{"id":4514,"date":"2021-04-22T07:03:58","date_gmt":"2021-04-22T07:03:58","guid":{"rendered":"https:\/\/longchangchemical.com\/?p=4514"},"modified":"2024-09-28T11:56:15","modified_gmt":"2024-09-28T11:56:15","slug":"overview-of-the-methods","status":"publish","type":"post","link":"https:\/\/longchangchemical.com\/hu\/overview-of-the-methods\/","title":{"rendered":"Az enzimek m\u00e1gneses biopolimer mikrog\u00f6mb\u00f6k\u00f6n t\u00f6rt\u00e9n\u0151 immobiliz\u00e1l\u00e1s\u00e1nak m\u00f3dszereinek \u00e1ttekint\u00e9se"},"content":{"rendered":"

A m\u00e1gneses biopolimer mikrog\u00f6mb\u00f6k olyan \u00faj kompozit anyagok, amelyek biol\u00f3giai anyagokat \u00e9s szervetlen m\u00e1gneses anyagokat egyes\u00edtenek, hogy m\u00e1gnesesen \u00e9rz\u00e9keny \u00e9s biol\u00f3giailag akt\u00edv mikrog\u00f6mb\u00f6ket alkossanak. Tulajdons\u00e1gai a szervetlen m\u00e1gneses anyagokt\u00f3l, a biol\u00f3giai anyagokt\u00f3l \u00e9s k\u00f6lcs\u00f6nhat\u00e1saik m\u00f3dszer\u00e9t\u0151l f\u00fcggnek. Jelenleg a legsz\u00e9lesebb k\u00f6rben haszn\u00e1lt \u00e9s vizsg\u00e1lt m\u00e1gneses anyag a Fe3<\/sub>O4<\/sub>\u00a0m\u00e1gneses nanor\u00e9szecsk\u00e9k. Nagy fajlagos fel\u00fclet\u00e9nek, j\u00f3 biokompatibilit\u00e1s\u00e1nak \u00e9s magas m\u00e1gneses v\u00e1lasz\u00e1nak k\u00f6sz\u00f6nhet\u0151en gyors elv\u00e1laszt\u00e1st \u00e9s c\u00e9lzott mozg\u00e1st tud el\u00e9rni, \u00edgy sz\u00e9les k\u00f6rben alkalmazhat\u00f3 az \u00e9lelmiszer- \u00e9s orvosi ell\u00e1t\u00e1sban, a k\u00f6rnyezetv\u00e9delemben \u00e9s m\u00e1s ter\u00fcleteken. A biopolimer anyagok k\u00f6z\u00e9 tartozik els\u0151sorban a kitoz\u00e1n, a n\u00e1trium-algin\u00e1t, a zselatin stb. K\u00f6z\u00fcl\u00fck a kitoz\u00e1n a legink\u00e1bb vizsg\u00e1lt biol\u00f3giai anyag a j\u00f3 biokompatibilit\u00e1s, a meg\u00fajul\u00f3 er\u0151forr\u00e1sok \u00e9s a biol\u00f3giai lebonthat\u00f3s\u00e1g saj\u00e1t jellemz\u0151i miatt. A kitoz\u00e1nb\u00f3l \u00e9s m\u00e1gneses anyagb\u00f3l \u00e1ll\u00f3 \u00faj t\u00edpus\u00fa kompozit anyag mindkett\u0151 kiv\u00e1l\u00f3 tulajdons\u00e1gokkal rendelkezik, \u00edgy sz\u00e9lesk\u00f6r\u0171 alkalmaz\u00e1si lehet\u0151s\u00e9gekkel rendelkezik k\u00fcl\u00f6nb\u00f6z\u0151 ter\u00fcleteken.<\/h4>\r\n\r\n\r\n\r\n
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1. M\u00e1gneses biopolimer mikrog\u00f6mb\u00f6k szerkezete \u00e9s tulajdons\u00e1gai<\/strong><\/h2>\r\n\r\n\r\n\r\n
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A m\u00e1gneses biopolimer mikrog\u00f6mb\u00f6k szerkezete h\u00e1romf\u00e9le lehet: (1) mag-h\u00e9j szerkezet; (2) hibrid szerkezet; (3) t\u00f6bbr\u00e9teg\u0171 szendvicsszerkezet, amint azt az 1. \u00e1bra mutatja.<\/h4>\r\n\r\n\r\n\r\n
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1. \u00e1bra A m\u00e1gneses biopolimer mikrog\u00f6mb\u00f6k szerkezete<\/h4>\r\n\r\n\r\n\r\n
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A m\u00e1gneses biopolimer mikrog\u00f6mb\u00f6k sz\u00e1mos kiemelked\u0151 tulajdons\u00e1ggal rendelkeznek, ami alkalmass\u00e1 teszi \u0151ket az enzimek immobiliz\u00e1l\u00e1s\u00e1ra. Ilyen p\u00e9ld\u00e1ul (1) a fel\u00fcleti hat\u00e1s. \u00c1ltal\u00e1ban, amikor a m\u00e1gneses biopolimer mikrog\u00f6mb\u00f6k r\u00e9szecskem\u00e9rete el\u00e9ri a mikron vagy ak\u00e1r a nanom\u00e9teres szintet, a fajlagos fel\u00fclet n\u00f6veked\u00e9s\u00e9vel a