{"id":4429,"date":"2021-03-09T14:01:53","date_gmt":"2021-03-09T14:01:53","guid":{"rendered":"https:\/\/longchangchemical.com\/?p=4429"},"modified":"2024-09-28T11:54:19","modified_gmt":"2024-09-28T11:54:19","slug":"biotransformation-of-saponins","status":"publish","type":"post","link":"https:\/\/longchangchemical.com\/fr\/biotransformation-of-saponins\/","title":{"rendered":"Biotransformation des saponines"},"content":{"rendered":"

Les saponines sont une classe de glycosides dont les aglycones sont des compos\u00e9s triterp\u00e9niques ou st\u00e9riques. Elles constituent l'un des ingr\u00e9dients efficaces de nombreuses plantes m\u00e9dicinales chinoises telles que le ginseng, la r\u00e9glisse et l'igname (la structure principale des saponines est pr\u00e9sent\u00e9e \u00e0 la figure 1). Renforcer l'immunit\u00e9 et d'autres fonctions. Il existe de nombreux rapports sur la biotransformation des gins\u00e9nosides dans la litt\u00e9rature. \u00c0 l'heure actuelle, plus de 150 types de gins\u00e9nosides ont \u00e9t\u00e9 s\u00e9par\u00e9s et identifi\u00e9s. Les teneurs en gins\u00e9nosides Rb1, Rb2, Rc, Rd, Re et Rg1 atteignent 80%, tandis que les teneurs en gins\u00e9nosides Rg3, Rh2, F2, compos\u00e9 K (C-K) et autres saponines rares sont faibles ou inexistantes. Des \u00e9tudes ont montr\u00e9 que certaines saponines rares ont de bonnes activit\u00e9s pharmacologiques. Toutefois, en raison de leur faible teneur, leur pr\u00e9paration et leur production sont limit\u00e9es. Le m\u00eame type de gins\u00e9noside poss\u00e8de le m\u00eame aglycone, mais la cha\u00eene de sucre est diff\u00e9rente. Les gins\u00e9nosides rares et la teneur plus \u00e9lev\u00e9e du m\u00eame type de saponines ne diff\u00e8rent souvent que par 2 ou 3 groupes de sucres. Par cons\u00e9quent, le m\u00eame type de saponine rare active peut \u00eatre pr\u00e9par\u00e9 par hydrolyse enzymatique de la saponine \u00e0 haute teneur.<\/h4>\r\n\r\n\r\n\r\n
\"\"<\/figure>\r\n\r\n\r\n\r\n

Figure 1. Structure des principales saponines<\/h4>\r\n\r\n\r\n\r\n

Les diff\u00e9rentes glycosides hydrolases ont des s\u00e9lectivit\u00e9s diff\u00e9rentes et les voies d'hydrolyse des gins\u00e9nosides sont \u00e9galement diff\u00e9rentes. Comme le montre le tableau 1, diff\u00e9rentes glycosides hydrolases peuvent \u00eatre utilis\u00e9es pour pr\u00e9parer diff\u00e9rents gins\u00e9nosides rares. Le gins\u00e9noside Rd peut \u00eatre pr\u00e9par\u00e9 en hydrolysant les gins\u00e9nosides Rb1, Rb2, Rb3 et le groupe de sucre externe C-20 de Rc. La \u03b2-glucosidase isol\u00e9e et purifi\u00e9e de l'escargot de jade blanc de Chine et de Thermus caldophilus peut convertir le gins\u00e9noside Rb1 en Rd. Kim et al. l'ont obtenue \u00e0 partir de micro-organismes du sol en utilisant la technologie du clonage mol\u00e9culaire pour convertir le gins\u00e9noside Rb1, qui est la glycoside hydrolase recombinante du Rd. Par la suite, les chercheurs ont clon\u00e9 la glucosidase de Thermotoga thermarum et de Bifidobacterium longum H-1, ce qui a am\u00e9lior\u00e9 l'efficacit\u00e9 de la transformation et de la pr\u00e9paration du gins\u00e9noside Rd. La glucosidase obtenue \u00e0 