u00f3j\u00e1val feloldottuk, hogy 0,3 g - L-1<\/sup>\u00a0kitoz\u00e1n kolloid oldatot, 20 percig szonik\u00e1ztuk a l\u00e9gbubor\u00e9kok elt\u00e1vol\u00edt\u00e1sa \u00e9rdek\u00e9ben, \u00e9s hagytuk, hogy teljesen felold\u00f3djon. A kitoz\u00e1n kolloid oldatot cseppenk\u00e9nt fecskendezt\u00fck be egy t\u0171vel 0,5 g - L-1<\/sup>\u00a0NaOH-kondenz\u00e1tum (1000 ml), hogy sima pelletet k\u00e9pezzen. Hagyjuk \u00e1llni szobah\u0151m\u00e9rs\u00e9kleten 3 \u00f3r\u00e1n \u00e1t, majd a pelleteket t\u00f6bbsz\u00f6r mossuk desztill\u00e1lt v\u00edzzel semlegesre, \u00e9s a vizet sz\u0171r\u0151pap\u00edrral felsz\u00edvtuk, hogy megkapjuk a kitoz\u00e1n mikrog\u00f6mb\u00f6ket. 0,4 g kitoz\u00e1n mikrog\u00f6mb\u00f6t megm\u00e9rt\u00fcnk, 0,4 ml 6% t\u00e9rfogatr\u00e9sszel rendelkez\u0151 glutaraldehidet adtunk hozz\u00e1, \u00e9s a mikrog\u00f6mb\u00f6ket ism\u00e9telten desztill\u00e1lt v\u00edzzel mostuk, am\u00edg \u00e1lland\u00f3 h\u0151m\u00e9rs\u00e9klet \u00e9s 25 \u2103 150 r-min rezg\u00e9s ut\u00e1n nem volt glutaraldehid a mikrog\u00f6mb\u00f6k fel\u00fclet\u00e9n.-1<\/sup>\u00a0A t\u00e9rh\u00e1l\u00f3s\u00edtott kitoz\u00e1n mikrog\u00f6mb\u00f6ket sz\u0171r\u0151pap\u00edrral t\u00f6rt\u00e9n\u0151 sz\u00e1r\u00edt\u00e1ssal nyert\u00fck.<\/h4>\r\n\r\n\r\n\r\n
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\u00a0Azt mondta, 0,4 g kitoz\u00e1n mikrog\u00f6mb\u00f6k, adjunk hozz\u00e1 0,4 mL tag sz\u00e1ma hitelek 6% glutaraldehid, 150 r - min-1<\/sup>\u00a025 \u2103 h\u0151m\u00e9rs\u00e9kleten oszcill\u00e1ci\u00f3 2 \u00f3ra eltelt\u00e9vel t\u00f6bbsz\u00f6r mosott desztill\u00e1lt v\u00edzzel mikro- g\u00f6mb fel\u00fclet\u00e9re nem pentil-dialdehid \u00e9s sz\u0171r\u0151pap\u00edr sz\u00e1r\u00edtjuk, hogy keresztk\u00f6t\u00e9s\u0171 kitoz\u00e1n mikrog\u00f6mb\u00f6k. Vegy\u00fcnk 4 ml naringin\u00e1z enzimoldatot, adjuk hozz\u00e1 0,4 g t\u00e9rh\u00e1l\u00f3s\u00edtott kitoz\u00e1n mikrog\u00f6mbh\u00f6z, r\u00e1zzuk \u00e1lland\u00f3 25 \u2103 h\u0151m\u00e9rs\u00e9kleten, 150 r-min-1<\/sup>\u00a02 \u00f3r\u00e1n kereszt\u00fcl, majd tegye h\u0171t\u0151szekr\u00e9nybe 4 \u2103-on 4 \u00f3r\u00e1ra adszorpci\u00f3s csatol\u00e1sra. \u00d6bl\u00edtse le a hordoz\u00f3 fel\u00fclet\u00e9n visszamaradt enzimoldatot pH 4,5 pufferrel, sz\u00edvja fel a vizet a sz\u0171r\u0151pap\u00edrra, hogy immobiliz\u00e1lt naringin\u00e1zt kapjon, \u00e9s t\u00e1rolja h\u0171t\u0151szekr\u00e9nyben 4 \u2103-on k\u00e9s\u0151bbi felhaszn\u00e1l\u00e1sra.<\/h4>\r\n\r\n\r\n\r\n
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4. M\u00e1gneses szil\u00edcium-alap\u00fa kitoz\u00e1n ( MSC) mikrog\u00f6mb\u00f6k el\u0151\u00e1ll\u00edt\u00e1sa<\/strong><\/h2>\r\n\r\n\r\n\r\n