mikrog\u00f6mb\u00f6k csoports\u0171r\u0171s\u00e9ge \u00e9s szelekt\u00edv adszorpci\u00f3s teljes\u00edtm\u00e9nye is n\u0151, \u00e9s a mikrog\u00f6mb\u00f6k stabilit\u00e1sa jelent\u0151sen megn\u0151. (2) M\u00e1gneses hat\u00e1s. Amikor a Fe3<\/sub>O4<\/sub>\u00a0krist\u00e1ly kisebb, mint 30 nm, szuperparam\u00e1gnesess\u00e9ggel rendelkezik, azaz k\u00fcls\u0151 m\u00e1gneses t\u00e9r hat\u00e1s\u00e1ra nagyon nagy a m\u00e1gnesess\u00e9g. Amikor a k\u00fcls\u0151 m\u00e1gneses mez\u0151t elt\u00e1vol\u00edtj\u00e1k, m\u00e1gnesess\u00e9ge gyorsan elt\u0171nik, \u00edgy a mikrog\u00f6mb k\u00fcls\u0151 m\u00e1gneses t\u00e9r \u00e1llapot\u00e1ban m\u00e1gneses orient\u00e1ci\u00f3val rendelkezik, \u00e9s k\u00e9pes gyorsan elv\u00e1lni a nem m\u00e1gneses anyagokt\u00f3l, \u00e9s m\u00e1gneses t\u00e9rben nem m\u00e1gnesez\u0151dik tart\u00f3san, \u00edgy nem befoly\u00e1solja a k\u00e9s\u0151bbi felhaszn\u00e1l\u00e1st. (3) Biokompatibilit\u00e1s. A term\u00e9szetben a biol\u00f3giai anyagok, p\u00e9ld\u00e1ul a feh\u00e9rj\u00e9k \u00e9s a poliszacharidok biokompatibilit\u00e1ssal rendelkeznek, ami miatt fontos alkalmaz\u00e1sokkal rendelkeznek az orvosbiol\u00f3giai technik\u00e1ban. (4) Funkcion\u00e1lis alapjellemz\u0151k. Az olyan biol\u00f3giai anyagok, mint a kitoz\u00e1n \u00e9s a n\u00e1trium-algin\u00e1t, b\u0151s\u00e9gesen rendelkeznek akt\u00edv csoportokkal (-OH, -COOH, -NH2), amelyek kovalensen \u00f6sszekapcsolhat\u00f3k biol\u00f3giailag akt\u00edv anyagokkal, vagy bizonyos k\u00e9miai csoportokkal m\u00f3dos\u00edthat\u00f3k.<\/h4>\r\n\r\n\r\n\r\n
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2. M\u00e1gneses biopolimer mikrog\u00f6mb\u00f6k el\u0151\u00e1ll\u00edt\u00e1sa<\/strong><\/h2>\r\n\r\n\r\n\r\n
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A m\u00e1gneses biopolimer mikrog\u00f6mb\u00f6k el\u0151\u00e1ll\u00edt\u00e1sa k\u00e9t l\u00e9p\u00e9sre oszlik. Az els\u0151 l\u00e9p\u00e9s a m\u00e1gneses nanor\u00e9szecsk\u00e9k el\u0151\u00e1ll\u00edt\u00e1sa. A jelenleg haszn\u00e1lt m\u00f3dszerek a Fe3<\/sub>O4<\/sub>\u00a0A m\u00e1gneses nanor\u00e9szecsk\u00e9k el\u0151\u00e1ll\u00edt\u00e1sa els\u0151sorban a k\u00e9miai ko-lev\u00e1laszt\u00e1s, a vas-s\u00f3 termikus boml\u00e1sa, a mikroemulzi\u00f3 \u00e9s a hidrotermikus m\u00f3dszerek k\u00f6z\u00e9 tartozik. Ezek k\u00f6z\u00fcl a k\u00e9miai ko-precipit\u00e1ci\u00f3s m\u00f3dszer egyszer\u0171 \u00e9s k\u00e9nyelmesen m\u0171k\u00f6dtethet\u0151, \u00e9s ez a leggyakrabban alkalmazott el\u0151\u00e1ll\u00edt\u00e1si m\u00f3dszer. A k\u00e9miai ko-lev\u00e1laszt\u00e1si m\u00f3dszer elve a Fe3<\/sub>O4<\/sub>\u00a0a vas-oxid szintetiz\u00e1l\u00e1sa egy bizonyos ar\u00e1ny\u00fa Fe2+<\/sup>\u00a0\u00e9s Fe3+<\/sup>\u00a0(1:2) anaerob k\u00f6r\u00fclm\u00e9nyek k\u00f6z\u00f6tt, majd gyorsan l\u00fagot (amm\u00f3nia vagy NaOH) adunk hozz\u00e1. Xu \u00e9s munkat\u00e1rsai a k\u00e9miai ko-precipit\u00e1ci\u00f3s m\u00f3dszert alkalmazt\u00e1k 4,34 mmol FeCl2\u9458<\/sub>4H2O \u00e9s 8,67 mmol FeCl3<\/sub>-6H2<\/sub>O, \u00e9s a rendszert 85 \u2103-ra meleg\u00edtj\u00fck nitrog\u00e9n alatt. Miut\u00e1n teljesen felold\u00f3dott, gyorsan adjunk hozz\u00e1 25 ml t\u00f6m\u00e9ny amm\u00f3ni\u00e1t \u00e9s adjunk hozz\u00e1 bizonyos mennyis\u00e9g\u0171 n\u00e1trium-citr\u00e1tot, majd a Fe3<\/sub>O4<\/sub>\u00a0m\u00e1gneses nanor\u00e9szecsk\u00e9ket szintetiz\u00e1lnak j\u00f3 monodiszperzit\u00e1ssal \u00e9s m\u00e1gneses \u00e9rz\u00e9kenys\u00e9ggel.<\/h4>\r\n\r\n\r\n\r\n
