{"id":4434,"date":"2021-03-10T13:45:50","date_gmt":"2021-03-10T13:45:50","guid":{"rendered":"https:\/\/longchangchemical.com\/?p=4434"},"modified":"2024-09-28T11:54:34","modified_gmt":"2024-09-28T11:54:34","slug":"summary-of-different-glycoside-hydrolase-reactions","status":"publish","type":"post","link":"https:\/\/longchangchemical.com\/hu\/summary-of-different-glycoside-hydrolase-reactions\/","title":{"rendered":"A k\u00fcl\u00f6nb\u00f6z\u0151 glikozid-hidrol\u00e1z reakci\u00f3k \u00f6sszefoglal\u00e1sa"},"content":{"rendered":"
Jelenleg a glikozid-hidrol\u00e1zokat sz\u00e1mos akt\u00edv glikozid \u00e9s aglikon el\u0151\u00e1ll\u00edt\u00e1s\u00e1nak tanulm\u00e1nyoz\u00e1s\u00e1ra haszn\u00e1lj\u00e1k. K\u00f6z\u00fcl\u00fck a szaponin \u00e9s flavonoid glikozidok enzimatikus el\u0151\u00e1ll\u00edt\u00e1sa oxiglikozidokban a legkiterjedtebb. A tudom\u00e1nyos kutat\u00f3k t\u00f6bb \u00e9ves er\u0151fesz\u00edt\u00e9sei ut\u00e1n a glikozid-hidrol\u00e1z sz\u00e1mos \u00f6r\u00f6mteli eredm\u00e9nyt \u00e9rt el az akt\u00edv glikozidok \u00e9s aglikonok el\u0151\u00e1ll\u00edt\u00e1s\u00e1ban.<\/h5>\r\n\r\n\r\n\r\n

1. A flavonoid glikozidok biotranszform\u00e1ci\u00f3ja<\/h4>\r\n\r\n\r\n\r\n

A flavonoidok a n\u00f6v\u00e9nyekben sz\u00e9les k\u00f6rben elterjedt polifenolok, t\u00f6bbnyire glikozidok form\u00e1j\u00e1ban. Tanulm\u00e1nyok meg\u00e1llap\u00edtott\u00e1k, hogy a biol\u00f3giai aktivit\u00e1s\u00fa flavonoidok az ehet\u0151 n\u00f6v\u00e9nyek legfontosabb hat\u00f3anyagai, \u00e9s t\u00f6bbf\u00e9le farmakol\u00f3giai tev\u00e9kenys\u00e9ggel rendelkeznek, p\u00e9ld\u00e1ul m\u00e1jv\u00e9d\u0151, antioxid\u00e1ci\u00f3s, tumorellenes \u00e9s v\u00edrusellenes hat\u00e1suk van, \u00e9s aktivit\u00e1suk szorosan \u00f6sszef\u00fcgg a szerkezet\u00fckkel. Mivel a legt\u00f6bb flavonoid glikozid nehezen jut be a v\u00e9konyb\u00e9l fal\u00e1n kereszt\u00fcl a v\u00e9rbe, \u00e9s biol\u00f3giai hozz\u00e1f\u00e9rhet\u0151s\u00e9g\u00fck alacsony, a term\u00e9szetes flavonoidok szerkezeti m\u00f3dos\u00edt\u00e1sa a jelenlegi kutat\u00e1sok egyik forr\u00f3 pontj\u00e1v\u00e1 v\u00e1lt. A glikozid-hidrol\u00e1z haszn\u00e1lata a flavonoid-glikozidok glikozilcsoportjainak hidrol\u00edzis\u00e9re hat\u00e9kony m\u00f3dszerr\u00e9 v\u00e1lt a flavonoidok aktivit\u00e1s\u00e1nak jav\u00edt\u00e1s\u00e1ra (1. t\u00e1bl\u00e1zat). A gyakori flavonoid glikozidok k\u00f6z\u00e9 tartozik a rutin, a hesperidin \u00e9s a naringin, \u00e9s cukorr\u00e9szleteik \u00e1ltal\u00e1ban rutin (\u03b1-1,6 kapcsolt ramn\u00f3z \u00e9s gl\u00fck\u00f3z) \u00e9s \u00faj hesper\u00f3z (\u03b1-1,2 kapcsolt ramn\u00f3z \u00e9s gl\u00fck\u00f3z), \u00edgy a glikozid-hidrol\u00e1z hidroliz\u00e1lja a m\u00f3dos\u00edt\u00e1s\u00e1t, amely els\u0151sorban k\u00e9tf\u00e9le exo- \u00e9s endo-kiv\u00e1g\u00e1st tartalmaz. Aspergillus nigerb\u0151l \u00e9s Aspergillus nidulansb\u00f3l izol\u00e1lt\u00e1k \u00e9s tiszt\u00edtott\u00e1k az \u03b1-ramnosid\u00e1zt, amely k\u00e9pes hidroliz\u00e1lni az \u03b1-1,2- \u00e9s \u03b1-1,6-ramnosidk\u00f6t\u00e9seket. Ez az enzim k\u00e9pes hidroliz\u00e1lni a rutint, naringint \u00e9s hesperidint, illetve izoquercetint, plumozidot \u00e9s hesperetin gl\u00fckozidot el\u0151\u00e1ll\u00edtani. Az Aspergillus aculeatusb\u00f3l \u00e9s a Clostridium stercorariumb\u00f3l kl\u00f3nozott rekombin\u00e1ns \u03b1-ramnosid\u00e1z szint\u00e9n rendelkezik a flavonoid glikozidok ramn\u00f3z hidroliz\u00e1l\u00f3 aktivit\u00e1s\u00e1val. A fenti h\u00e1rom flavonoidglikozid hidrol\u00edzis\u00e9hez az exoglikozid\u00e1zok mellett az endoglikozid\u00e1zok is sz\u00e1mos kutat\u00e1si jelent\u00e9ssel rendelkeznek. A Penicillium rugulosumb\u00f3l, Penicillium decumben[ \u00e9s Fagopyri herb\u00e1b\u00f3l izol\u00e1lt \u00e9s tiszt\u00edtott diglikozid\u00e1z, valamint az Aspergillus nigerb\u0151l kl\u00f3nozott rekombin\u00e1ns rutin\u00e1z k\u00e9pes hidroliz\u00e1lni a rutint, hogy jobb antioxid\u00e1ns aktivit\u00e1s\u00fa kvercetin keletkezzen. A naringin\u00e1z izol\u00e1lhat\u00f3 \u00e9s tiszt\u00edthat\u00f3 Aspergillus niger BCC 25166-b\u00f3l, amely k\u00e9pes hidroliz\u00e1lni a naringint naringin el\u0151\u00e1ll\u00edt\u00e1s\u00e1ra. Az Aspergillus niger 1344-ben tal\u00e1lhat\u00f3 naringin\u00e1z egyszerre k\u00e9pes hidroliz\u00e1lni a naringint \u00e9s a rutint naringin el\u0151\u00e1ll\u00edt\u00e1sa c\u00e9lj\u00e1b\u00f3l. Yuan \u00e9s a kvercetin, de a heszperidin nem hidroliz\u00e1lhat\u00f3. Az Acremonium sp. DSM24697-ben \u00e9s az Actinoplanes missouriensis-ben tal\u00e1lhat\u00f3 diglikozid\u00e1z k\u00e9pes hidroliz\u00e1lni a heszperidinben l\u00e9v\u0151 \u00faj heszperidint, hogy rendk\u00edv\u00fcl akt\u00edv heszperetin term\u00e9keket \u00e1ll\u00edtson el\u0151.<\/h5>\r\n\r\n\r\n\r\n

1. t\u00e1bl\u00e1zat. A flavonoid glikozidok biotranszform\u00e1ci\u00f3ja glikozid\u00e1z \u00e1ltal<\/h4>\r\n\r\n\r\n\r\n
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Term\u00e9k<\/td>\r\nAl\u00e1t\u00e9t<\/td>\r\nReakci\u00f3<\/td>\r\nSzervezet<\/td>\r\n<\/tr>\r\n
Isoquercitrin, Prunin,<\/td>\r\nRutin, naringin,<\/td>\r\n\u03b1-Ramnosid\u00e1z<\/td>\r\nAspergillus niger<\/em><\/td>\r\n<\/tr>\r\n
Hesperetin gl\u00fckozid<\/td>\r\nHesperidin<\/td>\r\n\u03b1-Ramnosid\u00e1z<\/td>\r\nAspergillus nidulans<\/em><\/td>\r\n<\/tr>\r\n
\u00a0<\/td>\r\n\u00a0<\/td>\r\n\u03b1-Ramnosid\u00e1z<\/td>\r\nAspergillus aculeatus<\/em><\/td>\r\n<\/tr>\r\n
\u00a0<\/td>\r\n\u00a0<\/td>\r\n\u03b1-Ramnosid\u00e1z<\/td>\r\nClostridium stercorarium<\/em><\/td>\r\n<\/tr>\r\n
Quercetin<\/td>\r\nRutin<\/td>\r\n\u03b2-Rutinosid\u00e1z<\/td>\r\nPenicillium rugulosum<\/em><\/td>\r\n<\/tr>\r\n
Quercetin<\/td>\r\nRutin<\/td>\r\n\u03b2-glikozid\u00e1z<\/td>\r\nPenicillium decumbens<\/em><\/td>\r\n<\/tr>\r\n
Quercetin<\/td>\r\nRutin<\/td>\r\n\u03b2-Heterodiszacharid\u00e1z<\/td>\r\nFagopyri herba<\/em><\/td>\r\n<\/tr>\r\n