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A m\u00e1gneses szil\u00edcium alap\u00fa nanor\u00e9szecsk\u00e9k ( MS) el\u0151\u00e1ll\u00edt\u00e1si folyamata az 1. \u00e1br\u00e1n l\u00e1that\u00f3.<\/h4>\r\n\r\n\r\n\r\n
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1. \u00e1bra M\u00e1gneses szil\u00edcium alap\u00fa nanor\u00e9szecsk\u00e9k el\u0151\u00e1ll\u00edt\u00e1s\u00e1nak v\u00e1zlata<\/h4>\r\n\r\n\r\n\r\n
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( 1) El\u0151sz\u00f6r is, a m\u00e1gneses Fe3<\/sub>O4<\/sub>\u00a0nanor\u00e9szecsk\u00e9ket citromsavval m\u00f3dos\u00edtott k\u00e9miai ko-precipit\u00e1ci\u00f3s m\u00f3dszerrel \u00e1ll\u00edtottuk el\u0151, \u00e9s a k\u00eds\u00e9rleti elj\u00e1r\u00e1s a k\u00f6vetkez\u0151: 2,25 g FeCl3<\/sub>-6H2<\/sub>O \u00e9s 1,61 g FeSO4<\/sub>-7H2<\/sub>O-t adunk 100 ml ionmentes\u00edtett v\u00edzzel t\u00f6lt\u00f6tt h\u00e1romnyak\u00fa lombikba, a rendszer h\u0151m\u00e9rs\u00e9klet\u00e9t nitrog\u00e9ng\u00e1zzal 85 \u2103-ra meleg\u00edtett\u00fck. Keverj\u00fck 1000 r-min alatt-1<\/sup>\u00a0\u00e9s gyorsan \u00f6nts\u00fcnk r\u00e1 10 ml t\u00f6m\u00e9ny amm\u00f3niavizet. Miut\u00e1n az oldat megfeketedik, adjunk hozz\u00e1 153 mg n\u00e1trium-citr\u00e1tot. A 30 perces reakci\u00f3 ut\u00e1n v\u00e1lasszuk sz\u00e9t a termel\u00e9st m\u00e1gnessel, \u00e9s mossuk a szil\u00e1rd f\u00e1zist t\u00f6bbsz\u00f6r deioniz\u00e1lt v\u00edzzel \u00e9s etanollal. A Fe3<\/sub>O4<\/sub>\u00a0a kapott nanor\u00e9szecsk\u00e9ket k\u00e9s\u0151bbi felhaszn\u00e1l\u00e1s c\u00e9lj\u00e1b\u00f3l megsz\u00e1r\u00edtj\u00e1k.<\/h4>\r\n\r\n\r\n\r\n
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( 2) Ezut\u00e1n szol-g\u00e9l m\u00f3dszerrel m\u00e1gneses szil\u00edcium alap\u00fa nanor\u00e9szecsk\u00e9ket (MS) \u00e1ll\u00edtottunk el\u0151, amelyeket az 1. \u00e1bra mutat. A k\u00eds\u00e9rleti l\u00e9p\u00e9sek a k\u00f6vetkez\u0151k: 0,1 g Fe3<\/sub>O4<\/sub>\u00a0nanor\u00e9szecsk\u00e9ket egyenletesen eloszlattuk egy kevert oldatban, amely 40 ml v\u00edzmentes etanolt, 10 ml desztill\u00e1lt vizet \u00e9s 1,2 ml t\u00f6m\u00e9ny amm\u00f3niavizet tartalmazott. 300 r-min kever\u00e9si k\u00f6r\u00fclm\u00e9nyek k\u00f6z\u00f6tt-1<\/sup>, Fe3<\/sub>O4<\/sub>\u00a0nanor\u00e9szecsk\u00e9ket adtunk a 30 ml v\u00edzmentes etanolban diszperg\u00e1lt TEOS-hoz (0,4 ml), \u00e9s a szil\u00edcium alap\u00fa nanor\u00e9szecsk\u00e9k reakci\u00f3j\u00e1t 12 \u00f3r\u00e1n kereszt\u00fcl v\u00e9gezt\u00fck szobah\u0151m\u00e9rs\u00e9kleten. A term\u00e9keket t\u00f6bbsz\u00f6r mostuk v\u00edzmentes etanollal \u00e9s desztill\u00e1lt v\u00edzzel, hogy m\u00e1gneses szil\u00edcium-dioxid alap\u00fa nanor\u00e9szecsk\u00e9ket (MS) kapjunk, amelyeket a k\u00e9s\u0151bbi felhaszn\u00e1l\u00e1s c\u00e9lj\u00e1b\u00f3l megsz\u00e1r\u00edtottunk.<\/h4>\r\n\r\n\r\n\r\n