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A m\u00e1gneses nanor\u00e9szecsk\u00e9k el\u0151\u00e1ll\u00edt\u00e1sa ut\u00e1n a m\u00e1gneses kitoz\u00e1n mikrog\u00f6mb\u00f6k el\u0151\u00e1ll\u00edt\u00e1sa \u00e9rdek\u00e9ben keresztk\u00f6t\u00e9st kell v\u00e9gezni kitoz\u00e1nnal. Jelenleg a m\u00e1gneses kitoz\u00e1n mikrog\u00f6mb\u00f6k szint\u00e9zis\u00e9nek m\u00f3dszerei f\u0151k\u00e9nt az emulzi\u00f3s t\u00e9rh\u00e1l\u00f3s\u00edt\u00e1si m\u00f3dszer, a permetsz\u00e1r\u00edt\u00e1si m\u00f3dszer, a fotok\u00e9miai m\u00f3dszer \u00e9s az in-situ m\u00f3dszer. Ezek k\u00f6z\u00fcl az emulzi\u00f3s t\u00e9rh\u00e1l\u00f3s\u00edt\u00f3 m\u00f3dszer az egyszer\u0171bb \u00e9s a legsz\u00e9lesebb k\u00f6rben haszn\u00e1lt. Az emulzi\u00f3s t\u00e9rh\u00e1l\u00f3s\u00edt\u00f3 m\u00f3dszer a Fe3<\/sub>O4<\/sub>\u00a0vagy m\u00e1gneses folyad\u00e9kot egy kevert folyad\u00e9kban, amely kitoz\u00e1nt, fel\u00fcletakt\u00edv anyagot \u00e9s olajf\u00e1zist tartalmaz, hogy v\u00edz az olajban mikroemulzi\u00f3s rendszert k\u00e9pezzen, majd adjunk hozz\u00e1 glutaraldehidet, a rendszerben a glutaraldehid \u00e9s a kitoz\u00e1n k\u00f6z\u00f6tt keresztk\u00f6t\u00e9si reakci\u00f3 megy v\u00e9gbe, hogy egy Schiff-b\u00e1zist hozzon l\u00e9tre, \u00e9s a kitoz\u00e1n h\u00e1l\u00f3zatba kapcsol\u00f3dik, majd bevonja a Feats-eket.3<\/sub>O4<\/sub>\u00a0(amint az a 2. \u00e1br\u00e1n l\u00e1that\u00f3). Jiang \u00e9s munkat\u00e1rsai emulzi\u00f3s t\u00e9rh\u00e1l\u00f3s\u00edt\u00e1si m\u00f3dszert alkalmaztak, Span 80, foly\u00e9kony paraffin \u00e9s glutaraldehid mint fel\u00fcletakt\u00edv anyag, diszperg\u00e1l\u00f3szer \u00e9s t\u00e9rh\u00e1l\u00f3s\u00edt\u00f3szer felhaszn\u00e1l\u00e1s\u00e1val, szab\u00e1lyos g\u00f6mb alak\u00fa \u00e9s sima fel\u00fclet\u0171 m\u00e1gneses kitoz\u00e1n mikrog\u00f6mb\u00f6k szint\u00e9zis\u00e9re.<\/h4>\r\n\r\n\r\n\r\n
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2. \u00e1bra M\u00e1gneses kitoz\u00e1n mikrog\u00f6mb\u00f6k szint\u00e9zis\u00e9nek v\u00e1zlata emulzi\u00f3s t\u00e9rh\u00e1l\u00f3s\u00edt\u00f3 m\u00f3dszerrel<\/h4>\r\n\r\n\r\n\r\n
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3. M\u00e1gneses kompozit mikrog\u00f6mb\u00f6k\u00f6n immobiliz\u00e1lt enzim<\/strong><\/h2>\r\n\r\n\r\n\r\n
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A m\u00e1gneses kitoz\u00e1n mikrog\u00f6mb\u00f6k sikeres el\u0151\u00e1ll\u00edt\u00e1sa ut\u00e1n az enzim felhaszn\u00e1l\u00e1s c\u00e9lj\u00e1b\u00f3l immobiliz\u00e1lhat\u00f3 a mikrog\u00f6mb\u00f6k\u00f6n. Mivel a kitoz\u00e1n akt\u00edv aminocsoportokban \u00e9s hidroxilcsoportokban gazdag, k\u00e9pes reag\u00e1lni karboxilcsoportokkal, aminocsoportokkal, epoxicsoportokkal, bifunkci\u00f3s csoportokkal stb. A m\u00e1gneses kitoz\u00e1n mikrog\u00f6mb\u00f6k csoportm\u00f3dos\u00edt\u00e1ssal vannak ell\u00e1tva, hogy megfeleljenek a k\u00fcl\u00f6nb\u00f6z\u0151 immobiliz\u00e1ci\u00f3s ig\u00e9nyeknek. A specifikus immobiliz\u00e1lt enzimek el\u0151\u00e1ll\u00edt\u00e1si m\u00f3dszereit az al\u00e1bbiakban funkcion\u00e1lis csoportok szerint mutatjuk be.<\/h4>\r\n\r\n\r\n\r\n
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1) Karboxil m\u00f3dos\u00edtott m\u00e1gneses kompozit mikrog\u00f6mb\u00f6k\u00f6n immobiliz\u00e1lt enzim<\/h3>\r\n\r\n\r\n\r\n