partir de Flavobacterium johnsoniae et Thermus thermophilus par technologie recombinante peut non seulement convertir le gins\u00e9noside Rb1 en Rd, mais aussi hydrolyser la cha\u00eene de sucre C-20 du gyp\u00e9noside XVII (G17) pour produire le gins\u00e9noside F2. Outre la glucosidase, l'\u03b1-L-arabinofuranoside hydrolase qui peut convertir le gins\u00e9noside Rc en Rd a \u00e9t\u00e9 obtenue \u00e0 partir de racines de ginseng et de Leuconostoc sp. La \u0391-L-arabinofuranoside hydrolase et l'\u03b1-L-arabinopyranoside hydrolase sont obtenues \u00e0 partir de Bifidobacterium breve et Bifidobacterium longum, qui peuvent transformer le gins\u00e9noside Rc et Rb2 en Rd. Il a \u00e9t\u00e9 rapport\u00e9 dans la litt\u00e9rature que l'\u03b1-L-arabinofuranoside hydrolase de Caldicellulosiruptor saccharolyticus et Rhodanobacter ginsenosidimutans peut non seulement hydrolyser le gins\u00e9noside Rc en Rd, mais aussi convertir le compos\u00e9 Mc1 (C-Mc1) en F2. La glycoside hydrolase isol\u00e9e et purifi\u00e9e \u00e0 partir d'Aspergillus par Yu et al. peut convertir tous les gins\u00e9nosides Rb1, Rb2, Rb3 et Rc en Rd. Certaines glycosides hydrolases peuvent hydrolyser compl\u00e8tement les cha\u00eenes de sucre en position C-20 dans des mol\u00e9cules telles que les gins\u00e9nosides de type glycol Rb1, Rb2, Rb3, Rc et Rd, pour g\u00e9n\u00e9rer le gins\u00e9noside Rg3, qui permet une production \u00e0 grande \u00e9chelle de Rg3 et est d\u00e9velopp\u00e9 en tant que m\u00e9dicament anti-tumoral. La glucosidase de Paecilomyces bainier et de Microbacterium esteraromaticum peut hydrolyser directement le gins\u00e9noside Rb1 en Rg3, tandis que la glucosidase isol\u00e9e et purifi\u00e9e de Microbacterium esteraromaticum peut hydrolyser le gins\u00e9noside Rb2 en Rg3. La glycoside hydrolase recombinante clon\u00e9e \u00e0 partir de Pseudonocardia par la technologie du clonage mol\u00e9culaire peut transformer les gins\u00e9nosides Rb1, Rb3 et Rd pour pr\u00e9parer le Rg3. De m\u00eame, une s\u00e9rie de gins\u00e9nosides rares et actifs peut \u00eatre pr\u00e9par\u00e9e en hydrolysant le groupe de sucre en position C-3 dans les gins\u00e9nosides. La glucosidase recombinante clon\u00e9e \u00e0 partir de Sphingomonas et de Sphingopyxis alaskensis peut hydrolyser le glucose \u00e0 l'ext\u00e9rieur de la cha\u00eene de sucre en position C-3 dans les mol\u00e9cules de gins\u00e9noside Rb1, Rb2, Rc, Rd et Rg3, et pr\u00e9parer le G17, le compos\u00e9 O (CO) et les C-Mc1, F2 et Rh2. Certaines glycosidases peuvent hydrolyser directement le groupe glucosyle interne en position C-3. Par exemple, la glucosidase de Terrabacter ginsenosidimutans et Esteya vermicola peut hydrolyser la cha\u00eene de sucre en position C-3 des mol\u00e9cules de gins\u00e9noside Rb1, Rb2, Rb3, Rc et Rd pour produire la saponine correspondante LXXV (G75), le compos\u00e9 Y (C-Y), le compos\u00e9 Mx (C-Mx), le compos\u00e9 Mc (C-Mc) et C-K. En outre, certaines glycosides hydrolases peuvent hydrolyser simultan\u00e9ment les groupes de sucre C-20 et C-3 dans les gins\u00e9nosides de type glycol. La glucosidase recombinante clon\u00e9e \u00e0 partir de l'Arthrobacter chlorophenolicus peut convertir les gins\u00e9nosides Rb1, Rb2 et Rc en F2. La glycoside hydrolase de Fusobacterium K60, des champignons endophytes GE 17-18, de Sulfolobus acidocaldarius, d'Aspergillus niger et de Microbacteriu esteraromaticum peut hydrolyser le gins\u00e9noside Rb1 pour produire C-K.<\/h4>\r\n\r\n\r\n\r\n