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Az MSC mikrog\u00f6mb\u00f6ket emulzi\u00f3s t\u00e9rh\u00e1l\u00f3s\u00edt\u00e1si m\u00f3dszerrel \u00e1ll\u00edtottuk el\u0151, amint azt a 2. \u00e1bra mutatja. A k\u00eds\u00e9rleti l\u00e9p\u00e9sek a k\u00f6vetkez\u0151k voltak: Az 5,0% t\u00e9rfogatt\u00f6meg\u0171 ecetsavoldatban oldott kitoz\u00e1nnal k\u00e9sz\u00edtett 2,5% t\u00f6megt\u00f6meg\u0171 10 ml kitoz\u00e1noldathoz egyenletesen eloszlatva 0,125 g m\u00e1gneses szil\u00edcium-dioxid nanor\u00e9szecsk\u00e9ket (MS), majd a kitoz\u00e1noldatot cseppenk\u00e9nt hozz\u00e1adtuk egy 60 ml foly\u00e9kony paraffinviaszt \u00e9s 1,8 ml twain 80-at tartalmaz\u00f3 emulzi\u00f3hoz. Miut\u00e1n 30 percig kevertett\u00fck 40 \u2103-os v\u00edzf\u00fcrd\u0151 \u00e9s 800 r-min felt\u00e9telekkel-1<\/sup>\uff0c hozz\u00e1adunk 2,0 mL glutaraldehidet \u00e9s 1 \u00f3r\u00e1n \u00e1t reag\u00e1lunk, majd a rendszer pH-j\u00e1t 1 mol-L-1<\/sup>\u00a0NaOH-val, \u00e9s a reakci\u00f3t 1 \u00f3r\u00e1n \u00e1t folytattuk. A term\u00e9keket t\u00f6bbsz\u00f6r mostuk petr\u00f3leum\u00e9terrel, etanollal \u00e9s desztill\u00e1lt v\u00edzzel, hogy MSC mikrog\u00f6mb\u00f6ket kapjunk, amelyeket v\u00e1kuumban sz\u00e1r\u00edtottunk a k\u00e9s\u0151bbi felhaszn\u00e1l\u00e1s c\u00e9lj\u00e1b\u00f3l.<\/h4>\r\n\r\n\r\n\r\n
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2. \u00e1bra M\u00e1gneses szil\u00edcium alap\u00fa kitoz\u00e1n mikrog\u00f6mb\u00f6k el\u0151\u00e1ll\u00edt\u00e1s\u00e1nak v\u00e1zlata<\/h4>\r\n\r\n\r\n\r\n
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5. Epoxi alap\u00fa m\u00e1gneses szil\u00edcium alap\u00fa kitoz\u00e1n mikrog\u00f6mb\u00f6k el\u0151\u00e1ll\u00edt\u00e1sa ( MSCE)<\/strong><\/h2>\r\n\r\n\r\n\r\n
\"\"<\/figure>\r\n\r\n\r\n\r\n

0,5 g sz\u00e1r\u00edtott, a 4. m\u00f3dszerrel el\u0151\u00e1ll\u00edtott MSC mikrog\u00f6mb\u00f6t lem\u00e9rt\u00fcnk, \u00e9s egy 50 ml-es Erlenmeyer-lombikba adtuk. Ezut\u00e1n 2,0 ml NaOH-oldatot, epikrolohidrin-oldatot \u00e9s NaBH4<\/sub>\u00a0oldatot adtunk a lombikba egy bizonyos koncentr\u00e1ci\u00f3ban. A reakci\u00f3t pedig egy bizonyos ideig \u00e1lland\u00f3 h\u0151m\u00e9rs\u00e9klet-ingadoz\u00e1ssal v\u00e9gezt\u00fck. Az MSCE el\u0151\u00e1ll\u00edt\u00e1s\u00e1nak reakci\u00f3mechanizmusa a 3. \u00e1br\u00e1n l\u00e1that\u00f3.<\/h4>\r\n\r\n\r\n\r\n
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3. \u00e1bra Az epoxi alap\u00fa m\u00e1gneses szil\u00edcium alap\u00fa kitoz\u00e1n mikrog\u00f6mb\u00f6k el\u0151\u00e1ll\u00edt\u00e1s\u00e1nak elve<\/h4>\r\n\r\n\r\n\r\n