A karboxil-m\u00f3dos\u00edtott m\u00e1gneses kompozit mikrog\u00f6mb\u00f6k kovalensen k\u00f6t\u0151dhetnek az enzim aminocsoportj\u00e1hoz, miut\u00e1n vizes oldatban karbodiimid-kapcsol\u00e1ssal aktiv\u00e1lt\u00e1k \u0151ket, ez\u00e1ltal immobiliz\u00e1lva az enzimmolekul\u00e1t a m\u00e1gneses kompozit mikrog\u00f6mb\u00f6k\u00f6n (3. \u00e1bra). Zhu Yihua \u00e9s m\u00e1sok egy tov\u00e1bbfejlesztett szuszpenzi\u00f3s polimeriz\u00e1ci\u00f3s m\u00f3dszert alkalmaztak a sztirolnal kezelt m\u00e1gneses folyad\u00e9k \u00e9s a metil-akril\u00e1t monomer kopolimeriz\u00e1ci\u00f3j\u00e1ra a t\u00e9rh\u00e1l\u00f3s\u00edt\u00f3 monomer divinilbenzol seg\u00edts\u00e9g\u00e9vel, majd alk\u00e1li hidrol\u00edzist alkalmazva j\u00f3 monodiszperzit\u00e1s\u00fa \u00e9s gazdag karboxilcsoportokkal rendelkez\u0151 m\u00e1gneses kompozitot kaptak. A mikrog\u00f6mb\u00f6ket a lakt\u00e1z immobiliz\u00e1l\u00e1s\u00e1ra haszn\u00e1lj\u00e1k, miut\u00e1n karbodiimid-kapcsol\u00e1ssal aktiv\u00e1lt\u00e1k. A legnagyobb aktivit\u00e1s k\u00f6r\u00fclbel\u00fcl 360 U-g-1<\/sup>, \u00e9s az enzim keresztk\u00f6t\u00e9si hat\u00e9konys\u00e1ga k\u00f6r\u00fclbel\u00fcl 20%.<\/h4>\r\n\r\n\r\n\r\n
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3. \u00e1bra A karboxil m\u00f3dos\u00edtott m\u00e1gneses kompozit mikrog\u00f6mb\u00f6k\u00f6n immobiliz\u00e1lt enzim el\u0151\u00e1ll\u00edt\u00e1s\u00e1nak sematikus \u00e1br\u00e1ja<\/h4>\r\n\r\n\r\n\r\n
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2) Amin\u00f3val m\u00f3dos\u00edtott m\u00e1gneses kompozit mikrog\u00f6mb\u00f6k immobiliz\u00e1lt enzimmel<\/h3>\r\n\r\n\r\n\r\n