Tableau 1. Biotransformation des gins\u00e9nosides par les glycosidases<\/h4>\r\n\r\n\r\n\r\n
\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n
Produit<\/td>\r\nSubstrat<\/td>\r\nR\u00e9action<\/td>\r\nOrganisme<\/td>\r\n<\/tr>\r\n
Rd<\/td>\r\nRb1<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nEscargot de jade blanc de Chine<\/td>\r\n<\/tr>\r\n
Rd<\/td>\r\nRb1<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nThermus<\/em>\u00a0<\/em>caldophile<\/em><\/td>\r\n<\/tr>\r\n
Rd<\/td>\r\nRb1<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nBact\u00e9ries non cultiv\u00e9es<\/td>\r\n<\/tr>\r\n
Rd<\/td>\r\nRb1<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nThermotoga<\/em>\u00a0<\/em>thermarum<\/em><\/td>\r\n<\/tr>\r\n
Rd<\/td>\r\nRb1<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nBifidobacterium<\/em>\u00a0<\/em>longum<\/em>\u00a0<\/em>H-1<\/td>\r\n<\/tr>\r\n
Rd<\/td>\r\nRb1<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nFlavobacterium<\/em>\u00a0<\/em>johnsoniae<\/em><\/td>\r\n<\/tr>\r\n
Rd<\/td>\r\nRb1<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nThermus<\/em>\u00a0<\/em>thermophilus<\/em><\/td>\r\n<\/tr>\r\n
Rd<\/td>\r\nRb1<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nPenicillium<\/em>\u00a0<\/em>oxalicum<\/em><\/td>\r\n<\/tr>\r\n
Rd<\/td>\r\nRb1<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nCladosporium<\/em>\u00a0<\/em>fulvum<\/em><\/td>\r\n<\/tr>\r\n
Rd<\/td>\r\nRc<\/td>\r\n\u03b1-L-Arabinofuranosidase<\/td>\r\nPanax<\/em>\u00a0<\/em>ginseng<\/em><\/td>\r\n<\/tr>\r\n
Rd<\/td>\r\nRc<\/td>\r\n\u03b1-L-Arabinofuranosidase<\/td>\r\nLeuconostoc<\/em><\/td>\r\n<\/tr>\r\n
Rd<\/td>\r\nRc<\/td>\r\n\u03b1-L-Arabinofuranosidase<\/td>\r\nBifidobacterium<\/em>\u00a0<\/em>bref<\/em><\/td>\r\n<\/tr>\r\n
Rd<\/td>\r\nRc<\/td>\r\n\u03b1-L-Arabinofuranosidase<\/td>\r\nBifidobacterium<\/em>\u00a0<\/em>longum<\/em><\/td>\r\n<\/tr>\r\n
Rd<\/td>\r\nRc<\/td>\r\n\u03b1-L-Arabinofuranosidase<\/td>\r\nCaldicellulosiruptor<\/em>\u00a0<\/em>saccharolyticus<\/em><\/td>\r\n<\/tr>\r\n
Rd<\/td>\r\nRc<\/td>\r\n\u03b1-L-Arabinofuranosidase<\/td>\r\nRhodanobacter<\/em>\u00a0<\/em>ginsenosidimutans<\/em><\/td>\r\n<\/tr>\r\n
Rd<\/td>\r\nRb2<\/td>\r\n\u03b1-L-Arabinopyranosidase<\/td>\r\nBifidobacterium<\/em>\u00a0<\/em>bref<\/em><\/td>\r\n<\/tr>\r\n
Rd<\/td>\r\nRb2<\/td>\r\n\u03b1-L-Arabinopyranosidase<\/td>\r\nBifidobacterium<\/em>\u00a0<\/em>longum<\/em><\/td>\r\n<\/tr>\r\n
Rd<\/td>\r\nRb1\/Rb2\/Rb3\/Rc<\/td>\r\nGlycosidase<\/td>\r\nAspergillus<\/em><\/td>\r\n<\/tr>\r\n
Rg3<\/td>\r\nRb1<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nPaecilomyces<\/em>\u00a0<\/em>bainier<\/em><\/td>\r\n<\/tr>\r\n
Rg3<\/td>\r\nRb1<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nMicrobact\u00e9ries<\/em>\u00a0<\/em>esteraromaticum<\/em><\/td>\r\n<\/tr>\r\n
