{"id":4495,"date":"2021-04-14T14:26:26","date_gmt":"2021-04-14T14:26:26","guid":{"rendered":"https:\/\/longchangchemical.com\/?p=4495"},"modified":"2024-09-28T11:55:34","modified_gmt":"2024-09-28T11:55:34","slug":"overview-of-enzyme-immobilization-methods","status":"publish","type":"post","link":"https:\/\/longchangchemical.com\/fr\/overview-of-enzyme-immobilization-methods\/","title":{"rendered":"Aper\u00e7u des m\u00e9thodes d'immobilisation des enzymes"},"content":{"rendered":"<h1 class=\"wp-block-heading\"><strong>Aper\u00e7u des m\u00e9thodes d'immobilisation des enzymes<\/strong><\/h1>\r\n<p>Dans l'article pr\u00e9c\u00e9dent, nous avons mentionn\u00e9 que les substances am\u00e8res dans les agrumes sont principalement la naringine, qui peut \u00eatre progressivement d\u00e9compos\u00e9e en glucose et naring\u00e9nine par la naringinase, et finalement atteindre l'objectif de d\u00e9sam\u00e9risation. Dans le processus de production et d'utilisation actuel, les enzymes immobilis\u00e9es, compar\u00e9es aux enzymes libres, ont non seulement une efficacit\u00e9 catalytique plus \u00e9lev\u00e9e et une meilleure sp\u00e9cificit\u00e9, mais elles am\u00e9liorent \u00e9galement la r\u00e9sistance aux conditions difficiles et prolongent la dur\u00e9e de vie des enzymes ; en m\u00eame temps, les enzymes immobilis\u00e9es sont faciles \u00e0 s\u00e9parer du produit de la r\u00e9action, elles simplifient le processus de production et r\u00e9duisent le co\u00fbt. Voici donc une br\u00e8ve introduction aux m\u00e9thodes d'immobilisation des enzymes courantes.<\/p>\r\n<p>&nbsp;<\/p>\r\n<p>\r\n\r\n\r\n\r\n<\/p>\r\n<p>Une enzyme immobilis\u00e9e est une enzyme fix\u00e9e sur un support donn\u00e9 pour la rendre insoluble dans l'eau, tout en conservant ses propri\u00e9t\u00e9s catalytiques. D\u00e8s 1954, Glubhofer et Schleith ont utilis\u00e9 la m\u00e9thode du diazo pour modifier la r\u00e9sine de polystyr\u00e8ne afin d'immobiliser la pepsine et la carboxypeptidase pour pr\u00e9parer des enzymes immobilis\u00e9es. Puis, en 1969, le scientifique japonais Chi Yanichiro a utilis\u00e9 un acide amin\u00e9 acyl\u00e9 immobilis\u00e9 pour r\u00e9aliser pour la premi\u00e8re fois une production continue d'acide amin\u00e9 L, ce qui a favoris\u00e9 le d\u00e9veloppement rapide de la technologie d'immobilisation dans le domaine de l'ing\u00e9nierie enzymatique. Lorsque les enzymes immobilis\u00e9es sont apparues, il existait de nombreux noms diff\u00e9rents, mais lors de la premi\u00e8re conf\u00e9rence internationale sur le g\u00e9nie enzymatique en 1971, il a \u00e9t\u00e9 formellement propos\u00e9 d'utiliser \"enzymes immobilis\u00e9es\" comme terme appropri\u00e9. La r\u00e9action catalys\u00e9e par l'enzyme se d\u00e9roule dans des conditions douces, avec une efficacit\u00e9 catalytique \u00e9lev\u00e9e et une grande sp\u00e9cificit\u00e9. En m\u00eame temps, l'enzyme est facile \u00e0 d\u00e9grader et respecte l'environnement. L'enzyme immobilis\u00e9e conserve non seulement ces caract\u00e9ristiques de l'enzyme, mais poss\u00e8de \u00e9galement de nombreuses propri\u00e9t\u00e9s que les enzymes libres ont du mal \u00e0 poss\u00e9der, telles que : \u2460 facile \u00e0 s\u00e9parer du syst\u00e8me de r\u00e9action ; \u2461 recyclable ; \u2462 am\u00e9lioration de la stabilit\u00e9 de l'enzyme dans des