{"id":5451,"date":"2022-08-12T12:25:35","date_gmt":"2022-08-12T12:25:35","guid":{"rendered":"https:\/\/longchangchemical.com\/?p=5451"},"modified":"2022-08-12T12:25:35","modified_gmt":"2022-08-12T12:25:35","slug":"introduction-and-principles-of-common-laboratory-instrument-analysis","status":"publish","type":"post","link":"https:\/\/longchangchemical.com\/fr\/introduction-and-principles-of-common-laboratory-instrument-analysis\/","title":{"rendered":"Introduction et principes de l'analyse des instruments de laboratoire courants"},"content":{"rendered":"

Introduction et principes de l'analyse des instruments de laboratoire courants<\/strong><\/h1>\n

1. spectrom\u00e8tre d'absorption infrarouge, IR<\/p>\n

Principe analytique : absorption de l'\u00e9nergie de la lumi\u00e8re infrarouge, provoquant des sauts de niveau d'\u00e9nergie de vibration et de rotation des mol\u00e9cules avec des moments dipolaires changeants.<\/p>\n

Repr\u00e9sentation du spectre : variation de l'\u00e9nergie lumineuse transmise relative en fonction de la fr\u00e9quence de la lumi\u00e8re transmise.<\/p>\n

Informations fournies : emplacement, intensit\u00e9 et forme des pics, fournissant les fr\u00e9quences vibratoires caract\u00e9ristiques des groupes fonctionnels ou des liaisons chimiques.<\/p>\n

2. Spectrom\u00e8tre d'absorption dans l'ultraviolet, UV<\/p>\n

Principe de l'analyse : absorption de l'\u00e9nergie UV, provoquant un saut dans le niveau d'\u00e9nergie des \u00e9lectrons de la mol\u00e9cule.<\/p>\n

Repr\u00e9sentation du spectre : variation de l'\u00e9nergie lumineuse relative absorb\u00e9e en fonction de la longueur d'onde de la lumi\u00e8re absorb\u00e9e.<\/p>\n

Informations fournies : emplacement, intensit\u00e9 et forme des pics d'absorption, fournissant des informations sur les diff\u00e9rentes structures \u00e9lectroniques de la mol\u00e9cule.<\/p>\n

3. Spectrom\u00e9trie de r\u00e9sonance magn\u00e9tique nucl\u00e9aire, RMN<\/p>\n

Principe de l'analyse : des noyaux avec des moments magn\u00e9tiques nucl\u00e9aires dans un champ magn\u00e9tique externe, absorbant l'\u00e9nergie des radiofr\u00e9quences et produisant des sauts dans les niveaux d'\u00e9nergie du spin nucl\u00e9aire.<\/p>\n

Repr\u00e9sentation du spectre : variation de l'\u00e9nergie lumineuse absorb\u00e9e en fonction du d\u00e9placement chimique.<\/p>\n

Informations fournies : d\u00e9placements chimiques, intensit\u00e9s, fractions de clivage et constantes de couplage des pics, fournissant des informations sur le nombre de noyaux, l'environnement chimique dans lequel ils se trouvent et leur configuration g\u00e9om\u00e9trique.<\/p>\n

4. Spectrom\u00e8tre de fluorescence, FS.<\/p>\n

Principe de l'analyse : \u00e9mission de fluorescence apr\u00e8s excitation par un rayonnement \u00e9lectromagn\u00e9tique, de l'\u00e9tat excit\u00e9 *faible \u00e0 une ligne \u00e0 l'\u00e9tat fondamental \u00e0 une ligne.<\/p>\n

Repr\u00e9sentation du spectre : variation de l'\u00e9nergie de fluorescence \u00e9mise avec la longueur d'onde de la lumi\u00e8re.<\/p>\n

Informations fournies : efficacit\u00e9 et dur\u00e9e de vie de la fluorescence, fournissant des informations sur les diff\u00e9rentes structures \u00e9lectroniques de la mol\u00e9cule.<\/p>\n

5. Spectrom\u00e8tre Raman, Ram.<\/p>\n

Principe de l'analyse : l'absorption de l'\u00e9nergie lumineuse provoque des vibrations des mol\u00e9cules avec un changement du taux de polarisation, produisant une diffusion Raman.<\/p>\n

Repr\u00e9sentation du spectre : variation de l'\u00e9nergie de la lumi\u00e8re diffus\u00e9e avec le d\u00e9calage Raman.<\/p>\n

Informations fournies : emplacement, intensit\u00e9 et forme des pics, fournissant les fr\u00e9quences vibratoires caract\u00e9ristiques des groupes fonctionnels ou des liaisons chimiques.<\/p>\n