Rg3<\/td>\r\nRb2<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nMicrobact\u00e9ries<\/em>\u00a0<\/em>esteraromaticum<\/em><\/td>\r\n<\/tr>\r\n
Rg3<\/td>\r\nRb1\/Rb3\/Rd<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nPseudonocardia<\/em><\/td>\r\n<\/tr>\r\n
G17<\/td>\r\nRb1<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nSphingomonas<\/em><\/td>\r\n<\/tr>\r\n
G17<\/td>\r\nRb1<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nSphingopyxis<\/em>\u00a0<\/em>alaskensis<\/em><\/td>\r\n<\/tr>\r\n
G17<\/td>\r\nRb1<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nCellulosimicrobium<\/em>\u00a0<\/em>celluloses<\/em><\/td>\r\n<\/tr>\r\n
G75<\/td>\r\nRb1<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nTerrabacter<\/em>\u00a0<\/em>ginsenosidimutans<\/em><\/td>\r\n<\/tr>\r\n
G75<\/td>\r\nRb1<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nEsteya<\/em>\u00a0<\/em>vermicola<\/em><\/td>\r\n<\/tr>\r\n
F2<\/td>\r\nG17<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nFlavobacterium<\/em>\u00a0<\/em>johnsoniae<\/em><\/td>\r\n<\/tr>\r\n
F2<\/td>\r\nG17<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nThermus<\/em>\u00a0<\/em>thermophilus<\/em><\/td>\r\n<\/tr>\r\n
F2<\/td>\r\nC-Mc1<\/sub><\/td>\r\n\u03b1-L-Arabinofuranosidase<\/td>\r\nCaldicellulosiruptor<\/em>\u00a0<\/em>saccharolyticus<\/em><\/td>\r\n<\/tr>\r\n
F2<\/td>\r\nC-Mc1<\/sub><\/td>\r\n\u03b1-L-Arabinofuranosidase<\/td>\r\nRhodanobacter<\/em>\u00a0<\/em>ginsenosidimutans<\/em><\/td>\r\n<\/tr>\r\n
F2<\/td>\r\nRd<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nCellulosimicrobium<\/em>\u00a0<\/em>celluloses<\/em><\/td>\r\n<\/tr>\r\n
F2<\/td>\r\nRb1\/Rb2\/Rc<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nArthrobacter<\/em>\u00a0<\/em>chloroph\u00e9nolique<\/em><\/td>\r\n<\/tr>\r\n
Rh2<\/td>\r\nRg3<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nSphingopyxis<\/em>\u00a0<\/em>alaskensis<\/em><\/td>\r\n<\/tr>\r\n
CK<\/td>\r\nRd<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nTerrabacter<\/em>\u00a0<\/em>ginsenosidimutans<\/em><\/td>\r\n<\/tr>\r\n
CK<\/td>\r\nRd<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nEsteya<\/em>\u00a0<\/em>vermicola<\/em><\/td>\r\n<\/tr>\r\n
CK<\/td>\r\nRb1<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nFusobacterium<\/em>\u00a0<\/em>K-60<\/td>\r\n<\/tr>\r\n
CK<\/td>\r\nRb1<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nchampignons endophytes GE 17-18<\/td>\r\n<\/tr>\r\n
CK<\/td>\r\nRb1\/Rb2<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nSulfolobus<\/em>\u00a0<\/em>acidocaldarius<\/em><\/td>\r\n<\/tr>\r\n
CK<\/td>\r\nRb1\/Rb2\/Rb3\/Rc<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nAspergillus<\/em>\u00a0<\/em>niger<\/em><\/td>\r\n<\/tr>\r\n
CK<\/td>\r\nRb1\/Rb2<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nMicrobacteriu<\/em>\u00a0<\/em>esteraromaticum<\/em><\/td>\r\n<\/tr>\r\n
C-O<\/td>\r\nRb2<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nCellulosimicrobium<\/em>\u00a0<\/em>celluloses<\/em><\/td>\r\n<\/tr>\r\n
C-Y<\/td>\r\nRb2<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nTerrabacter ginsenosidimutans<\/em><\/td>\r\n<\/tr>\r\n