conditions difficiles ; \u2463 possibilit\u00e9 de r\u00e9aliser une catalyse enzymatique en phase non aqueuse ; \u2464 possibilit\u00e9 de produire un syst\u00e8me multi-enzyme avec une r\u00e9action continue. Comme les enzymes immobilis\u00e9es peuvent avoir une valeur d'application importante dans les domaines de la m\u00e9decine, de l'alimentation, de la protection de l'environnement, etc., elles ont attir\u00e9 l'attention des chercheurs ces derni\u00e8res ann\u00e9es et ont de nombreuses applications dans l'industrie.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p class=\"wp-block-image size-large\"><img decoding=\"async\" class=\"wp-image-4471\" src=\"https:\/\/longchangchemical.com\/wp-content\/uploads\/2021\/03\/image-7.png\" alt=\"\" \/><\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>&nbsp;<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>Les m\u00e9thodes courantes d'immobilisation des enzymes comprennent principalement la m\u00e9thode d'adsorption, la m\u00e9thode d'encastrement, la m\u00e9thode de r\u00e9ticulation et la m\u00e9thode de couplage covalent. Les deux premi\u00e8res appartiennent \u00e0 la m\u00e9thode physique et les deux derni\u00e8res \u00e0 la m\u00e9thode chimique. Le principe de ces m\u00e9thodes d'immobilisation des enzymes est illustr\u00e9 \u00e0 la figure 1.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p class=\"wp-block-image size-large\"><img decoding=\"async\" width=\"275\" height=\"222\" class=\"wp-image-4496\" src=\"https:\/\/longchangchemical.com\/wp-content\/uploads\/2021\/04\/image.png\" alt=\"\" \/><\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>&nbsp;<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>Figure 1 M\u00e9thode d'immobilisation de l'enzyme<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p><strong>1<\/strong><strong>\u00a0M\u00e9thode d'adsorption<\/strong><\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>La m\u00e9thode d'adsorption est une m\u00e9thode permettant de compl\u00e9ter l'immobilisation par l'adsorption en surface du support sur l'enzyme ou par l'interaction des charges positives et n\u00e9gatives entre le support et l'enzyme, y compris l'adsorption physique et l'adsorption d'ions.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p class=\"wp-block-image size-large\"><img decoding=\"async\" class=\"wp-image-4473\" src=\"https:\/\/longchangchemical.com\/wp-content\/uploads\/2021\/03\/image-9.png\" alt=\"\" \/><\/p>\r\n<p>\r\n\r\n<\/p>\r\n<ul class=\"wp-block-list\">\r\n<li>\r\n<p>adsorption physique<\/p>\r\n<\/li>\r\n<\/ul>\r\n<p>\r\n\r\n<\/p>\r\n<p>La m\u00e9thode d'adsorption physique consiste \u00e0 fixer l'enzyme \u00e0 la surface du support en utilisant l'effet d'adsorption du support sur l'enzyme. Cette m\u00e9thode est simple \u00e0 mettre en \u0153uvre, ne n\u00e9cessite pas de modification ni d'activation, et le centre actif de l'enzyme est bien pr\u00e9serv\u00e9, mais la force de liaison est faible, et sa force est principalement la force intermol\u00e9culaire et la tension superficielle. Les adsorbants couramment utilis\u00e9s pour l'immobilisation des enzymes comprennent le charbon actif, le graph\u00e8ne, la terre de diatom\u00e9e et d'autres adsorbants non solubles dans l'eau ayant une forte capacit\u00e9 d'absorption de l'eau. Zhao et al. ont utilis\u00e9 de l'oxyde de graph\u00e8ne r\u00e9duit comme support pour co-immobiliser la glucose oxydase et la glucoamylase afin de pr\u00e9parer une enzyme composite immobilis\u00e9e.