6. Analyseur de spectrom\u00e9trie de masse, MS.<\/p>\n

Principe analytique : les mol\u00e9cules sont bombard\u00e9es d'\u00e9lectrons dans le vide, formant des ions, qui sont s\u00e9par\u00e9s par des champs \u00e9lectromagn\u00e9tiques \u00e0 diff\u00e9rents m\/e.<\/p>\n

Repr\u00e9sentation du spectre : l'aplatissement relatif des ions sous forme de graphique \u00e0 barres avec m\/e.<\/p>\n

Informations fournies : nombre de masse des ions mol\u00e9culaires et des ions de fragments et leur kurtosis relatif, fournissant des informations sur le poids mol\u00e9culaire, la composition \u00e9l\u00e9mentaire et la structure.<\/p>\n

7. Chromatographie en phase gazeuse, GC.<\/p>\n

Principe de l'analyse : s\u00e9paration des composants de l'\u00e9chantillon entre la phase mobile et la phase stationnaire, en raison de coefficients de partage diff\u00e9rents.<\/p>\n

Repr\u00e9sentation du spectre : variation de la concentration de l'effluent post-colonne en fonction de la valeur de r\u00e9tention.<\/p>\n

Informations fournies : la valeur de r\u00e9tention du pic est li\u00e9e aux param\u00e8tres thermodynamiques des composants et constitue la base qualitative ; la surface du pic est li\u00e9e \u00e0 la teneur en composants.<\/p>\n

8. spectrom\u00e9trie de r\u00e9sonance paramagn\u00e9tique \u00e9lectronique, ESR.<\/p>\n

Principe analytique : absorption de l'\u00e9nergie des radiofr\u00e9quences par des \u00e9lectrons non appari\u00e9s dans des mol\u00e9cules soumises \u00e0 un champ magn\u00e9tique externe, ce qui entra\u00eene des sauts de niveau d'\u00e9nergie du spin des \u00e9lectrons.<\/p>\n

Repr\u00e9sentation du spectre : variation de l'\u00e9nergie lumineuse absorb\u00e9e ou de l'\u00e9nergie diff\u00e9rentielle en fonction de l'intensit\u00e9 du champ magn\u00e9tique.<\/p>\n

Informations fournies : positions des lignes spectrales, intensit\u00e9s, nombre de clivages et constantes de division hyperfine, fournissant des informations sur les densit\u00e9s d'\u00e9lectrons non appari\u00e9s, les propri\u00e9t\u00e9s de liaison mol\u00e9culaire et les configurations g\u00e9om\u00e9triques.<\/p>\n

9. Chromatographe \u00e0 gaz de clivage, PGC.<\/p>\n

Principe analytique : clivage instantan\u00e9 de mat\u00e9riaux polym\u00e8res dans certaines conditions pour obtenir des fragments pr\u00e9sentant certaines caract\u00e9ristiques.<\/p>\n

Repr\u00e9sentation du spectre : variation de la concentration de l'effluent post-colonne en fonction de la valeur de r\u00e9tention.<\/p>\n

Informations fournies : empreinte du spectre ou pics de fragmentation caract\u00e9ristiques, caract\u00e9risant la structure chimique et la configuration g\u00e9om\u00e9trique du polym\u00e8re.<\/p>\n

10 . Chromatographie sur gel, GPC.<\/p>\n

Principe de l'analyse : s\u00e9paration de l'\u00e9chantillon \u00e0 travers la colonne de gel en fonction du volume hydrodynamique des mol\u00e9cules, les plus grosses mol\u00e9cules s'\u00e9coulant en premier.<\/p>\n

Repr\u00e9sentation du spectre : variation de la concentration de l'effluent post-colonne en fonction de la valeur de r\u00e9tention.<\/p>\n

Informations fournies : le poids mol\u00e9culaire moyen des polym\u00e8res et leur r\u00e9partition.<\/p>\n

11. Chromatographie invers\u00e9e en phase gazeuse, IGC.<\/p>\n

Principe analytique : variation de la valeur de r\u00e9tention de la mol\u00e9cule sonde en fonction des forces d'interaction entre celle-ci et l'\u00e9chantillon de polym\u00e8re servant de phase stationnaire.<\/p>\n

Repr\u00e9sentation du spectre : courbe de la variation du logarithme du volume de r\u00e9tention sp\u00e9cifique de la mol\u00e9cule sonde avec l'inverse de la temp\u00e9rature de la colonne.<\/p>\n

Informations fournies : la valeur de r\u00e9tention de la mol\u00e9cule sonde en fonction de la temp\u00e9rature fournit les param\u00e8tres thermodynamiques du polym\u00e8re.<\/p>\n

12. Thermogravim\u00e9trie, TG.<\/p>\n

Principe de l'analyse : variation du poids de l'\u00e9chantillon en fonction de la temp\u00e9rature ou du temps dans un environnement \u00e0 temp\u00e9rature contr\u00f4l\u00e9e.<\/p>\n