Miut\u00e1n az amino-m\u00f3dos\u00edtott m\u00e1gneses kompozit mikrog\u00f6mb\u00f6ket megfelel\u0151 mennyis\u00e9g\u0171 glutaraldehiddel \u00f6sszekapcsolt\u00e1k \u00e9s aktiv\u00e1lt\u00e1k, azok kovalensen k\u00f6t\u0151dhetnek az enzim aminocsoportj\u00e1hoz, ez\u00e1ltal immobiliz\u00e1lva az enzimmolekul\u00e1kat a m\u00e1gneses mikrog\u00f6mb\u00f6k\u00f6n (4. \u00e1bra). Liu Yu \u00e9s munkat\u00e1rsai egym\u00e1s ut\u00e1n \u00e1ll\u00edtottak el\u0151 monodiszperz m\u00e1gneses SiO2<\/sub>\u00a0r\u00e9szecsk\u00e9ket k\u00e9miai ko-precipit\u00e1ci\u00f3s m\u00f3dszerrel \u00e9s szol-g\u00e9l m\u00f3dszerrel, aminocsoportokkal m\u00f3dos\u00edtottuk \u0151ket szil\u00e1n-kapcsol\u00f3szerrel, \u00e9s a lacc\u00e1zt glutaraldehiddel mint t\u00e9rh\u00e1l\u00f3s\u00edt\u00f3szerrel immobiliz\u00e1ltuk. Az eredm\u00e9nyek azt mutatt\u00e1k, hogy az immobiliz\u00e1lt lacc\u00e1z 4 \u00f3r\u00e1n kereszt\u00fcl 60 \u2103 \u00e1lland\u00f3 h\u0151m\u00e9rs\u00e9kleten tartott\u00e1k, \u00e9s m\u00e9g mindig 60,9% enzimaktivit\u00e1ssal rendelkezett, \u00e9s 10 ciklusos haszn\u00e1lat ut\u00e1n m\u00e9g mindig t\u00f6bb mint 55% enzimaktivit\u00e1ssal rendelkezett, \u00e9s h\u0151stabilit\u00e1sa \u00e9s m\u0171k\u00f6d\u00e9si stabilit\u00e1sa nyilv\u00e1nval\u00f3an javult.<\/p>\r\n\r\n\r\n\r\n