Quercetin<\/td>\r\nRutin<\/td>\r\n\u03b2-Rutinosid\u00e1z<\/td>\r\nAspergillus niger<\/em><\/td>\r\n<\/tr>\r\n
Naringenin<\/td>\r\nNaringin<\/td>\r\nNaringin\u00e1z<\/td>\r\nAspergillus niger<\/em><\/td>\r\n<\/tr>\r\n
Naringenin, kvercetin<\/td>\r\nNaringin, rutin<\/td>\r\nNaringin\u00e1z<\/td>\r\nAspergillus niger<\/em><\/td>\r\n<\/tr>\r\n
Hesperetin<\/td>\r\nHesperidin<\/td>\r\nDiglikozid\u00e1z<\/td>\r\nAcremonium<\/em><\/td>\r\n<\/tr>\r\n
Hesperetin<\/td>\r\nHesperidin<\/td>\r\nDiglikozid\u00e1z<\/td>\r\nActinoplanes missouriensis<\/em><\/td>\r\n<\/tr>\r\n
Daidzein<\/td>\r\nDaidzin<\/td>\r\n\u03b2-gl\u00fckozid\u00e1z<\/td>\r\nNem teny\u00e9szthet\u0151 mikrob\u00e1k<\/em><\/td>\r\n<\/tr>\r\n
Daidzein<\/td>\r\nDaidzin<\/td>\r\n\u03b2-gl\u00fckozid\u00e1z<\/td>\r\nSulfolobus solfataricus<\/em><\/td>\r\n<\/tr>\r\n
Daidzein<\/td>\r\nDaidzin<\/td>\r\n\u03b2-gl\u00fckozid\u00e1z<\/td>\r\nAspergillus oryzae<\/em><\/td>\r\n<\/tr>\r\n
Daidzein<\/td>\r\nDaidzin<\/td>\r\n\u03b2-gl\u00fckozid\u00e1z<\/td>\r\nPyrococcus furiosus<\/em><\/td>\r\n<\/tr>\r\n
Daidzein, genisztein<\/td>\r\nDaidzin, Genistin<\/td>\r\n\u03b2-gl\u00fckozid\u00e1z<\/td>\r\nBacillus subtilis<\/em><\/td>\r\n<\/tr>\r\n
\u00a0<\/td>\r\n\u00a0<\/td>\r\n\u03b2-gl\u00fckozid\u00e1z<\/td>\r\nThermotoga maritima<\/em><\/td>\r\n<\/tr>\r\n
Daidzein, genisztein,<\/td>\r\nDaidzin, Genistin,<\/td>\r\n\u03b2-gl\u00fckozid\u00e1z<\/td>\r\nDalbergia<\/em><\/td>\r\n<\/tr>\r\n
Glycitein<\/td>\r\nGlicitin<\/td>\r\n\u03b2-gl\u00fckozid\u00e1z<\/td>\r\nBacteroides thetaiotaomicron<\/em><\/td>\r\n<\/tr>\r\n
Baicalein<\/td>\r\nBaicalin<\/td>\r\n\u03b2-Glikuronid\u00e1z<\/td>\r\nScutellaria viscidula<\/em><\/td>\r\n<\/tr>\r\n
Tilianin<\/td>\r\nLinarin<\/td>\r\nNaringin\u00e1z<\/td>\r\nPenicillium decumbens<\/em><\/td>\r\n<\/tr>\r\n
Butin<\/td>\r\nButrin<\/td>\r\n\u03b2-gl\u00fckozid\u00e1z<\/td>\r\nMandula<\/em><\/td>\r\n<\/tr>\r\n
Phloretin<\/td>\r\nFlorizin<\/td>\r\n\u03b2-glikozid\u00e1z<\/td>\r\nJuh v\u00e9konyb\u00e9l<\/em><\/td>\r\n<\/tr>\r\n<\/tbody>\r\n<\/table>\r\n<\/figure>\r\n\r\n\r\n\r\n
Az izoflavonok egyfajta flavonoidok, amelyek f\u0151k\u00e9nt a h\u00fcvelyesekben tal\u00e1lhat\u00f3k, \u00e9s hozz\u00e1j\u00e1rulnak a betegs\u00e9gek megel\u0151z\u00e9s\u00e9hez \u00e9s az emberi eg\u00e9szs\u00e9ghez. A sz\u00f3ja izoflavonok f\u0151 \u00f6sszetev\u0151i a daidzein, daidzein, genisztin, genistein, glicitein \u00e9s glicitein aglikon, amelyek k\u00f6z\u00fcl a deglikozil\u00e1lt aglikon jobb biol\u00f3giai aktivit\u00e1ssal rendelkezik. A rekombin\u00e1ns \u03b2-gl\u00fckozid\u00e1zt, amely k\u00e9pes hidroliz\u00e1lni a daidzeint daidzein el\u0151\u00e1ll\u00edt\u00e1s\u00e1ra, mangrove talaj, Sulfolobus solfataricus, Aspergillus oryzae \u00e9s Pyrococcus furiosus g\u00e9nk\u00f6nyvt\u00e1r\u00e1b\u00f3l kl\u00f3nozt\u00e1k; a Thermotoga maritima \u00e9s Bacillus subtilis enzimekb\u0151l kl\u00f3nozott rekombin\u00e1ns \u03b2-gl\u00fckozid\u00e1z k\u00e9pes hidroliz\u00e1lni a daidzeint \u00e9s a genisteint daidzein \u00e9s genistein el\u0151\u00e1ll\u00edt\u00e1s\u00e1ra; a Dalbergia-b\u00f3l izol\u00e1lt \u00e9s tiszt\u00edtott, valamint a Bacteroides thetaiotaomicronb\u00f3l kl\u00f3nozott \u00e9s rekombin\u00e1lt glikozid\u00e1z k\u00e9pes hidroliz\u00e1lni a daidzeint, genisztint \u00e9s daidzeint daidzein, genisztein \u00e9s glicitein aglikon el\u0151\u00e1ll\u00edt\u00e1s\u00e1ra.<\/h5>\r\n\r\n\r\n\r\n