C-Mc<\/td>\r\nRc<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nTerrabacter ginsenosidimutans<\/em><\/td>\r\n<\/tr>\r\n
C-Mc1<\/td>\r\nRc<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nCellulosimicrobium cellulans<\/em><\/td>\r\n<\/tr>\r\n
C-Mx<\/td>\r\nRb3<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nTerrabacter ginsenosidimutans<\/em><\/td>\r\n<\/tr>\r\n
Rg2<\/td>\r\nRe<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nMicrobacterium esteraromaticum<\/em><\/td>\r\n<\/tr>\r\n
Rg2<\/td>\r\nRe<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nMucilaginibacter<\/em><\/td>\r\n<\/tr>\r\n
Rg2<\/td>\r\nRe<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nPseudonocardia<\/em><\/td>\r\n<\/tr>\r\n
Rh1<\/td>\r\nRg1<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nMicrobacterium esteraromaticum<\/em><\/td>\r\n<\/tr>\r\n
Rh1<\/td>\r\nRf<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nPyrococcus furiosus<\/em><\/td>\r\n<\/tr>\r\n
Rh1<\/td>\r\nRf<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nAspergillus niger<\/em><\/td>\r\n<\/tr>\r\n
Rh1<\/td>\r\nRg2<\/td>\r\n\u03b1-L-Rhamnosidase<\/td>\r\nAbsidia<\/em><\/td>\r\n<\/tr>\r\n
Rh1<\/td>\r\nR2<\/td>\r\n\u03b2-Xylosidase<\/td>\r\nThermoanaerobacterium<\/em><\/td>\r\n<\/tr>\r\n
F1<\/td>\r\nRg1<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nFusarium moniliforme<\/em><\/td>\r\n<\/tr>\r\n
F1<\/td>\r\nRg1<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nPenicillium sclerotiorum<\/em><\/td>\r\n<\/tr>\r\n
F1<\/td>\r\nRg1<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nSanguibacter keddieii<\/em><\/td>\r\n<\/tr>\r\n<\/tbody>\r\n<\/table>\r\n<\/figure>\r\n\r\n\r\n\r\n

G17 : gyp\u00e9noside XVII ; G75 : gyp\u00e9noside LXXV ; C-O : compos\u00e9 O ; C-Y : compos\u00e9 Y ; C-Mc1 : compos\u00e9 Mc1 ; C-Mc : compos\u00e9 Mc ; C-Mx : compos\u00e9 Mx ; C-K : compos\u00e9 K.<\/h4>\r\n\r\n\r\n\r\n

Les groupes de sucres C-6 et C-20 des gins\u00e9nosides triols peuvent \u00e9galement \u00eatre hydrolys\u00e9s par les glycosides hydrolases. Le gins\u00e9noside Rg2 peut \u00eatre obtenu en hydrolysant le glucose C-20 dans la mol\u00e9cule Re par la glycosidase. La glucosidase recombinante clon\u00e9e \u00e0 partir de Microbacterium esteraromaticum, Mucillaginibacter et Pseudonocardia peut non seulement convertir le gins\u00e9noside Re en Rg2, mais aussi le gins\u00e9noside Rg1 en Rh1. Le glucose, le rhamnose et le xylose en dehors de la position C-6 des gins\u00e9nosides Rf, Rg2 et R2 peuvent tous \u00eatre convertis pour pr\u00e9parer le Rh1. Contrairement au gins\u00e9noside Rh1, le gins\u00e9noside F1 n'a qu'un seul glucose attach\u00e9 \u00e0 la position C-20 de son aglycone. La glucosidase de Fusarium moniliforme, Penicillium sclerotiorum et Sanguibacter keddieii peut hydrolyser sp\u00e9cifiquement le glucose C-6 du gins\u00e9noside Rg1 pour produire le gins\u00e9noside F1.<\/h4>\r\n\r\n\r\n\r\n