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4. \u00e1bra Az amino-m\u00f3dos\u00edtott m\u00e1gneses kompozit mikrog\u00f6mb\u00f6k\u00f6n immobiliz\u00e1lt enzim el\u0151\u00e1ll\u00edt\u00e1s\u00e1nak sematikus \u00e1br\u00e1ja<\/p>\r\n\r\n\r\n\r\n

3) Epoxi m\u00f3dos\u00edtott m\u00e1gneses kompozit mikrog\u00f6mb\u00f6k immobiliz\u00e1lt enzimmel<\/h3>\r\n\r\n\r\n\r\n

Az epoxi csoport egy rendk\u00edv\u00fcl akt\u00edv csoport. K\u00f6zvetlen\u00fcl, m\u00f3dos\u00edt\u00e1s n\u00e9lk\u00fcl kovalens m\u00f3don biol\u00f3giai csoportokhoz k\u00f6thet\u0151. Ez\u00e9rt miut\u00e1n az epoxival m\u00f3dos\u00edtott m\u00e1gneses kompozit mikrog\u00f6mb\u00f6k kovalensen k\u00f6t\u0151dnek az enzim aminocsoportj\u00e1hoz az enzim megk\u00f6t\u00e9se \u00e9rdek\u00e9ben, a molekul\u00e1k immobiliz\u00e1l\u00f3dnak a m\u00e1gneses mikrog\u00f6mb\u00f6k\u00f6n (5. \u00e1bra). Yong \u00e9s munkat\u00e1rsai olajsavval bevont m\u00e1gneses mikrog\u00f6mb\u00f6ket \u00e1ll\u00edtottak el\u0151 szuszpenzi\u00f3s polimeriz\u00e1ci\u00f3val. A metanolos aktiv\u00e1l\u00e1s ut\u00e1n kapott hidrofil epoxi alap\u00fa m\u00e1gneses mikrog\u00f6mb\u00f6ket haszn\u00e1lt\u00e1k a lip\u00e1z immobiliz\u00e1l\u00e1s\u00e1ra. Az immobiliz\u00e1lt enzimaktivit\u00e1s visszatart\u00e1si ar\u00e1nya 64,2%, \u00e9s a stabilit\u00e1sa jelent\u0151sen javult.<\/h4>\r\n\r\n\r\n\r\n
\"\"<\/figure>\r\n\r\n\r\n\r\n

5. \u00e1bra Az epoxi alap\u00fa m\u00e1gneses kompozit mikrog\u00f6mb\u00f6k\u00f6n immobiliz\u00e1lt enzim el\u0151\u00e1ll\u00edt\u00e1s\u00e1nak sematikus \u00e1br\u00e1ja<\/p>\r\n\r\n\r\n\r\n

\"\"<\/figure>\r\n\r\n\r\n\r\n

4) Bifunkci\u00f3s csoportokkal m\u00f3dos\u00edtott m\u00e1gneses kompozit mikrog\u00f6mb\u00f6k\u00f6n immobiliz\u00e1lt enzimek<\/h3>\r\n\r\n\r\n\r\n
\"\"<\/figure>\r\n\r\n\r\n\r\n

A bifunkcion\u00e1lis m\u00e1gneses mikrog\u00f6mb\u00f6k\u00f6n el\u0151sz\u00f6r az enzimmolekula karboxilcsoportjai \u00e9s a mikrog\u00f6mb\u00f6k aminocsoportjai ionk\u00f6lcs\u00f6nhat\u00e1s r\u00e9v\u00e9n gyorsan immobiliz\u00e1l\u00f3dnak a hordoz\u00f3n, \u00e9s az ionk\u00f6lcs\u00f6nhat\u00e1s r\u00e9v\u00e9n immobiliz\u00e1lt enzim szulfhidril- \u00e9s aminocsoportjain kereszt\u00fcl kovalens k\u00f6lcs\u00f6nhat\u00e1sba l\u00e9p a hordoz\u00f3n l\u00e9v\u0151 epoxicsoportokkal. Tegye azt tov\u00e1bb fix\u00e1ltt\u00e1 (amint az a 6. \u00e1br\u00e1n l\u00e1that\u00f3), amelynek kett\u0151s jellemz\u0151je van: gyors fix\u00e1l\u00e1s ionhat\u00e1s r\u00e9v\u00e9n \u00e9s szil\u00e1rd fix\u00e1l\u00e1s kovalens k\u00f6t\u00e9s r\u00e9v\u00e9n. Li Xiutao \u00e9s munkat\u00e1rsai h\u00e1rom v\u00e9letlenszer\u0171 kopolimer ecsetet vezettek be a divinil-benzollal t\u00e9rh\u00e1l\u00f3s\u00edtott poliakrilsav mikrog\u00f6mb\u00f6k fel\u00fclet\u00e9re Fe3<\/sub>O4<\/sub>\u00a0nanor\u00e9szecsk\u00e9kkel diszperg\u00e1lt\u00e1k, majd a penicillin G acil\u00e1z immobiliz\u00e1l\u00e1s\u00e1ra haszn\u00e1lt\u00e1k. Az eredm\u00e9nyek azt mutatj\u00e1k, hogy az egyszerre epoxi csoportot \u00e9s aminocsoportot is tartalmaz\u00f3 immobiliz\u00e1lt enzim aktivit\u00e1sa \u00e9s az enzimaktivit\u00e1s visszanyer\u00e9si ar\u00e1nya a legmagasabb, immobiliz\u00e1ci\u00f3s kinetik\u00e1ja jobb, mint a csak epoxit tartalmaz\u00f3 m\u00e1gneses mikrog\u00f6mb\u00f6k\u00e9, \u00e9s optim\u00e1lis pH-\u00e9rt\u00e9ke \u00e9s h\u0151m\u00e9rs\u00e9kleti stabilit\u00e1sa magasabb, mint a szabad enzim\u00e9, \u00e9s az enzimaktivit\u00e1s megtartja a 70%-t 10 alkalommal t\u00f6rt\u00e9n\u0151 ism\u00e9telt haszn\u00e1lat ut\u00e1n.<\/h4>\r\n\r\n\r\n\r\n
\"\"<\/figure>\r\n\r\n\r\n\r\n