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p class=\"wp-block-image size-large\"><img decoding=\"async\" class=\"wp-image-4473\" src=\"https:\/\/longchangchemical.com\/wp-content\/uploads\/2021\/03\/image-9.png\" alt=\"\" \/><\/p>\r\n<p>\r\n\r\n<\/p>\r\n<ul class=\"wp-block-list\">\r\n<li>\r\n<p>adsorption d'ions<\/p>\r\n<\/li>\r\n<\/ul>\r\n<p>\r\n\r\n<\/p>\r\n<p>La m\u00e9thode d'adsorption ionique est une m\u00e9thode d'immobilisation de l'enzyme par l'interaction des charges positives et n\u00e9gatives entre le support et l'enzyme. Les conditions de pr\u00e9paration de cette m\u00e9thode sont douces et l'activit\u00e9 de l'enzyme n'est pas facile \u00e0 perdre, mais la force de liaison est \u00e9galement faible et facile \u00e0 perdre. Mateo et al. ont pr\u00e9par\u00e9 un nouveau type de r\u00e9sine \u00e9changeuse d'ions pour fixer respectivement la lipase, la \u03b2-galactosidase et l'aminoacide oxydase. Dans les conditions de pH 7,0 et 4 \u2103, l'enzyme peut atteindre la saturation d'adsorption en quelques minutes, et le taux de r\u00e9cup\u00e9ration de l'activit\u00e9 enzymatique peut atteindre 100%, ce qui montre un bon effet d'immobilisation.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p class=\"wp-block-image size-large\"><img decoding=\"async\" class=\"wp-image-4473\" src=\"https:\/\/longchangchemical.com\/wp-content\/uploads\/2021\/03\/image-9.png\" alt=\"\" \/><\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p><strong>2 M\u00e9thode d'int\u00e9gration<\/strong><\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>La m\u00e9thode d'incorporation est une m\u00e9thode dans laquelle l'enzyme est incorpor\u00e9e dans un mat\u00e9riau poreux, et comprend la m\u00e9thode d'incorporation dans un gel et la m\u00e9thode d'incorporation dans une membrane semi-perm\u00e9able. La m\u00e9thode d'inclusion dans un gel est une m\u00e9thode de fixation dans laquelle l'enzyme est incorpor\u00e9e dans la structure interne du gel (figure 1B-a). Zhang Shuxiang et d'autres ont utilis\u00e9 de l'alginate de sodium pour incorporer de la laccase fongique afin de traiter des eaux us\u00e9es de fabrication de papier \u00e0 faible concentration. Le taux de r\u00e9cup\u00e9ration maximal de l'activit\u00e9 enzymatique \u00e9tait de 48%, et l'activit\u00e9 enzymatique est rest\u00e9e de 64% apr\u00e8s 8 essais. La m\u00e9thode d'encastrement dans une membrane semi-perm\u00e9able consiste \u00e0 immobiliser les enzymes dans une membrane semi-perm\u00e9able compos\u00e9e de polym\u00e8res \u00e0 haute teneur mol\u00e9culaire (figure 1B-b). Cette m\u00e9thode ne n\u00e9cessite pas non plus de modification chimique et n'affecte pas l'activit\u00e9 de l'enzyme, mais il n'est pas facile de contr\u00f4ler la taille de la membrane. Il est difficile de maintenir l'activit\u00e9 de l'enzyme tout en contr\u00f4lant la libre entr\u00e9e et sortie des r\u00e9actifs et des produits sans fuite de l'enzyme. C'est pourquoi cette m\u00e9thode est g\u00e9n\u00e9ralement combin\u00e9e \u00e0 d'autres m\u00e9thodes dans la pratique. Rilling P et al. ont utilis\u00e9 un support pour immobiliser des mol\u00e9cules de prot\u00e9ines dans une microcapsule, ce qui a permis non seulement d'augmenter la stabilit\u00e9 de l'enzyme immobilis\u00e9e, mais aussi d'am\u00e9liorer ses propri\u00e9t\u00e9s.