La glycoside hydrolase n'est pas seulement utilis\u00e9e pour transformer et pr\u00e9parer des gins\u00e9nosides rares et actifs, mais elle est \u00e9galement largement utilis\u00e9e pour hydrolyser et modifier des saponines telles que la r\u00e9glisse, le soja et l'igname (tableau 2). La glucuronidase isol\u00e9e et purifi\u00e9e \u00e0 partir de Streptococcus LJ-22 et Penicillium purpurogenum Li-3 peut hydrolyser la glycyrrhizine pour produire de l'acide monoglucuronique glycyrrhizine, et il n'y a pas de sous-produit d'acide glycyrrh\u00e9tinique. Morana et al. ont utilis\u00e9 la glucuronidase d\u00e9riv\u00e9e d'Aspergillus niger pour hydrolyser compl\u00e8tement la glycyrrhizine et produire de l'acide glycyrrh\u00e9tinique. La soyasaponine hydrolase isol\u00e9e et purifi\u00e9e d'Aspergillus oryzae peut hydrolyser la soyasaponine I pour produire le soyasaponol B. Une nouvelle saponine hydrolase de soja dans Neocosmospora vasinfecta peut convertir les saponines de soja I, II et III en saponine de soja B, ce qui constitue un outil efficace pour pr\u00e9parer la saponine de soja avec un effet anti-oxydant et une r\u00e9gulation des lipides sanguins. Parmi les saponines st\u00e9ro\u00efdiennes, la recherche et la comparaison de la modification par hydrolyse de la cha\u00eene de sucre de la dioscine sont syst\u00e9matiques. Inoue et al. ont isol\u00e9 et purifi\u00e9 une glucosidase de Costus speciosus qui peut hydrolyser la diosg\u00e9nine originale pour produire de la diosg\u00e9nine. Liu et al. ont isol\u00e9, purifi\u00e9 et clon\u00e9 \u00e0 partir d'Aspergillus oryzae pour obtenir une dioscine hydrolase recombinante, qui peut hydrolyser les groupes glucosyl et \u03b1-1,4 rhamnosyl de la dioscine pour produire de la dioscine III. L'\u03b1-L-rhamnosidase isol\u00e9e et purifi\u00e9e par Feng et al. \u00e0 partir de Curvularia lunata peut hydrolyser le groupe \u03b1-1,2 rhamnosyl de la dioscine pour produire la dioscine V. Qian et al. ont isol\u00e9 et purifi\u00e9 une \u03b1-L-rhamnosidase \u00e0 partir de foie de b\u0153uf frais, qui peut hydrolyser les groupes \u03b1-1,2 et \u03b1-1,4 rhamnosyl de la diosg\u00e9nine pour former un groupe glucose. -Diosg\u00e9nine. Fu et al. ont isol\u00e9 et purifi\u00e9 la diosg\u00e9nine hydrolase de l'Absidia, qui peut hydrolyser compl\u00e8tement la diosg\u00e9nine en diosg\u00e9nine.<\/h4>\r\n\r\n\r\n\r\n

Tableau 2. Biotransformation d'autres saponines par la glycosidase<\/h4>\r\n\r\n\r\n\r\n
\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n
Produit<\/td>\r\nSubstrat<\/td>\r\nR\u00e9action<\/td>\r\nOrganisme<\/td>\r\n<\/tr>\r\n
GAMG<\/td>\r\nGlycyrrhizine<\/td>\r\n\u03b2-Glucuronidase<\/td>\r\nStreptocoques<\/em><\/td>\r\n<\/tr>\r\n
GAMG<\/td>\r\nGlycyrrhizine<\/td>\r\n\u03b2-Glucuronidase<\/td>\r\nPenicillium purpurogenum<\/em><\/td>\r\n<\/tr>\r\n
Acide glycyrrh\u00e9tinique<\/td>\r\nGlycyrrhizine<\/td>\r\n\u03b2-Glucuronidase<\/td>\r\nAspergillus niger<\/em><\/td>\r\n<\/tr>\r\n
Soyasapog\u00e9nol B<\/td>\r\nSoyasaponine I<\/td>\r\nSaponine hydrolase de soja<\/td>\r\nAspergillus oryzae<\/em><\/td>\r\n<\/tr>\r\n
Soyasapog\u00e9nol B<\/td>\r\nSoyasaponine<\/td>\r\nSaponine hydrolase de soja<\/td>\r\nNeocosmospora vasinfecta<\/em><\/td>\r\n<\/tr>\r\n
Dioscin<\/td>\r\nProtodioscine<\/td>\r\n\u03b2-Glucosidase<\/td>\r\nCostus speciosus<\/em><\/td>\r\n<\/tr>\r\n
Prog\u00e9nine III<\/td>\r\nProtodioscine<\/td>\r\nProtodioscine-glycosidase<\/td>\r\nAspergillus oryzae<\/em><\/td>\r\n<\/tr>\r\n