A glikozid-hidrol\u00e1zr\u00f3l m\u00e1s flavonoid-glikozidok hidrol\u00edzis\u00e9ben is besz\u00e1moltak \u00e9s alkalmazt\u00e1k (1. t\u00e1bl\u00e1zat). Vizsg\u00e1latok kimutatt\u00e1k, hogy a baicalin tumorellenes \u00e9s fert\u0151z\u00e9sellenes hat\u00e1ssal rendelkezik. A Scutellaria viscidula Bge-b\u0151l izol\u00e1lt \u00e9s tiszt\u00edtott \u03b2-gl\u00fckozid\u00e1z k\u00e9pes hidroliz\u00e1lni a baicalint baicalein el\u0151\u00e1ll\u00edt\u00e1s\u00e1ra. A deglikozil\u00e1lt term\u00e9k, a baicalein jobb farmakol\u00f3giai aktivit\u00e1ssal rendelkezik. A szerimarin szint\u00e9n ritka flavonoid glikozid, amely v\u00e9rnyom\u00e1scs\u00f6kkent\u0151 \u00e9s nyugtat\u00f3 hat\u00e1s\u00fa, de k\u00f6zvetlen extrakci\u00f3val \u00e9s k\u00e9miai szint\u00e9zissel nehezen nyerhet\u0151. Cui \u00e9s munkat\u00e1rsai naringin\u00e1zt haszn\u00e1ltak a montanozidban l\u00e9v\u0151 ramn\u00f3z hidroliz\u00e1l\u00e1s\u00e1hoz a szerimarin el\u0151\u00e1ll\u00edt\u00e1s\u00e1hoz. Ezenk\u00edv\u00fcl Jassbi et al. \u03b2-gl\u00fckozid\u00e1zt haszn\u00e1lt a butrin hidroliz\u00e1l\u00e1s\u00e1hoz butrin el\u0151\u00e1ll\u00edt\u00e1s\u00e1hoz. Az antioxid\u00e1ns k\u00eds\u00e9rletek eredm\u00e9nyei azt mutatt\u00e1k, hogy a deglikozil\u00e1lt butrin jobb aktivit\u00e1ssal rendelkezett, mint a butrin. Day \u00e9s m\u00e1s, juhok v\u00e9konybel\u00e9b\u0151l izol\u00e1lt \u00e9s tiszt\u00edtott glikozid\u00e1zok k\u00e9pesek a florizin hidroliz\u00e1l\u00e1s\u00e1ra, hogy floretint \u00e1ll\u00edtsanak el\u0151.<\/h5>\r\n\r\n\r\n\r\n

2. Egy\u00e9b oxiglikozidok biotranszform\u00e1ci\u00f3ja<\/h4>\r\n\r\n\r\n\r\n

A szaponinok \u00e9s flavonoid glikozidok mellett a glikozid hidrol\u00e1zokat m\u00e1s oxig\u00e9nglikozidok hidroliz\u00e1l\u00e1s\u00e1ra \u00e9s m\u00f3dos\u00edt\u00e1s\u00e1ra is haszn\u00e1lt\u00e1k (2. t\u00e1bl\u00e1zat). A gard\u00e9nia gy\u00fcm\u00f6lcse hagyom\u00e1nyos k\u00ednai gy\u00f3gy\u00e1szatban sz\u00edv- \u00e9s \u00e9rrendszeri, agyi \u00e9rrendszeri, m\u00e1j- \u00e9s epeh\u00f3lyagbetegs\u00e9gek kezel\u00e9s\u00e9re haszn\u00e1lj\u00e1k. A gardenia gy\u00fcm\u00f6lcs\u00f6kben sok genipozid van, de a hat\u00e9kony \u00f6sszetev\u0151 a genipozid, amely a genipozid deglikozil\u00e1lt term\u00e9ke, \u00e9s a tartalma kevesebb, mint 0,01%. A Penicillium nigricansb\u00f3l \u00e9s Aspergillus nigerb\u0151l izol\u00e1lt \u00e9s tiszt\u00edtott \u03b2-gl\u00fckozid\u00e1z genipozidd\u00e1 alak\u00edthat\u00f3 a genipin el\u0151\u00e1ll\u00edt\u00e1s\u00e1hoz, hogy kiel\u00e9g\u00edtse a nagy mennyis\u00e9g\u0171 genipin