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p class=\"wp-block-image size-large\"><img decoding=\"async\" class=\"wp-image-4473\" src=\"https:\/\/longchangchemical.com\/wp-content\/uploads\/2021\/03\/image-9.png\" alt=\"\" \/><\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p><strong>3 M\u00e9thode de r\u00e9ticulation<\/strong><\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>La m\u00e9thode de r\u00e9ticulation est une m\u00e9thode dans laquelle les mol\u00e9cules d'enzymes sont r\u00e9ticul\u00e9es les unes aux autres \u00e0 l'aide de r\u00e9actifs bifonctionnels ou multifonctionnels, et les mol\u00e9cules d'enzymes et les r\u00e9actifs forment une liaison covalente et sont fix\u00e9s. L'enzyme immobilis\u00e9e pr\u00e9par\u00e9e par cette m\u00e9thode pr\u00e9sente une bonne stabilit\u00e9, mais certains groupes de l'enzyme tels que les groupes amino, sulfhydryle, imidazolyle, etc. peuvent participer \u00e0 la r\u00e9action de r\u00e9ticulation. Si l'agent de r\u00e9ticulation et le groupe fonctionnel s\u00e9lectionn\u00e9s ne sont pas appropri\u00e9s, la structure du centre actif de l'enzyme peut \u00eatre d\u00e9truite, ce qui entra\u00eene une perte importante de l'activit\u00e9 de l'enzyme, c'est pourquoi cette m\u00e9thode est g\u00e9n\u00e9ralement combin\u00e9e \u00e0 d'autres m\u00e9thodes. Li Xiaojing et d'autres ont d'abord encapsul\u00e9 la pepsine dans une solution d'alginate de sodium, puis l'ont ajout\u00e9e goutte \u00e0 goutte \u00e0 une solution contenant une certaine concentration de chitosane et de CaCl<sub>2<\/sub>\u00a0pour obtenir des microsph\u00e8res d'enzymes immobilis\u00e9es, puis ajouter une certaine quantit\u00e9 de glutarald\u00e9hyde pour la r\u00e9ticulation afin d'obtenir de la pepsine immobilis\u00e9e. Cette m\u00e9thode a permis d'obtenir une enzyme immobilis\u00e9e pr\u00e9sentant \u00e0 la fois une bonne stabilit\u00e9 op\u00e9rationnelle et une bonne stabilit\u00e9 thermique.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p class=\"wp-block-image size-large\"><img decoding=\"async\" class=\"wp-image-4473\" src=\"https:\/\/longchangchemical.com\/wp-content\/uploads\/2021\/03\/image-9.png\" alt=\"\" \/><\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p><strong>4 M\u00e9thode de couplage covalent<\/strong><\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>La m\u00e9thode de couplage covalent (\u00e9galement connue sous le nom de m\u00e9thode de liaison covalente) est une m\u00e9thode d'immobilisation de l'enzyme par la formation d'une liaison covalente entre l'enzyme et le support (comme le montre la figure 2).<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p class=\"wp-block-image size-large\"><img decoding=\"async\" width=\"213\" height=\"94\" class=\"wp-image-4497\" src=\"https:\/\/longchangchemical.com\/wp-content\/uploads\/2021\/04\/image-1.png\" alt=\"\" \/><\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>Figure 2 Enzyme immobilis\u00e9e par la m\u00e9thode de liaison covalente, A-r\u00e9sidu d'acide amin\u00e9 actif ; B-groupe de liaison sur le support ; C-porteur.