Prog\u00e9nine V<\/td>\r\nDioscin<\/td>\r\n\u03b1-L-Rhamnosidase<\/td>\r\nCurvularia lunata<\/em><\/td>\r\n<\/tr>\r\n
Diosg\u00e9nyl-glucoside<\/td>\r\nDioscin<\/td>\r\n\u03b1-L-Rhamnosidase<\/td>\r\nFoie bovin<\/em><\/td>\r\n<\/tr>\r\n
Diosg\u00e9nine<\/td>\r\nDioscin<\/td>\r\nDioscin-glycosidase<\/td>\r\nAbsidia<\/em><\/td>\r\n<\/tr>\r\n<\/tbody>\r\n<\/table>\r\n<\/figure>\r\n\r\n\r\n\r\n

GAMG : Mono-glucuronide d'acide glycyrrh\u00e9tique<\/h4>\r\n

Contactez-nous d\u00e8s maintenant !<\/b><\/strong><\/h2>\r\n

Si vous avez besoin de Price, veuillez indiquer vos coordonn\u00e9es dans le formulaire ci-dessous. Nous vous contacterons g\u00e9n\u00e9ralement dans les 24 heures. Vous pouvez \u00e9galement m'envoyer un courriel\u00a0info@longchangchemical.com<\/a><\/span>\u00a0pendant les heures de travail ( 8:30 am to 6:00 pm UTC+8 Mon.~Sat. ) ou utilisez le chat en direct du site web pour obtenir une r\u00e9ponse rapide.<\/b><\/strong><\/h4>\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n
Compos\u00e9 Glucoamylase<\/span><\/a><\/td>\r\n9032-08-0<\/td>\r\n<\/tr>\r\n
Pullulanase<\/span><\/a><\/td>\r\n9075-68-7<\/td>\r\n<\/tr>\r\n
Xylanase<\/span><\/a><\/td>\r\n37278-89-0<\/td>\r\n<\/tr>\r\n
Cellulase<\/span><\/a><\/td>\r\n9012-54-8<\/td>\r\n<\/tr>\r\n
Naringinase<\/span><\/a><\/td>\r\n9068-31-9<\/td>\r\n<\/tr>\r\n
\u03b2-Amylase<\/span><\/a><\/td>\r\n9000-91-3<\/td>\r\n<\/tr>\r\n
Glucose oxydase<\/span><\/a><\/td>\r\n9001-37-0<\/td>\r\n<\/tr>\r\n
alpha-amylase<\/td>\r\n9000-90-2<\/td>\r\n<\/tr>\r\n
Pectinase<\/span><\/a><\/td>\r\n9032-75-1<\/td>\r\n<\/tr>\r\n
Peroxydase<\/td>\r\n9003-99-0<\/td>\r\n<\/tr>\r\n
Lipase<\/span><\/a><\/td>\r\n9001-62-1<\/td>\r\n<\/tr>\r\n
Catalase<\/span><\/a><\/td>\r\n9001-05-2<\/td>\r\n<\/tr>\r\n
TANNASE<\/span><\/a><\/td>\r\n9025-71-2<\/td>\r\n<\/tr>\r\n
Elastase<\/span><\/a><\/td>\r\n39445-21-1<\/td>\r\n<\/tr>\r\n
Ur\u00e9ase<\/span><\/a><\/td>\r\n9002-13-5<\/td>\r\n<\/tr>\r\n
DEXTRANASE<\/span><\/a><\/td>\r\n9025-70-1<\/td>\r\n<\/tr>\r\n
L-Lactique d\u00e9shydrog\u00e9nase<\/span><\/a><\/td>\r\n9001-60-9<\/td>\r\n<\/tr>\r\n
D\u00e9shydrog\u00e9nase malate<\/span><\/a><\/td>\r\n9001-64-3<\/td>\r\n<\/tr>\r\n
Cholest\u00e9rol oxydase<\/span><\/a><\/td>\r\n9028-76-6<\/td>\r\n<\/tr>\r\n<\/tbody>\r\n<\/table>","protected":false},"excerpt":{"rendered":"

Les saponines sont une classe de glycosides dont les aglycones sont des compos\u00e9s triterp\u00e9niques ou st\u00e9riques. Elles constituent l'un des ingr\u00e9dients efficaces de nombreuses plantes m\u00e9dicinales chinoises telles que le ginseng, la r\u00e9glisse et l'igname (la structure principale des saponines est pr\u00e9sent\u00e9e \u00e0 la figure 1). Renforcer l'immunit\u00e9 et d'autres fonctions. Il existe de nombreux rapports sur la biotransformation des gins\u00e9nosides [...]<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[108],"tags":[],"class_list":["post-4429","post","type-post","status-publish","format-standard","hentry","category-enzyme-news"],"yoast_head":"\nBiotransformation of saponins - Longchang Chemical Co., Ltd.