6. \u00e1bra A bifunkci\u00f3s csoportokkal m\u00f3dos\u00edtott m\u00e1gneses kompozit mikrog\u00f6mb\u00f6k\u00f6n immobiliz\u00e1lt enzim el\u0151\u00e1ll\u00edt\u00e1s\u00e1nak sematikus \u00e1br\u00e1ja<\/h4>\r\n\r\n\r\n\r\n
\"\"<\/figure>\r\n\r\n\r\n\r\n

Az elm\u00falt \u00e9vekben, b\u00e1r a tud\u00f3sok k\u00fcl\u00f6nb\u00f6z\u0151 anyagokat haszn\u00e1ltak a naringin\u00e1z immobiliz\u00e1l\u00e1s\u00e1ra, p\u00e9ld\u00e1ul term\u00e9szetes biopolimer anyagokat, mint p\u00e9ld\u00e1ul kitoz\u00e1n, n\u00e1trium-algin\u00e1t \u00e9s selyemfeh\u00e9rje, szerves vegy\u00fcletek, mint p\u00e9ld\u00e1ul epoxigyanta \u00e9s polivinil-alkohol, akt\u00edv sz\u00e9n \u00e9s grafit-oxid sz\u00e9n anyagok, mint p\u00e9ld\u00e1ul ene, \u00e9s bizonyos kutat\u00e1si eredm\u00e9nyeket kaptak, de a t\u00e9nyleges debittering alkalmaz\u00e1sokban vannak probl\u00e9m\u00e1k, mint p\u00e9ld\u00e1ul az immobiliz\u00e1lt enzimek gyenge sav\u00e1ll\u00f3s\u00e1ga, lass\u00fa elv\u00e1laszt\u00e1s a gy\u00fcm\u00f6lcsl\u00e9t\u0151l vagy hi\u00e1nyos elv\u00e1laszt\u00e1s stb. A fenti probl\u00e9m\u00e1kkal kapcsolatban a k\u00f6vetkez\u0151 cikk r\u00e9szletesen bemutat egy kutat\u00e1si munk\u00e1t. Ebben a wokban a kutat\u00f3k egy olyan kompozit anyagot haszn\u00e1ltak, amelyet kitoz\u00e1n, m\u00e1gneses Fe3<\/sub>O4<\/sub>\u00a0nanor\u00e9szecsk\u00e9ket \u00e9s szil\u00edcium-dioxidot, \u00e9s a kompozit anyagot epoxi csoportokkal m\u00f3dos\u00edtott\u00e1k, majd a naringin\u00e1zt immobiliz\u00e1lt\u00e1k rajta. Ez a munka adatalapot szolg\u00e1ltat a naringin\u00e1z immobiliz\u00e1ci\u00f3s technol\u00f3giai kutat\u00e1sok tov\u00e1bbi tanulm\u00e1nyoz\u00e1s\u00e1hoz.<\/h4>\r\n\r\n\r\n\r\n