ir\u00e1nti ig\u00e9nyt. Az Arctium lappa a kr\u00f3nikus veseel\u00e9gtelens\u00e9g megel\u0151z\u00e9s\u00e9re vagy kezel\u00e9s\u00e9re alkalmas, \u00e9s hat\u00e9kony \u00f6sszetev\u0151i az arctiin \u00e9s az arctigenin. A Grifola frondosa \u00e9s a Rhizoctonia solani \u03b2-gl\u00fckozid\u00e1za k\u00e9pes a bojtorj\u00e1nt arctigenin el\u0151\u00e1ll\u00edt\u00e1s\u00e1ra \u00e1talak\u00edtani. Liu \u00e9s munkat\u00e1rsai kereskedelmi \u03b2-gl\u00fckozid\u00e1zt haszn\u00e1ltak a bojtorj\u00e1n term\u00e9s\u00e9nek teljes hidrol\u00edzis\u00e9hez, hogy a bojtorj\u00e1n aglikon term\u00e9ket nyerj\u00e9k. Az arctiin arctigeninn\u00e9 t\u00f6rt\u00e9n\u0151 \u00e1talak\u00edt\u00e1sa hat\u00e9konyan jav\u00edthatja a biol\u00f3giai hozz\u00e1f\u00e9rhet\u0151s\u00e9get. A rezveratrolnak a daganatok \u00e9s az \u00e9relmeszesed\u00e9s megel\u0151z\u00e9s\u00e9nek funkci\u00f3i vannak. Az Aspergillus oryzae sp. 100-b\u00f3l \u00e9s Lactobacillus kimchib\u0151l izol\u00e1lt \u00e9s tiszt\u00edtott \u03b2-gl\u00fckozid\u00e1z, valamint a Mai \u00e9s munkat\u00e1rsai \u00e1ltal mangrove talaj metagenomik\u00e1j\u00e1b\u00f3l kl\u00f3nozott rekombin\u00e1ns \u03b2-gl\u00fckozid\u00e1z k\u00e9pes a polydatint rezveratrol el\u0151\u00e1ll\u00edt\u00e1s\u00e1ra. A paclitaxel a Taxus chinensis n\u00f6v\u00e9ny m\u00e1sodlagos metabolitja, \u00e9s j\u00f3 ter\u00e1pi\u00e1s hat\u00e1ssal van a petef\u00e9szekr\u00e1kra \u00e9s az eml\u0151r\u00e1kra. A paclitaxel sz\u00e1raz t\u00f6mege a Taxus chinensisben mind\u00f6ssze 0,02%, \u00e9s a hullad\u00e9kk\u00e9nt kidobott 7-xil\u00f3z-10-deacetil-paclitaxel tartalma t\u00f6bb mint 10-szerese a paclitaxelnek. Dou \u00e9s munkat\u00e1rsai a Cellulosimicrobium cellulans F16 t\u00f6rzs \u00e1ltal szekret\u00e1lt extracellul\u00e1ris xilozid\u00e1zt haszn\u00e1lt\u00e1k a 7-xil\u00f3z-10-deacetil-paclitaxel 10-deacetil-paclitaxell\u00e9 t\u00f6rt\u00e9n\u0151 \u00e1talak\u00edt\u00e1s\u00e1ra, majd egyl\u00e9p\u00e9ses acil\u00e1l\u00e1si reakci\u00f3val paclitaxelt hoztak l\u00e9tre.<\/h5>\r\n\r\n\r\n\r\n

2. t\u00e1bl\u00e1zat. Egy\u00e9b O-glikozidok biotranszform\u00e1ci\u00f3ja glikozid\u00e1z \u00e1ltal<\/h4>\r\n\r\n\r\n\r\n
\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n
Term\u00e9k<\/td>\r\nAl\u00e1t\u00e9t<\/td>\r\nReakci\u00f3<\/td>\r\nSzervezet<\/td>\r\n<\/tr>\r\n
Genipin<\/td>\r\nGenipozid<\/td>\r\n\u03b2-gl\u00fckozid\u00e1z<\/td>\r\nPenicillium<\/em>\u00a0<\/em>nigricans<\/em><\/td>\r\n<\/tr>\r\n
Genipin<\/td>\r\nGenipozid<\/td>\r\n\u03b2-gl\u00fckozid\u00e1z<\/td>\r\nAspergillus<\/em>\u00a0<\/em>niger<\/em><\/td>\r\n<\/tr>\r\n
Arctigenin<\/td>\r\nArctiin<\/td>\r\n\u03b2-gl\u00fckozid\u00e1z<\/td>\r\nGrifola<\/em>\u00a0<\/em>frondosa<\/em><\/td>\r\n<\/tr>\r\n
Arctigenin<\/td>\r\nArctiin<\/td>\r\n\u03b2-gl\u00fckozid\u00e1z<\/td>\r\nRhizoctonia<\/em>\u00a0<\/em>solani<\/em><\/td>\r\n<\/tr>\r\n
Arctigenin<\/td>\r\nArctiin<\/td>\r\n\u03b2-gl\u00fckozid\u00e1z<\/td>\r\nKereskedelmi<\/td>\r\n<\/tr>\r\n