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Az aktiv\u00e1l\u00e1s ut\u00e1n az aktiv\u00e1lt mikrog\u00f6mb\u00f6ket t\u00f6bbsz\u00f6r desztill\u00e1lt v\u00edzzel mossuk, am\u00edg a fel\u00fcleten nem maradnak szabad OH- \u00e9s epoxicsoportok, hogy epoxi alap\u00fa m\u00e1gneses szil\u00edcium alap\u00fa kitoz\u00e1n mikrog\u00f6mb\u00f6ket ( MSCE) kapjunk. A kimutat\u00e1si m\u00f3dszer a k\u00f6vetkez\u0151: vegy\u00fcnk 3,0 ml mos\u00e1si fel\u00fcl\u00fasz\u00f3t, \u00e9s adjunk hozz\u00e1 1~2 csepp fenolftaleint. R\u00e1zogat\u00e1s ut\u00e1n, ha nem v\u00e1ltozott pirosra, akkor az azt jelenti, hogy nincs szabad OH- az MSCE-vel; vegy\u00fcnk 3,0 mL mos\u00e1si fel\u00fcl\u00fasz\u00f3t, \u00e9s adjunk hozz\u00e1 3,0 mL 1,3 mol-L-1<\/sup>\u00a0n\u00e1trium-tioszulf\u00e1t \u00e9s 1 ~ 2 csepp fenolftalein, ha az oldat nem v\u00e1lt v\u00f6r\u00f6sre r\u00e1z\u00e1s ut\u00e1n, ez azt jelenti, hogy nincsenek szabad epoxi csoportok MSCE-vel. Az epoxicsoport kimutat\u00e1s\u00e1nak reakci\u00f3egyenlete a k\u00f6vetkez\u0151:<\/h4>\r\n\r\n\r\n\r\n
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6 MSCE immobiliz\u00e1lt naringin\u00e1z enzim el\u0151\u00e1ll\u00edt\u00e1sa<\/strong><\/h4>\r\n\r\n\r\n\r\n
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5,0 g 5. m\u00f3dszerrel k\u00e9sz\u00edtett MSCE-t adtunk 50 ml naringin\u00e1z enzimoldathoz egy bizonyos pH-\u00e9rt\u00e9kkel, \u00e9s a reakci\u00f3t \u00e1lland\u00f3 h\u0151m\u00e9rs\u00e9klet\u0171 v\u00edzf\u00fcrd\u0151 alatt oszcill\u00e1ltuk, amint azt a 4. \u00e1bra mutatja. A reakci\u00f3t k\u00f6vet\u0151en a mikrog\u00f6mb\u00f6ket t\u00f6bbsz\u00f6r mostuk pufferoldattal, am\u00edg a mikrog\u00f6mb\u00f6k fel\u00fclet\u00e9n nem tal\u00e1ltunk szabad naringin\u00e1zt. A mikrog\u00f6mb\u00f6ket Brinella-t\u00f6lcs\u00e9rrel lecsapoltuk, hogy MSCE immobiliz\u00e1lt naringin\u00e1z enzimet kapjunk, amelyet 4 \u2103-on h\u0171t\u00f6tt\u00fcnk a k\u00e9s\u0151bbi felhaszn\u00e1l\u00e1sra.\u00a0<\/h4>\r\n\r\n\r\n\r\n
\"\"<\/figure>\r\n\r\n\r\n\r\n

4. \u00e1bra Az MSCE immobiliz\u00e1lt naringin\u00e1z enzim el\u0151\u00e1ll\u00edt\u00e1s\u00e1nak elve<\/h4>\r\n\r\n\r\n\r\n

L\u00e9pjen kapcsolatba vel\u00fcnk most!<\/b><\/strong><\/h2>\r\n

Ha sz\u00fcks\u00e9ge van Price-ra, k\u00e9rj\u00fck, t\u00f6ltse ki el\u00e9rhet\u0151s\u00e9g\u00e9t az al\u00e1bbi \u0171rlapon, \u00e1ltal\u00e1ban 24 \u00f3r\u00e1n bel\u00fcl felvessz\u00fck \u00d6nnel a kapcsolatot. \u00d6n is k\u00fcldhet nekem e-mailt\u00a0info@longchangchemical.com<\/a><\/span>\u00a0munkaid\u0151ben ( 8:30-18:00 UTC+8 H.-Szombat ) vagy haszn\u00e1lja a weboldal \u00e9l\u0151 chatj\u00e9t, hogy azonnali v\u00e1laszt kapjon.<\/b><\/strong><\/h4>\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n
\u00d6sszet\u00e9tel Gl\u00fckoamil\u00e1z<\/span><\/a><\/td>\r\n9032-08-0<\/td>\r\n<\/tr>\r\n
Pullulanase<\/span><\/a><\/td>\r\n9075-68-7<\/td>\r\n<\/tr>\r\n