<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/longchangchemical.com\/fr\/biotransformation-of-saponins\/\" \/>\n<meta property=\"og:locale\" content=\"fr_FR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Biotransformation of saponins - Longchang Chemical Co., Ltd.\" \/>\n<meta property=\"og:description\" content=\"Saponins are a class of glycosides in which aglycones are triterpene or sterane compounds. They are one of the effective ingredients of many Chinese herbal medicines such as ginseng, licorice and yam (the main saponins structure is shown in Figure 1). Enhance immunity and other functions. There are many reports on the biotransformation of ginsenosides […]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/longchangchemical.com\/fr\/biotransformation-of-saponins\/\" \/>\n<meta property=\"og:site_name\" content=\"Longchang Chemical\" \/>\n<meta property=\"article:published_time\" content=\"2021-03-09T14:01:53+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2024-09-28T11:54:19+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/longchangchemical.com\/wp-content\/uploads\/2021\/03\/image.png\" \/>\n<meta name=\"author\" content=\"Longchang Chemical\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\/\/longchangchemical.com\/biotransformation-of-saponins\/#article\",\"isPartOf\":{\"@id\":\"https:\/\/longchangchemical.com\/biotransformation-of-saponins\/\"},\"author\":{\"name\":\"Longchang Chemical\",\"@id\":\"https:\/\/longchangchemical.com\/#\/schema\/person\/ecb1ad846136c6f1ca534b62978c6a8e\"},\"headline\":\"Biotransformation of saponins\",\"datePublished\":\"2021-03-09T14:01:53+00:00\",\"dateModified\":\"2024-09-28T11:54:19+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\/\/longchangchemical.com\/biotransformation-of-saponins\/\"},\"wordCount\":1601,\"commentCount\":0,\"publisher\":{\"@id\":\"https:\/\/longchangchemical.com\/#organization\"},\"image\":{\"@id\":\"https:\/\/longchangchemical.com\/biotransformation-of-saponins\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/longchangchemical.com\/wp-content\/uploads\/2021\/03\/image.png\",\"articleSection\":[\"Enzyme News\"],\"inLanguage\":\"fr-FR\",\"potentialAction\":[{\"@type\":\"CommentAction\",\"name\":\"Comment\",\"target\":[\"https:\/\/longchangchemical.com\/biotransformation-of-saponins\/#respond\"]}]},{\"@type\":\"WebPage\",\"@id\":\"https:\/\/longchangchemical.com\/biotransformation-of-saponins\/\",\"url\":\"https:\/\/longchangchemical.com\/biotransformation-of-saponins\/\",\"name\":\"Biotransformation of saponins - 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Longchang Chemical Co.","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/longchangchemical.com\/fr\/biotransformation-of-saponins\/","og_locale":"fr_FR","og_type":"article","og_title":"Biotransformation of saponins - Longchang Chemical Co., Ltd.","og_description":"Saponins are a class of glycosides in which aglycones are triterpene or sterane compounds. They are one of the effective ingredients of many Chinese herbal medicines such as ginseng, licorice and yam (the main saponins structure is shown in Figure 1). Enhance immunity and other functions. 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