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p class=\"wp-block-image size-large\"><img decoding=\"async\" class=\"wp-image-4473\" src=\"https:\/\/longchangchemical.com\/wp-content\/uploads\/2021\/03\/image-9.png\" alt=\"\" \/><\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>Les m\u00e9thodes de liaison covalente comprennent g\u00e9n\u00e9ralement trois types : (1) le support est modifi\u00e9 et activ\u00e9, puis li\u00e9 de mani\u00e8re covalente \u00e0 la mol\u00e9cule d'enzyme ; (2) le support et la mol\u00e9cule d'enzyme sont li\u00e9s de mani\u00e8re covalente par l'interm\u00e9diaire d'un agent de couplage ; (3) la mol\u00e9cule d'enzyme est modifi\u00e9e apr\u00e8s activation, puis combin\u00e9e avec le support. Le premier type est le plus couramment utilis\u00e9. Apr\u00e8s avoir activ\u00e9 le support pour relier certains groupes actifs tels que les groupes amino, \u00e9poxy, etc., au r\u00e9sidu actif de la mol\u00e9cule d'enzyme (tel que l'amino, le carboxyle, l'hydroxyle, etc.), l'enzyme est immobilis\u00e9e. Cette m\u00e9thode est beaucoup plus fiable que la m\u00e9thode d'adsorption. En m\u00eame temps, comme le support est g\u00e9n\u00e9ralement plus grand, les r\u00e9sidus d'acides amin\u00e9s impliqu\u00e9s dans la r\u00e9action sur l'enzyme sont g\u00e9n\u00e9ralement ceux qui sont expos\u00e9s \u00e0 la p\u00e9riph\u00e9rie de l'enzyme, et l'effet sur le centre actif de l'enzyme est relativement faible. Il s'agit d'une excellente m\u00e9thode d'immobilisation des enzymes. Yan Keliang et d'autres ont utilis\u00e9 des nanoparticules magn\u00e9tiques aminosilanis\u00e9es pour immobiliser la pectinase. Le taux d'immobilisation et le taux de r\u00e9cup\u00e9ration de l'activit\u00e9 enzymatique \u00e9taient respectivement de 44,44% et de 40,86%, ce qui a permis d'obtenir un bon effet d'immobilisation. Le prochain article continuera \u00e0 pr\u00e9senter en d\u00e9tail les diff\u00e9rentes m\u00e9thodes de pr\u00e9paration des microsph\u00e8res magn\u00e9tiques en biopolym\u00e8re.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p class=\"wp-block-image size-large\"><img decoding=\"async\" class=\"wp-image-4473\" src=\"https:\/\/longchangchemical.com\/wp-content\/uploads\/2021\/03\/image-9.png\" alt=\"\" \/><\/p>\r\n<h2><strong><b>Contactez-nous d\u00e8s maintenant !<\/b><\/strong><\/h2>\r\n<h4><strong><b>Si vous avez besoin de Price, veuillez indiquer vos coordonn\u00e9es dans le formulaire ci-dessous. Nous vous contacterons g\u00e9n\u00e9ralement dans les 24 heures. Vous pouvez \u00e9galement m'envoyer un courriel\u00a0<span style=\"color: #00ccff;\"><a style=\"color: #00ccff;\" href=\"mailto:info@longchangchemical.com\">info@longchangchemical.com<\/a><\/span>\u00a0pendant les heures de travail ( 8:30 am to 6:00 pm UTC+8 Mon.~Sat. ) ou utilisez le chat en direct du site web pour obtenir une r\u00e9ponse rapide.<\/b><\/strong><\/h4>\r\n<table style=\"border-collapse: collapse; width: 326.27pt;\" border=\"0\" width=\"435\" cellspacing=\"0\" cellpadding=\"0\">\r\n<tbody>\r\n<tr style=\"height: 13.50pt;\">\r\n<td class=\"et2\" style=\"height: 13.50pt; width: 164.25pt;\" width=\"219\" height=\"18\"><a href=\"https:\/\/longchangchemical.com\/fr\/product\/compound-glucoamylase-cas-9032-08-0\/\"><span style=\"color: #00ccff;\">Compos\u00e9 Glucoamylase<\/span><\/a><\/td>\r\n<td class=\"et2\" style=\"width: 162.00pt;\" width=\"216\">9032-08-0<\/td>\r\n<\/tr>\r\n<tr