Repr\u00e9sentation du spectre : courbe de la fraction pond\u00e9rale de l'\u00e9chantillon en fonction de la temp\u00e9rature ou du temps.<\/p>\n

Informations fournies : la chute abrupte de la courbe correspond \u00e0 la zone de perte de poids de l'\u00e9chantillon, et la zone de plateau correspond \u00e0 la zone de stabilit\u00e9 thermique de l'\u00e9chantillon.<\/p>\n

13. analyseur statique de force thermique, TMA.<\/p>\n

Principe de l'analyse : d\u00e9formation de l'\u00e9chantillon sous l'action d'une force constante en fonction de la temp\u00e9rature ou du temps.<\/p>\n

Repr\u00e9sentation du spectre : courbe des valeurs de d\u00e9formation de l'\u00e9chantillon en fonction de la temp\u00e9rature ou du temps.<\/p>\n

Informations fournies : temp\u00e9rature de transition thermique et \u00e9tat m\u00e9canique.<\/p>\n

14. Analyseur thermique diff\u00e9rentiel, DTA.<\/p>\n

Principe analytique : l'\u00e9chantillon et la r\u00e9f\u00e9rence sont plac\u00e9s dans le m\u00eame environnement \u00e0 temp\u00e9rature contr\u00f4l\u00e9e, et la diff\u00e9rence de temp\u00e9rature est g\u00e9n\u00e9r\u00e9e par la conductivit\u00e9 thermique diff\u00e9rente des deux, et le changement de temp\u00e9rature en fonction de la temp\u00e9rature ambiante ou du temps est enregistr\u00e9.<\/p>\n

Repr\u00e9sentation du spectre : courbe de la diff\u00e9rence de temp\u00e9rature en fonction de la temp\u00e9rature ambiante ou du temps.<\/p>\n

Informations fournies : fournir des informations sur la temp\u00e9rature de transition thermique du polym\u00e8re et sur divers effets thermiques.<\/p>\n

15. Analyseur de calorim\u00e9trie diff\u00e9rentielle \u00e0 balayage, DSC.<\/p>\n

Principe analytique : l'\u00e9chantillon et la r\u00e9f\u00e9rence se trouvent dans le m\u00eame environnement \u00e0 temp\u00e9rature contr\u00f4l\u00e9e et la variation de l'\u00e9nergie n\u00e9cessaire pour maintenir la diff\u00e9rence de temp\u00e9rature \u00e0 z\u00e9ro est enregistr\u00e9e en fonction de la temp\u00e9rature ambiante ou du temps.<\/p>\n

Repr\u00e9sentation du spectre : courbe de la chaleur ou de son taux de variation en fonction de la temp\u00e9rature ambiante ou du temps.<\/p>\n

Informations fournies : fournir des informations sur la temp\u00e9rature de transition thermique du polym\u00e8re et sur les diff\u00e9rents effets thermiques.<\/p>\n

16. analyseur dynamique de force thermique, DMA.<\/p>\n

Principe de l'analyse : variation de la d\u00e9formation de l'\u00e9chantillon avec la temp\u00e9rature sous l'action d'une force ext\u00e9rieure variant p\u00e9riodiquement.<\/p>\n

Repr\u00e9sentation du spectre : courbe du module ou tan\u03b4 en fonction de la temp\u00e9rature.<\/p>\n

Informations fournies : temp\u00e9rature de transition thermique, module et tan\u03b4.<\/p>","protected":false},"excerpt":{"rendered":"

Spectrom\u00e8tre d'absorption infrarouge, IR Principe analytique : l'absorption de l'\u00e9nergie de la lumi\u00e8re infrarouge provoque des sauts de niveau d'\u00e9nergie de vibration et de rotation des mol\u00e9cules dont les moments dipolaires changent. Repr\u00e9sentation du spectre : variation de l'\u00e9nergie lumineuse transmise relative en fonction de la fr\u00e9quence de la lumi\u00e8re transmise. Informations fournies : emplacement, intensit\u00e9 et forme des pics, [...]<\/p>","protected":false},"author":1,"featured_media":5452,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[106],"tags":[],"class_list":["post-5451","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-laboratory-news"],"yoast_head":"\nIntroduction and principles of common laboratory instrument analysis - Longchang Chemical<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/longchangchemical.com\/fr\/introduction-and-principles-of-common-laboratory-instrument-analysis\/\" \/>\n<meta property=\"og:locale\" content=\"fr_FR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Introduction and principles of common laboratory instrument analysis - Longchang Chemical\" \/>\n<meta property=\"og:description\" content=\"Introduction and principles of common laboratory instrument analysis 1.Infrared absorption spectrometer, IR Analytical principle: absorption of infrared light energy, causing vibrational, rotational energy level jumps of molecules with changing dipole moments. 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