Resveratrol<\/td>\r\nPolydatin<\/td>\r\n\u03b2-gl\u00fckozid\u00e1z<\/td>\r\nAspergillus<\/em>\u00a0<\/em>oryzae<\/em><\/td>\r\n<\/tr>\r\n
Resveratrol<\/td>\r\nPolydatin<\/td>\r\n\u03b2-gl\u00fckozid\u00e1z<\/td>\r\nLactobacillus<\/em>\u00a0<\/em>kimchi<\/em><\/td>\r\n<\/tr>\r\n
Resveratrol<\/td>\r\nPolydatin<\/td>\r\n\u03b2-gl\u00fckozid\u00e1z<\/td>\r\nNem teny\u00e9szthet\u0151 mikrob\u00e1k<\/td>\r\n<\/tr>\r\n
10-Deacetil-paclitaxel<\/td>\r\n7-Xilozil-10-deacetil-paklitaxel<\/td>\r\n\u03b2-Xilozid\u00e1z<\/td>\r\nCellulosimicrobium<\/em>\u00a0<\/em>cellul\u00e1n<\/em>s<\/td>\r\n<\/tr>\r\n<\/tbody>\r\n<\/table>\r\n<\/figure>\r\n\r\n\r\n\r\n

3. Sz\u00e9nglikozidok \u00e9s tioglikozidok biotranszform\u00e1ci\u00f3ja<\/h4>\r\n\r\n\r\n\r\n

Az oxiglikozidok mellett a glikozid-hidrol\u00e1zokat is haszn\u00e1lj\u00e1k a sz\u00e9nglikozidok \u00e9s a tioglikozidok hidrol\u00edzism\u00f3dos\u00edt\u00e1s\u00e1nak vizsg\u00e1lat\u00e1ra (3. t\u00e1bl\u00e1zat). A sz\u00e9nglikozidok az aglikon fenolos hidroxilcsoportja \u00e1ltal aktiv\u00e1lt orto- vagy para-poz\u00edci\u00f3s hidrog\u00e9nnek cukorcsoportokkal t\u00f6rt\u00e9n\u0151 dehidrat\u00e1ci\u00f3s kondenz\u00e1ci\u00f3j\u00e1val j\u00f6nnek l\u00e9tre. A karbozid flavonoidok sz\u00e1mos tev\u00e9kenys\u00e9ggel rendelkeznek, p\u00e9ld\u00e1ul gyullad\u00e1scs\u00f6kkent\u0151, antibakteri\u00e1lis, daganatellenes, v\u00e9rcukorszint-cs\u00f6kkent\u0151 \u00e9s immuner\u0151s\u00edt\u0151 hat\u00e1s\u00faak. Az oxozid flavonoidokkal \u00f6sszehasonl\u00edtva a karbozid flavonoidok nagyobb stabilit\u00e1ssal rendelkeznek, teljesen felsz\u00edv\u00f3dhatnak \u00e9s potenci\u00e1lis gy\u00f3gyszermolekul\u00e1kk\u00e1 v\u00e1lhatnak. Mivel a sz\u00e9nglikozidos k\u00f6t\u00e9sek nehezen hidroliz\u00e1lhat\u00f3k, a sz\u00e9nglikozid flavonoidok hidrol\u00edzis\u00e9r\u0151l kev\u00e9s jelent\u00e9s sz\u00fcletett. Sanugul \u00e9s munkat\u00e1rsai emberi sz\u00e9kletbakt\u00e9riumok kever\u00e9k\u00e9b\u0151l izol\u00e1ltak egy bakt\u00e9riumot, amely a mangiferin indukci\u00f3ja alatt egy glikozid\u00e1zt szekret\u00e1lt, amely k\u00e9pes hidroliz\u00e1lni a mangiferinben l\u00e9v\u0151 sz\u00e9nglikozidos k\u00f6t\u00e9seket, hogy jobb aktivit\u00e1s\u00fa mangiferint \u00e1ll\u00edtson el\u0151. Nakamura \u00e9s munkat\u00e1rsai emberi b\u00e9lbakt\u00e9riumokb\u00f3l izol\u00e1lt\u00e1k a PUE t\u00f6rzset, amely k\u00e9pes izol\u00e1lni \u00e9s tiszt\u00edtani egy karboglikozid\u00e1zt, amely a puerarint hidroliz\u00e1lja aglikon el\u0151\u00e1ll\u00edt\u00e1s\u00e1ra. A karboglikozid\u00e1zt k\u00f3dol\u00f3 g\u00e9nek vizsg\u00e1lata sor\u00e1n Braune \u00e9s munkat\u00e1rsai meg\u00e1llap\u00edtott\u00e1k, hogy az Eubacterium cellulosolvensben tal\u00e1lhat\u00f3 dfgA, dfgB, dfgC, dfgD \u00e9s dfgE feh\u00e9rjek\u00f3dol\u00f3 g\u00e9nek egy\u00fcttesen olyan karboglikozid\u00e1zokat fejeznek ki, amelyek k\u00e9pesek hidroliz\u00e1lni az izoorientint a megfelel\u0151 aglikonok el\u0151\u00e1ll\u00edt\u00e1sa \u00e9rdek\u00e9ben.<\/h5>\r\n\r\n\r\n\r\n