L\u00e9pjen kapcsolatba vel\u00fcnk most!<\/b><\/strong><\/h2>\r\n

Ha sz\u00fcks\u00e9ge van Price-ra, k\u00e9rj\u00fck, t\u00f6ltse ki el\u00e9rhet\u0151s\u00e9g\u00e9t az al\u00e1bbi \u0171rlapon, \u00e1ltal\u00e1ban 24 \u00f3r\u00e1n bel\u00fcl felvessz\u00fck \u00d6nnel a kapcsolatot. \u00d6n is k\u00fcldhet nekem e-mailt\u00a0info@longchangchemical.com<\/a><\/span>\u00a0munkaid\u0151ben ( 8:30-18:00 UTC+8 H.-Szombat ) vagy haszn\u00e1lja a weboldal \u00e9l\u0151 chatj\u00e9t, hogy azonnali v\u00e1laszt kapjon.<\/b><\/strong><\/h4>\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n
\u00d6sszet\u00e9tel Gl\u00fckoamil\u00e1z<\/span><\/a><\/td>\r\n9032-08-0<\/td>\r\n<\/tr>\r\n
Pullulanase<\/span><\/a><\/td>\r\n9075-68-7<\/td>\r\n<\/tr>\r\n
Xilan\u00e1z<\/span><\/a><\/td>\r\n37278-89-0<\/td>\r\n<\/tr>\r\n
Cellul\u00e1z<\/span><\/a><\/td>\r\n9012-54-8<\/td>\r\n<\/tr>\r\n
Naringin\u00e1z<\/span><\/a><\/td>\r\n9068-31-9<\/td>\r\n<\/tr>\r\n
\u03b2-Amil\u00e1z<\/span><\/a><\/td>\r\n9000-91-3<\/td>\r\n<\/tr>\r\n
Gl\u00fck\u00f3z-oxid\u00e1z<\/span><\/a><\/td>\r\n9001-37-0<\/td>\r\n<\/tr>\r\n
alfa-amil\u00e1z<\/td>\r\n9000-90-2<\/td>\r\n<\/tr>\r\n
Pektin\u00e1z<\/span><\/a><\/td>\r\n9032-75-1<\/td>\r\n<\/tr>\r\n
Peroxid\u00e1z<\/td>\r\n9003-99-0<\/td>\r\n<\/tr>\r\n
Lip\u00e1z<\/span><\/a><\/td>\r\n9001-62-1<\/td>\r\n<\/tr>\r\n
Katal\u00e1z<\/span><\/a><\/td>\r\n9001-05-2<\/td>\r\n<\/tr>\r\n
TANNASE<\/span><\/a><\/td>\r\n9025-71-2<\/td>\r\n<\/tr>\r\n
Elaszt\u00e1z<\/span><\/a><\/td>\r\n39445-21-1<\/td>\r\n<\/tr>\r\n
Ure\u00e1z<\/span><\/a><\/td>\r\n9002-13-5<\/td>\r\n<\/tr>\r\n
DEXTRANASE<\/span><\/a><\/td>\r\n9025-70-1<\/td>\r\n<\/tr>\r\n
L-laktil-dehidrogen\u00e1z<\/span><\/a><\/td>\r\n9001-60-9<\/td>\r\n<\/tr>\r\n
Dehidrogen\u00e1z mal\u00e1t<\/span><\/a><\/td>\r\n9001-64-3<\/td>\r\n<\/tr>\r\n
Koleszterin-oxid\u00e1z<\/span><\/a><\/td>\r\n9028-76-6<\/td>\r\n<\/tr>\r\n<\/tbody>\r\n<\/table>","protected":false},"excerpt":{"rendered":"

A m\u00e1gneses biopolimer mikrog\u00f6mb\u00f6k olyan \u00faj kompozit anyagok, amelyek biol\u00f3giai anyagokat \u00e9s szervetlen m\u00e1gneses anyagokat egyes\u00edtenek, hogy m\u00e1gnesesen \u00e9rz\u00e9keny \u00e9s biol\u00f3giailag akt\u00edv mikrog\u00f6mb\u00f6ket alkossanak. Tulajdons\u00e1gai a szervetlen m\u00e1gneses anyagokt\u00f3l, a biol\u00f3giai anyagokt\u00f3l \u00e9s azok k\u00f6lcs\u00f6nhat\u00e1si m\u00f3dszer\u00e9t\u0151l f\u00fcggnek. Jelenleg a legsz\u00e9lesebb k\u00f6rben haszn\u00e1lt \u00e9s vizsg\u00e1lt m\u00e1gneses anyag a Fe3O4 m\u00e1gneses nanor\u00e9szecsk\u00e9k. A nagy fajlagos [...]<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[108],"tags":[],"class_list":["post-4514","post","type-post","status-publish","format-standard","hentry","category-enzyme-news"],"yoast_head":"\nOverview of the Methods of Immobilizing Enzymes on Magnetic Biopolymer Microspheres - Longchang Chemical<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/longchangchemical.com\/hu\/overview-of-the-methods\/\" \/>\n<meta property=\"og:locale\" content=\"hu_HU\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Overview of the Methods of Immobilizing Enzymes on Magnetic Biopolymer Microspheres - Longchang Chemical\" \/>\n<meta property=\"og:description\" content=\"Magnetic biopolymer microspheres are a novel composite material that combines biological materials and inorganic magnetic materials to form magnetically responsive and biologically active microspheres. 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Longchang Chemical","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/longchangchemical.com\/hu\/overview-of-the-methods\/","og_locale":"hu_HU","og_type":"article","og_title":"Overview of the Methods of Immobilizing Enzymes on Magnetic Biopolymer Microspheres - Longchang Chemical","og_description":"Magnetic biopolymer microspheres are a novel composite material that combines biological materials and inorganic magnetic materials to form magnetically responsive and biologically active microspheres. Its properties depend on inorganic magnetic materials, biological materials and their interactions method. At present, the most widely used and studied magnetic material is Fe3O4\u00a0magnetic nanoparticles. 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