style=\"height: 13.50pt;\">\r\n<td class=\"et2\" style=\"height: 13.50pt;\" height=\"18\"><a href=\"https:\/\/longchangchemical.com\/fr\/product\/pullulanase-cas-9075-68-7\/\"><span style=\"color: #00ccff;\">Pullulanase<\/span><\/a><\/td>\r\n<td class=\"et2\">9075-68-7<\/td>\r\n<\/tr>\r\n<tr style=\"height: 13.50pt;\">\r\n<td class=\"et2\" style=\"height: 13.50pt;\" height=\"18\"><a href=\"https:\/\/longchangchemical.com\/fr\/product\/xylanase-cas-37278-89-0\/\"><span style=\"color: #00ccff;\">Xylanase<\/span><\/a><\/td>\r\n<td class=\"et2\">37278-89-0<\/td>\r\n<\/tr>\r\n<tr style=\"height: 13.50pt;\">\r\n<td class=\"et2\" style=\"height: 13.50pt;\" height=\"18\"><a href=\"https:\/\/longchangchemical.com\/fr\/product\/cellulase-cas-9012-54-8\/\"><span style=\"color: #00ccff;\">Cellulase<\/span><\/a><\/td>\r\n<td class=\"et2\">9012-54-8<\/td>\r\n<\/tr>\r\n<tr style=\"height: 13.50pt;\">\r\n<td class=\"et2\" style=\"height: 13.50pt;\" height=\"18\"><a href=\"https:\/\/longchangchemical.com\/fr\/product\/naringinase-cas-9068-31-9\/\"><span style=\"color: #00ccff;\">Naringinase<\/span><\/a><\/td>\r\n<td class=\"et2\">9068-31-9<\/td>\r\n<\/tr>\r\n<tr style=\"height: 13.50pt;\">\r\n<td class=\"et2\" style=\"height: 13.50pt;\" height=\"18\"><a href=\"https:\/\/longchangchemical.com\/fr\/product\/beta-amylase-cas-9000-91-3\/\"><span style=\"color: #00ccff;\">\u03b2-Amylase<\/span><\/a><\/td>\r\n<td class=\"et2\">9000-91-3<\/td>\r\n<\/tr>\r\n<tr style=\"height: 13.50pt;\">\r\n<td class=\"et2\" style=\"height: 13.50pt;\" height=\"18\"><a href=\"https:\/\/longchangchemical.com\/fr\/product\/glucose-oxidase-cas-9001-37-0\/\"><span style=\"color: #00ccff;\">Glucose oxydase<\/span><\/a><\/td>\r\n<td class=\"et2\">9001-37-0<\/td>\r\n<\/tr>\r\n<tr style=\"height: 13.50pt;\">\r\n<td class=\"et2\" style=\"height: 13.50pt;\" height=\"18\">alpha-amylase<\/td>\r\n<td class=\"et2\">9000-90-2<\/td>\r\n<\/tr>\r\n<tr style=\"height: 13.50pt;\">\r\n<td class=\"et2\" style=\"height: 13.50pt;\" height=\"18\"><a href=\"https:\/\/longchangchemical.com\/fr\/product\/longzyme-acid-pectinase-cas-9032-75-1\/\"><span style=\"color: #00ccff;\">Pectinase<\/span><\/a><\/td>\r\n<td class=\"et2\">9032-75-1<\/td>\r\n<\/tr>\r\n<tr style=\"height: 13.50pt;\">\r\n<td class=\"et2\" style=\"height: 13.50pt;\" height=\"18\">Peroxydase<\/td>\r\n<td class=\"et2\">9003-99-0<\/td>\r\n<\/tr>\r\n<tr style=\"height: 13.50pt;\">\r\n<td class=\"et2\" style=\"height: 13.50pt;\" height=\"18\"><a href=\"https:\/\/longchangchemical.com\/fr\/product\/lipase-cas-9001-62-1\/\"><span style=\"color: #00ccff;\">Lipase<\/span><\/a><\/td>\r\n<td class=\"et2\">9001-62-1<\/td>\r\n<\/tr>\r\n<tr style=\"height: 13.50pt;\">\r\n<td class=\"et2\" style=\"height: 13.50pt;\" height=\"18\"><a href=\"https:\/\/longchangchemical.com\/fr\/product\/catalase-cas-9001-05-2\/\"><span style=\"color: #00ccff;\">Catalase<\/span><\/a><\/td>\r\n<td class=\"et4\">9001-05-2<\/td>\r\n<\/tr>\r\n<tr style=\"height: 13.50pt;\">\r\n<td class=\"et2\" style=\"height: 13.50pt;\" height=\"18\"><a href=\"https:\/\/longchangchemical.com\/fr\/product\/tannase-cas-9025-71-2\/\"><span style=\"color: #00ccff;\">TANNASE<\/span><\/a><\/td>\r\n<td class=\"et2\">9025-71-2<\/td>\r\n<\/tr>\r\n<tr style=\"height: 13.50pt;\">\r\n<td class=\"et2\" style=\"height: 13.50pt;\" height=\"18\"><a