A gl\u00fckozinol\u00e1tok a gl\u00fckozinol\u00e1t vegy\u00fcletek egyik fontos oszt\u00e1lya, amelyek sz\u00e9les k\u00f6rben megtal\u00e1lhat\u00f3k a keresztesvir\u00e1g\u00fa n\u00f6v\u00e9nyekben, p\u00e9ld\u00e1ul a must\u00e1rban, a brokkoliban, a fokhagym\u00e1ban stb. Tanulm\u00e1nyok kimutatt\u00e1k, hogy a keresztesvir\u00e1g\u00fa n\u00f6v\u00e9nyek fogyaszt\u00e1sa hat\u00e9konyan megel\u0151zheti a mellr\u00e1kot, a t\u00fcd\u0151r\u00e1kot, a vastagb\u00e9lr\u00e1kot \u00e9s m\u00e1s r\u00e1kos megbeteged\u00e9seket. A f\u0151 hat\u00f3anyag az izotiocian\u00e1t, amely a gl\u00fckozinol\u00e1tok leboml\u00e1sa ut\u00e1n keletkezik. A gl\u00fckozid\u00e1z, m\u00e1s n\u00e9ven mirozin\u00e1z f\u0151k\u00e9nt a keresztesvir\u00e1g\u00fa n\u00f6v\u00e9nyekben tal\u00e1lhat\u00f3 meg, de a gl\u00fckozinol\u00e1tokt\u00f3l elt\u00e9r\u0151 helyen oszlik el. Csak akkor keverednek \u00e9s l\u00e9pnek reakci\u00f3ba, amikor a sejtek felbomlanak. Az endog\u00e9n mirozin\u00e1z alacsony tartalma miatt neh\u00e9z a gl\u00fckozinol\u00e1tokat hat\u00e9konyan hidroliz\u00e1lni, hogy akt\u00edv term\u00e9keket \u00e1ll\u00edtsanak el\u0151. A szulforaf\u00e1n egy farmakol\u00f3giai aktivit\u00e1ssal rendelkez\u0151 izotiocian\u00e1t. Shen \u00e9s munkat\u00e1rsai exog\u00e9n mirozin\u00e1zt haszn\u00e1ltak a gl\u00fckoraf\u00e1nin sikeres \u00e1talak\u00edt\u00e1s\u00e1ra szulforaf\u00e1nn\u00e1. Jelenleg kev\u00e9s tanulm\u00e1ny sz\u00fcletett a sz\u00e9nglikozidok \u00e9s tioglikozidok hidrolitikus m\u00f3dos\u00edt\u00e1s\u00e1val kapcsolatban. A j\u00f6v\u0151ben t\u00f6bb sz\u00e9nglikozid \u00e9s tioglikozid fejleszt\u00e9se \u00e9s szerkezeti m\u00f3dos\u00edt\u00e1sa t\u00f6bb gy\u00f3gyszerfejleszt\u00e9sre alkalmas molekul\u00e1t fog biztos\u00edtani.<\/h5>\r\n\r\n\r\n\r\n

3. t\u00e1bl\u00e1zat. A C-glikozidok \u00e9s S-glikozidok glikozid\u00e1z \u00e1ltali biotranszform\u00e1ci\u00f3ja.<\/h4>\r\n\r\n\r\n\r\n
\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n
Term\u00e9k<\/td>\r\nAl\u00e1t\u00e9t<\/td>\r\nReakci\u00f3<\/td>\r\nSzervezet<\/td>\r\n<\/tr>\r\n
Norathyriol<\/td>\r\nMangiferin<\/td>\r\nC-gl\u00fckozil-bont\u00f3 enzim<\/td>\r\nBacteroides<\/em><\/td>\r\n<\/tr>\r\n
Daidzein<\/td>\r\nPuerarin<\/td>\r\nC-gl\u00fckozil-bont\u00f3 enzim<\/td>\r\nEmberi<\/em>\u00a0<\/em>b\u00e9lrendszeri<\/em>\u00a0<\/em>bakt\u00e9rium<\/em><\/td>\r\n<\/tr>\r\n
Luteolin<\/td>\r\nHomoorientin<\/td>\r\nC-gl\u00fckozil-bont\u00f3 enzim<\/td>\r\nEubacterium<\/em>\u00a0<\/em>celluloszolvens<\/em><\/td>\r\n<\/tr>\r\n
Szulforaf\u00e1n<\/td>\r\nGl\u00fckorafanin<\/td>\r\nMyrosinase<\/td>\r\nBrokkoli magok<\/em><\/td>\r\n<\/tr>\r\n<\/tbody>\r\n<\/table>\r\n<\/figure>\r\n\r\n\r\n\r\n

L\u00e9pjen kapcsolatba vel\u00fcnk most!<\/b><\/strong><\/h2>\r\n