Xilan\u00e1z<\/span><\/a><\/td>\r\n37278-89-0<\/td>\r\n<\/tr>\r\n
Cellul\u00e1z<\/span><\/a><\/td>\r\n9012-54-8<\/td>\r\n<\/tr>\r\n
Naringin\u00e1z<\/span><\/a><\/td>\r\n9068-31-9<\/td>\r\n<\/tr>\r\n
\u03b2-Amil\u00e1z<\/span><\/a><\/td>\r\n9000-91-3<\/td>\r\n<\/tr>\r\n
Gl\u00fck\u00f3z-oxid\u00e1z<\/span><\/a><\/td>\r\n9001-37-0<\/td>\r\n<\/tr>\r\n
alfa-amil\u00e1z<\/td>\r\n9000-90-2<\/td>\r\n<\/tr>\r\n
Pektin\u00e1z<\/span><\/a><\/td>\r\n9032-75-1<\/td>\r\n<\/tr>\r\n
Peroxid\u00e1z<\/td>\r\n9003-99-0<\/td>\r\n<\/tr>\r\n
Lip\u00e1z<\/span><\/a><\/td>\r\n9001-62-1<\/td>\r\n<\/tr>\r\n
Katal\u00e1z<\/span><\/a><\/td>\r\n9001-05-2<\/td>\r\n<\/tr>\r\n
TANNASE<\/span><\/a><\/td>\r\n9025-71-2<\/td>\r\n<\/tr>\r\n
Elaszt\u00e1z<\/span><\/a><\/td>\r\n39445-21-1<\/td>\r\n<\/tr>\r\n
Ure\u00e1z<\/span><\/a><\/td>\r\n9002-13-5<\/td>\r\n<\/tr>\r\n
DEXTRANASE<\/span><\/a><\/td>\r\n9025-70-1<\/td>\r\n<\/tr>\r\n
L-laktil-dehidrogen\u00e1z<\/span><\/a><\/td>\r\n9001-60-9<\/td>\r\n<\/tr>\r\n
Dehidrogen\u00e1z mal\u00e1t<\/span><\/a><\/td>\r\n9001-64-3<\/td>\r\n<\/tr>\r\n
Koleszterin-oxid\u00e1z<\/span><\/a><\/td>\r\n9028-76-6<\/td>\r\n<\/tr>\r\n<\/tbody>\r\n<\/table>","protected":false},"excerpt":{"rendered":"

1. A naringin\u00e1z enzimaktivit\u00e1s kimutat\u00e1si m\u00f3dszere 1-1 Spektrofotometri\u00e1s m\u00f3dszer A para-nitrofenol spektrofotometri\u00e1s m\u00f3dszer p-nitrofenol- \u03b1-ramnosidot ( pNP- \u03b1- Rha ) \u00e9s p-nitrofenol-\u03b2-D-gl\u00fckopiranozidot ( pNP- \u03b2-Glu ) haszn\u00e1l szubsztr\u00e1tk\u00e9nt az \u03b1-ramnosid\u00e1z \u00e9s \u03b2-gl\u00fckozid\u00e1z enzimaktivit\u00e1s\u00e1nak kimutat\u00e1s\u00e1ra. Li Ping a p-nitrofenol spektrofotom\u00e9teres m\u00f3dszert alkalmazta, a k\u00eds\u00e9rletben Aspergillus niger \u03b2-gl\u00fckozid\u00e1z hozz\u00e1ad\u00e1s\u00e1val a p-nitrofenol -\u03b2-gl\u00fckozid ( pNPG ) oldat\u00e1ba enzimatikus hidrol\u00edzisre, \u00e9s az abszorbancia \u00e9rt\u00e9k\u00e9t [...].<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[108],"tags":[],"class_list":["post-4557","post","type-post","status-publish","format-standard","hentry","category-enzyme-news"],"yoast_head":"\nExperimental parameters of\u00a0naringinase immobilized on magnetic silicon based materials - Longchang Chemical<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/longchangchemical.com\/hu\/immobilized-on-magnetic-silicon-based-materials\/\" \/>\n<meta property=\"og:locale\" content=\"hu_HU\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Experimental parameters of\u00a0naringinase immobilized on magnetic silicon based materials - Longchang Chemical\" \/>\n<meta property=\"og:description\" content=\"1.