href=\"https:\/\/longchangchemical.com\/fr\/product\/elastase-cas-39445-21-1\/\"><span style=\"color: #00ccff;\">Elastase<\/span><\/a><\/td>\r\n<td class=\"et2\">39445-21-1<\/td>\r\n<\/tr>\r\n<tr style=\"height: 13.50pt;\">\r\n<td class=\"et2\" style=\"height: 13.50pt;\" height=\"18\"><a href=\"https:\/\/longchangchemical.com\/fr\/product\/urease-cas-9002-13-5\/\"><span style=\"color: #00ccff;\">Ur\u00e9ase<\/span><\/a><\/td>\r\n<td class=\"et2\">9002-13-5<\/td>\r\n<\/tr>\r\n<tr style=\"height: 13.50pt;\">\r\n<td class=\"et2\" style=\"height: 13.50pt;\" height=\"18\"><a href=\"https:\/\/longchangchemical.com\/fr\/product\/dextranase-cas-9025-70-1\/\"><span style=\"color: #00ccff;\">DEXTRANASE<\/span><\/a><\/td>\r\n<td class=\"et2\">9025-70-1<\/td>\r\n<\/tr>\r\n<tr style=\"height: 13.50pt;\">\r\n<td class=\"et2\" style=\"height: 13.5pt; text-align: left;\" height=\"18\"><a href=\"https:\/\/longchangchemical.com\/fr\/product\/l-lactic-dehydrogenase-cas-9001-60-9\/\"><span style=\"color: #00ccff;\">L-Lactique d\u00e9shydrog\u00e9nase<\/span><\/a><\/td>\r\n<td class=\"et2\">9001-60-9<\/td>\r\n<\/tr>\r\n<tr style=\"height: 13.50pt;\">\r\n<td class=\"et2\" style=\"height: 13.50pt;\" height=\"18\"><a href=\"https:\/\/longchangchemical.com\/fr\/product\/dehydrogenase-malate-cas-9001-64-3\/\"><span style=\"color: #00ccff;\">D\u00e9shydrog\u00e9nase malate<\/span><\/a><\/td>\r\n<td class=\"et2\">9001-64-3<\/td>\r\n<\/tr>\r\n<tr style=\"height: 13.50pt;\">\r\n<td class=\"et2\" style=\"height: 13.50pt;\" height=\"18\"><a href=\"https:\/\/longchangchemical.com\/fr\/product\/cholesterol-oxidase-cas-9028-76-6\/\"><span style=\"color: #00ccff;\">Cholest\u00e9rol oxydase<\/span><\/a><\/td>\r\n<td class=\"et2\">9028-76-6<\/td>\r\n<\/tr>\r\n<\/tbody>\r\n<\/table>","protected":false},"excerpt":{"rendered":"<p>Aper\u00e7u des m\u00e9thodes d'immobilisation des enzymes Dans l'article pr\u00e9c\u00e9dent, nous avons mentionn\u00e9 que les substances am\u00e8res des agrumes sont principalement constitu\u00e9es de naringine, qui peut \u00eatre progressivement d\u00e9compos\u00e9e en glucose et en naring\u00e9nine par la naringinase, et finalement atteindre l'objectif de l'amertume. Dans le processus de production et d'utilisation actuel, par rapport aux enzymes libres, les enzymes immobilis\u00e9es ont non seulement [...]<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[108],"tags":[],"class_list":["post-4495","post","type-post","status-publish","format-standard","hentry","category-enzyme-news"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v25.3.1 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Overview of enzyme immobilization methods - Longchang Chemical<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/longchangchemical.com\/fr\/overview-of-enzyme-immobilization-methods\/\" \/>\n<meta property=\"og:locale\" content=\"fr_FR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Overview of enzyme immobilization methods - Longchang Chemical\" \/>\n<meta property=\"og:description\" content=\"Overview of enzyme immobilization methods In the previous article, we mentioned that the bitter substances in citrus fruits are mainly naringin, which can be gradually decomposed into glucose and naringenin by naringinase, and finally achieve the purpose of debittering. 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