Ha sz\u00fcks\u00e9ge van Price-ra, k\u00e9rj\u00fck, t\u00f6ltse ki el\u00e9rhet\u0151s\u00e9g\u00e9t az al\u00e1bbi \u0171rlapon, \u00e1ltal\u00e1ban 24 \u00f3r\u00e1n bel\u00fcl felvessz\u00fck \u00d6nnel a kapcsolatot. \u00d6n is k\u00fcldhet nekem e-mailt\u00a0info@longchangchemical.com<\/a><\/span>\u00a0munkaid\u0151ben ( 8:30-18:00 UTC+8 H.-Szombat ) vagy haszn\u00e1lja a weboldal \u00e9l\u0151 chatj\u00e9t, hogy azonnali v\u00e1laszt kapjon.<\/b><\/strong><\/h4>\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n
\u00d6sszet\u00e9tel Gl\u00fckoamil\u00e1z<\/span><\/a><\/td>\r\n9032-08-0<\/td>\r\n<\/tr>\r\n
Pullulanase<\/span><\/a><\/td>\r\n9075-68-7<\/td>\r\n<\/tr>\r\n
Xilan\u00e1z<\/span><\/a><\/td>\r\n37278-89-0<\/td>\r\n<\/tr>\r\n
Cellul\u00e1z<\/span><\/a><\/td>\r\n9012-54-8<\/td>\r\n<\/tr>\r\n
Naringin\u00e1z<\/span><\/a><\/td>\r\n9068-31-9<\/td>\r\n<\/tr>\r\n
\u03b2-Amil\u00e1z<\/span><\/a><\/td>\r\n9000-91-3<\/td>\r\n<\/tr>\r\n
Gl\u00fck\u00f3z-oxid\u00e1z<\/span><\/a><\/td>\r\n9001-37-0<\/td>\r\n<\/tr>\r\n
alfa-amil\u00e1z<\/td>\r\n9000-90-2<\/td>\r\n<\/tr>\r\n
Pektin\u00e1z<\/span><\/a><\/td>\r\n9032-75-1<\/td>\r\n<\/tr>\r\n
Peroxid\u00e1z<\/td>\r\n9003-99-0<\/td>\r\n<\/tr>\r\n
Lip\u00e1z<\/span><\/a><\/td>\r\n9001-62-1<\/td>\r\n<\/tr>\r\n
Katal\u00e1z<\/span><\/a><\/td>\r\n9001-05-2<\/td>\r\n<\/tr>\r\n
TANNASE<\/span><\/a><\/td>\r\n9025-71-2<\/td>\r\n<\/tr>\r\n
Elaszt\u00e1z<\/span><\/a><\/td>\r\n39445-21-1<\/td>\r\n<\/tr>\r\n
Ure\u00e1z<\/span><\/a><\/td>\r\n9002-13-5<\/td>\r\n<\/tr>\r\n
DEXTRANASE<\/span><\/a><\/td>\r\n9025-70-1<\/td>\r\n<\/tr>\r\n
L-laktil-dehidrogen\u00e1z<\/span><\/a><\/td>\r\n9001-60-9<\/td>\r\n<\/tr>\r\n
Dehidrogen\u00e1z mal\u00e1t<\/span><\/a><\/td>\r\n9001-64-3<\/td>\r\n<\/tr>\r\n
Koleszterin-oxid\u00e1z<\/span><\/a><\/td>\r\n9028-76-6<\/td>\r\n<\/tr>\r\n<\/tbody>\r\n<\/table>","protected":false},"excerpt":{"rendered":"

At present, glycoside hydrolases are used in the study of the preparation of a variety of active glycosides and aglycons. Among them, the enzymatic preparation of saponin and flavonoid glycosides in oxyglycosides is the most extensive. After years of efforts by scientific researchers, glycoside hydrolase has achieved many gratifying results in the preparation of active […]<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[108],"tags":[],"class_list":["post-4434","post","type-post","status-publish","format-standard","hentry","category-enzyme-news"],"yoast_head":"\nSummary of different glycoside hydrolase reactions - Longchang Chemical Co., Ltd.<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/longchangchemical.com\/hu\/summary-of-different-glycoside-hydrolase-reactions\/\" \/>\n<meta property=\"og:locale\" content=\"hu_HU\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Summary of different glycoside hydrolase reactions - Longchang Chemical Co., Ltd.\" \/>\n<meta property=\"og:description\" content=\"At present, glycoside hydrolases are used in the study of the preparation of a variety of active glycosides and aglycons. 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