\u00a0Detection method of naringinase enzyme activity 1-1\u00a0Spectrophotometer method The para nitrophenol spectrophotometric method\u00a0uses p-nitrophenol-\u00a0\u03b1-rhamnoside (\u00a0pNP-\u00a0\u03b1-\u00a0Rha\u00a0) and p-nitrophenol-\u03b2-D-glucopyranoside (\u00a0pNP-\u00a0\u03b2-Glu) as substrate, for detecting the enzyme activity of\u00a0the \u03b1-rhamnosidase\u00a0and \u03b2-glucosidase.\u00a0Li Ping\u00a0used the p-nitrophenol spectrophotometer method, in the experiment of\u00a0adding\u00a0Aspergillus niger \u03b2-glucosidase into the solution of\u00a0p-nitrophenol\u00a0-\u03b2-glucoside (\u00a0pNPG\u00a0) for enzymatic hydrolysis, and the value of absorbance was measured at […]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/longchangchemical.com\/hu\/immobilized-on-magnetic-silicon-based-materials\/\" \/>\n<meta property=\"og:site_name\" content=\"Longchang Chemical\" \/>\n<meta property=\"article:published_time\" content=\"2021-04-29T10:10:14+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2024-09-28T11:56:32+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/longchangchemical.com\/wp-content\/uploads\/2021\/04\/image-45.png\" \/>\n<meta name=\"author\" content=\"Longchang Chemical\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\/\/longchangchemical.com\/immobilized-on-magnetic-silicon-based-materials\/#article\",\"isPartOf\":{\"@id\":\"https:\/\/longchangchemical.com\/immobilized-on-magnetic-silicon-based-materials\/\"},\"author\":{\"name\":\"Longchang Chemical\",\"@id\":\"https:\/\/longchangchemical.com\/#\/schema\/person\/ecb1ad846136c6f1ca534b62978c6a8e\"},\"headline\":\"Experimental parameters of\u00a0naringinase immobilized on magnetic silicon based materials\",\"datePublished\":\"2021-04-29T10:10:14+00:00\",\"dateModified\":\"2024-09-28T11:56:32+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\/\/longchangchemical.com\/immobilized-on-magnetic-silicon-based-materials\/\"},\"wordCount\":1840,\"commentCount\":0,\"publisher\":{\"@id\":\"https:\/\/longchangchemical.com\/#organization\"},\"image\":{\"@id\":\"https:\/\/longchangchemical.com\/immobilized-on-magnetic-silicon-based-materials\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/longchangchemical.com\/wp-content\/uploads\/2021\/04\/image-45.png\",\"articleSection\":[\"Enzyme News\"],\"inLanguage\":\"hu\",\"potentialAction\":[{\"@type\":\"CommentAction\",\"name\":\"Comment\",\"target\":[\"https:\/\/longchangchemical.com\/immobilized-on-magnetic-silicon-based-materials\/#respond\"]}]},{\"@type\":\"WebPage\",\"@id\":\"https:\/\/longchangchemical.com\/immobilized-on-magnetic-silicon-based-materials\/\",\"url\":\"https:\/\/longchangchemical.com\/immobilized-on-magnetic-silicon-based-materials\/\",\"name\":\"Experimental parameters of\u00a0naringinase immobilized on magnetic silicon based materials - 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